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The map label for this gene is emrA [H]

Identifier: 162146068

GI number: 162146068

Start: 247994

End: 249070

Strand: Direct

Name: emrA [H]

Synonym: GDI_0236

Alternate gene names: 162146068

Gene position: 247994-249070 (Clockwise)

Preceding gene: 162146067

Following gene: 162146069

Centisome position: 6.29

GC content: 69.64

Gene sequence:

>1077_bases
ATGATCTACAAGAAACCGCTTCTCTACGCGGGATGCACCGCCTGCGTGCTCGCCCTCGCCGCCTGGGGGGGAACGCGCCT
GGTCAACGGCGACGGCGTCAACCAGTATACCAACGACGCCTATGTCACGGCCGATTTCCCGACCGTGGCGCCCAAGGTGG
CGGGGCGGATCGACCGGGTGATCGCGCAGGACAACCAGGTGGTCCACGCCGGCGAGGAACTGGCCCATATCGAGGACGAC
GACTACCGCGCCGCCCTGGCGGTGGCGACGGGCACCGTGCAGGCCGCCCAGGGCGACGTCGCCAACCTGGAAGCGGAACT
GGCCCGCCAGGACGCCGTGATCGCCCAGGCGCGCGCCGCCGTGCTGGCCGACCAGGCCACCCTGCTGTTCGCGCGGCAGA
ACGCCGTCCGCTACCGCAACCTGTCCTCGGGCGGCGCCGGCACGATCGAACAGCAGCAATCGTCCGACGCGCGTGAAAAG
GAGGCCGAGGCCGCCATCGCCCGCGACCAGGCGGGCGTGGATGCCGCCATCCGGCAGGTCGCGGTGCTGAAGGGACAGAT
CGAACGGGCGCGCGGCGCCCTGCTGCGCGCGCAGGGCGACGAGCGGCAGGCGCAGCTGAACTTGTCCTACTGCACCATTC
CGGCCCCGATGGACGGCGTGGTGGGCGAACGCGGGGTGCGCGTGGGCAATTACGTGCATCCCGGCACGGGCATCCTGGCC
GTGGTGCCGACGCATGATGCCTATGTGCTGGCCAACTTCCAGGAAACGCAGTTGACCAGGGTCCAGACCGGCCAGCGCGC
GACCATCTGGGTCGATACCTTCCCCGGCCAGCCCCTGAAGGCGCACGTGGACTCGCTGGCCCCCGCGACCGGCGTGGCCT
TCGCGCCGATCCAGCCGGACAACGCGACCGGCAACTTCACCAAGGTCGTCCAGCGCATCCCCGTCAAGCTGACCTTCGAT
CCCGGCCAGCCCCTGGCGGCGAAGGTGCGGGTCGGCATGTCGGTCGAAGCGAACATCGACACCTCCTCGAAGCCCGAAGG
CCCGCACGCCAATGATGCCCGTTATGCCTGGCAGTAA

Upstream 100 bases:

>100_bases
CTCGGCTACGCGGTCATGAACCTCATCCTGCCGCGCCGGGTCTATCCGCCGCGCGCGCCGTCGCCGAACACTCCCGCCCG
CTAATCCGGAACGACCGATC

Downstream 100 bases:

>100_bases
CGCCATGACGCGTCGTCTTCTCGCAGGCTTCTCCTGCCTGGCGCTCGTGGGGTGCACGGTCGGGCCGGATTTCCACAAGC
CGCGGGTCACGGCCGCGTCC

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIYKKPLLYAGCTACVLALAAWGGTRLVNGDGVNQYTNDAYVTADFPTVAPKVAGRIDRVIAQDNQVVHAGEELAHIEDD
DYRAALAVATGTVQAAQGDVANLEAELARQDAVIAQARAAVLADQATLLFARQNAVRYRNLSSGGAGTIEQQQSSDAREK
EAEAAIARDQAGVDAAIRQVAVLKGQIERARGALLRAQGDERQAQLNLSYCTIPAPMDGVVGERGVRVGNYVHPGTGILA
VVPTHDAYVLANFQETQLTRVQTGQRATIWVDTFPGQPLKAHVDSLAPATGVAFAPIQPDNATGNFTKVVQRIPVKLTFD
PGQPLAAKVRVGMSVEANIDTSSKPEGPHANDARYAWQ

Sequences:

>Translated_358_residues
MIYKKPLLYAGCTACVLALAAWGGTRLVNGDGVNQYTNDAYVTADFPTVAPKVAGRIDRVIAQDNQVVHAGEELAHIEDD
DYRAALAVATGTVQAAQGDVANLEAELARQDAVIAQARAAVLADQATLLFARQNAVRYRNLSSGGAGTIEQQQSSDAREK
EAEAAIARDQAGVDAAIRQVAVLKGQIERARGALLRAQGDERQAQLNLSYCTIPAPMDGVVGERGVRVGNYVHPGTGILA
VVPTHDAYVLANFQETQLTRVQTGQRATIWVDTFPGQPLKAHVDSLAPATGVAFAPIQPDNATGNFTKVVQRIPVKLTFD
PGQPLAAKVRVGMSVEANIDTSSKPEGPHANDARYAWQ
>Mature_358_residues
MIYKKPLLYAGCTACVLALAAWGGTRLVNGDGVNQYTNDAYVTADFPTVAPKVAGRIDRVIAQDNQVVHAGEELAHIEDD
DYRAALAVATGTVQAAQGDVANLEAELARQDAVIAQARAAVLADQATLLFARQNAVRYRNLSSGGAGTIEQQQSSDAREK
EAEAAIARDQAGVDAAIRQVAVLKGQIERARGALLRAQGDERQAQLNLSYCTIPAPMDGVVGERGVRVGNYVHPGTGILA
VVPTHDAYVLANFQETQLTRVQTGQRATIWVDTFPGQPLKAHVDSLAPATGVAFAPIQPDNATGNFTKVVQRIPVKLTFD
PGQPLAAKVRVGMSVEANIDTSSKPEGPHANDARYAWQ

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=317, Percent_Identity=29.0220820189274, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789041, Length=331, Percent_Identity=30.2114803625378, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1790519, Length=323, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081951, Length=321, Percent_Identity=26.4797507788162, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790024, Length=285, Percent_Identity=27.0175438596491, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789637, Length=295, Percent_Identity=27.7966101694915, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38024; Mature: 38024

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYKKPLLYAGCTACVLALAAWGGTRLVNGDGVNQYTNDAYVTADFPTVAPKVAGRIDRV
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
IAQDNQVVHAGEELAHIEDDDYRAALAVATGTVQAAQGDVANLEAELARQDAVIAQARAA
HHCCCCEEECCCHHHCCCCCCCEEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VLADQATLLFARQNAVRYRNLSSGGAGTIEQQQSSDAREKEAEAAIARDQAGVDAAIRQV
HHHCCCEEEEEECCCHHEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
AVLKGQIERARGALLRAQGDERQAQLNLSYCTIPAPMDGVVGERGVRVGNYVHPGTGILA
HHHHHHHHHHHCCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCEECCEECCCCCEEE
VVPTHDAYVLANFQETQLTRVQTGQRATIWVDTFPGQPLKAHVDSLAPATGVAFAPIQPD
EEECCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCC
NATGNFTKVVQRIPVKLTFDPGQPLAAKVRVGMSVEANIDTSSKPEGPHANDARYAWQ
CCCCHHHHHHHHCCEEEEECCCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIYKKPLLYAGCTACVLALAAWGGTRLVNGDGVNQYTNDAYVTADFPTVAPKVAