Click here to switch to the map view.

The map label for this gene is nifS

Identifier: 162146273

GI number: 162146273

Start: 444118

End: 445320

Strand: Direct

Name: nifS

Synonym: GDI_0448

Alternate gene names: 162146273

Gene position: 444118-445320 (Clockwise)

Preceding gene: 162146272

Following gene: 162146274

Centisome position: 11.26

GC content: 66.25

Gene sequence:

>1203_bases
ATGAACGCGGTTTATCTGGACAACAACGCGACGACCCGCGTCGACCCGGCGGCCGTGTCCGCCATGCTGCCGTTCTTTAC
CGAGCAGTTCGGCAACGCATCGTCCATGCATGCGTTCGGCGCCGAGGTCGGGGGTGCCCTGCACACGGCGCGGCGGCAAC
TGCAGGCCCTGCTGGGCGCGGAATTCGATCATGAGATCGTCTATACGGCCGGCGGCACGGAATCCGACAACACCGCCATC
CTCTCGGCGCTGGAGACCCAGGCCGGGCGCAACGAAATCGTGACCAGCGCGGTCGAGCATCCCGCCGTGCTGGCGCTGTG
CGCCTGGCTGGAGAAGACGCGCGGGACCAGGGTGCATTATATCGGCGTCGATGCGCGCGGCCGGCTGGACATCGATGCCT
ATCGCCGTGCCCTGTCGCCGCGCACCGCGATCGCCTCGATCATGTGGGCCAATAACGAGACCGGGACGATCTTCCCGGTC
GAAACGCTGGCGGCCGTGGCGCATGAGGCCGGGGCGCTGTTTCATACCGACGCGGTGCAGGCGGTGGGCAAGATCCCCAT
GAACCTGCGGCATTCGGAAATCGACATGCTGTCGCTCTCGGGGCACAAGCTGCATGCGCCCAAGGGGATTGGCGCGCTGT
ATGTGCGTCGGGGCGTACGCCTGCGTCCGCTGATTCGGGGCGGCCATCAGGAACGCGGCCGGCGCGCGGGCACCGAAAAC
GTACCGGGCATCATCGGCCTGGGCGTGGCGGCCGAACTGGCCCGCCATCACATCGACGAGGAAAATACCCGCGTGCGCGC
CCTGCGGGACCGTCTGGAACAGGGGCTGCTGGAGCGGATCGGCAACGCCTGGGTCACGGGGGATACGGAAAACCGCCTGC
CGAATACGGCGAATGTCGCGTTCGAGTTCATCGAGGGCGAAGCCATCCTGCTGCTGATGAACAAGGCCGGGATCGCGGCG
TCCTCGGGATCGGCCTGCACGTCGGGCTCGCTGGAGCCGTCGCATGTCCTTCGCGCGATGCACGTGCCCTATACCGCGGC
ACACGGCGCGATCCGCTTTTCGTTTTCGCGCGAAAATACGGACGCGGATGTCGATCGCGTTCTGGACGTCATGCCGGGCA
TCATCGCCAAACTGCGTGAAATGTCCCCCTTCTGGGATGGCGCGGCCGCCGCGAAATCCGGCTTTGCACCGACTTATGCC
TAG

Upstream 100 bases:

>100_bases
GCCGTGACGGTGCAGGGCATCCAGGGACGTATCGCCGAACGGCTGGGCACGCCGGTGCGTGTCATTCCGGTGCAGGCCGG
GCAGTGAAGGAGAGCGCGAC

Downstream 100 bases:

>100_bases
GGCGATGCGCCGGGCCTGATGAAGCCCGGCGGTATGGAGGCTGTGCGATGTCGGAAAACGCGCGTCTGCTGATCAGCGAC
ACCACCCTGCGCGATGGAGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNAVYLDNNATTRVDPAAVSAMLPFFTEQFGNASSMHAFGAEVGGALHTARRQLQALLGAEFDHEIVYTAGGTESDNTAI
LSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHYIGVDARGRLDIDAYRRALSPRTAIASIMWANNETGTIFPV
ETLAAVAHEAGALFHTDAVQAVGKIPMNLRHSEIDMLSLSGHKLHAPKGIGALYVRRGVRLRPLIRGGHQERGRRAGTEN
VPGIIGLGVAAELARHHIDEENTRVRALRDRLEQGLLERIGNAWVTGDTENRLPNTANVAFEFIEGEAILLLMNKAGIAA
SSGSACTSGSLEPSHVLRAMHVPYTAAHGAIRFSFSRENTDADVDRVLDVMPGIIAKLREMSPFWDGAAAAKSGFAPTYA

Sequences:

>Translated_400_residues
MNAVYLDNNATTRVDPAAVSAMLPFFTEQFGNASSMHAFGAEVGGALHTARRQLQALLGAEFDHEIVYTAGGTESDNTAI
LSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHYIGVDARGRLDIDAYRRALSPRTAIASIMWANNETGTIFPV
ETLAAVAHEAGALFHTDAVQAVGKIPMNLRHSEIDMLSLSGHKLHAPKGIGALYVRRGVRLRPLIRGGHQERGRRAGTEN
VPGIIGLGVAAELARHHIDEENTRVRALRDRLEQGLLERIGNAWVTGDTENRLPNTANVAFEFIEGEAILLLMNKAGIAA
SSGSACTSGSLEPSHVLRAMHVPYTAAHGAIRFSFSRENTDADVDRVLDVMPGIIAKLREMSPFWDGAAAAKSGFAPTYA
>Mature_400_residues
MNAVYLDNNATTRVDPAAVSAMLPFFTEQFGNASSMHAFGAEVGGALHTARRQLQALLGAEFDHEIVYTAGGTESDNTAI
LSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHYIGVDARGRLDIDAYRRALSPRTAIASIMWANNETGTIFPV
ETLAAVAHEAGALFHTDAVQAVGKIPMNLRHSEIDMLSLSGHKLHAPKGIGALYVRRGVRLRPLIRGGHQERGRRAGTEN
VPGIIGLGVAAELARHHIDEENTRVRALRDRLEQGLLERIGNAWVTGDTENRLPNTANVAFEFIEGEAILLLMNKAGIAA
SSGSACTSGSLEPSHVLRAMHVPYTAAHGAIRFSFSRENTDADVDRVLDVMPGIIAKLREMSPFWDGAAAAKSGFAPTYA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=40, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=32.6923076923077, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI48994898, Length=389, Percent_Identity=42.159383033419, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1789175, Length=390, Percent_Identity=28.7179487179487, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787970, Length=390, Percent_Identity=26.1538461538462, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=388, Percent_Identity=39.6907216494845, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17533177, Length=313, Percent_Identity=32.5878594249201, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=39.5348837209302, Blast_Score=279, Evalue=6e-76,
Organism=Drosophila melanogaster, GI20129463, Length=391, Percent_Identity=41.9437340153453, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFS_GLUDA (P57794)

Other databases:

- EMBL:   AF030414
- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600732.1
- RefSeq:   YP_002275952.1
- ProteinModelPortal:   P57794
- SMR:   P57794
- GeneID:   5791428
- GeneID:   6974968
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1558
- HOGENOM:   HBG635316
- OMA:   AHGTIRF
- ProtClustDB:   CLSK979146
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR03402

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 42978; Mature: 42978

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVYLDNNATTRVDPAAVSAMLPFFTEQFGNASSMHAFGAEVGGALHTARRQLQALLGA
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EFDHEIVYTAGGTESDNTAILSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHY
CCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IGVDARGRLDIDAYRRALSPRTAIASIMWANNETGTIFPVETLAAVAHEAGALFHTDAVQ
EECCCCCCCCHHHHHHHCCCHHHHHHEEECCCCCCCEEEHHHHHHHHHHHCCEEEHHHHH
AVGKIPMNLRHSEIDMLSLSGHKLHAPKGIGALYVRRGVRLRPLIRGGHQERGRRAGTEN
HHHCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHCCCEEEHHHCCCCHHHHCCCCCCC
VPGIIGLGVAAELARHHIDEENTRVRALRDRLEQGLLERIGNAWVTGDTENRLPNTANVA
CCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHE
FEFIEGEAILLLMNKAGIAASSGSACTSGSLEPSHVLRAMHVPYTAAHGAIRFSFSRENT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCC
DADVDRVLDVMPGIIAKLREMSPFWDGAAAAKSGFAPTYA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MNAVYLDNNATTRVDPAAVSAMLPFFTEQFGNASSMHAFGAEVGGALHTARRQLQALLGA
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EFDHEIVYTAGGTESDNTAILSALETQAGRNEIVTSAVEHPAVLALCAWLEKTRGTRVHY
CCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IGVDARGRLDIDAYRRALSPRTAIASIMWANNETGTIFPVETLAAVAHEAGALFHTDAVQ
EECCCCCCCCHHHHHHHCCCHHHHHHEEECCCCCCCEEEHHHHHHHHHHHCCEEEHHHHH
AVGKIPMNLRHSEIDMLSLSGHKLHAPKGIGALYVRRGVRLRPLIRGGHQERGRRAGTEN
HHHCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHCCCEEEHHHCCCCHHHHCCCCCCC
VPGIIGLGVAAELARHHIDEENTRVRALRDRLEQGLLERIGNAWVTGDTENRLPNTANVA
CCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHE
FEFIEGEAILLLMNKAGIAASSGSACTSGSLEPSHVLRAMHVPYTAAHGAIRFSFSRENT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCC
DADVDRVLDVMPGIIAKLREMSPFWDGAAAAKSGFAPTYA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875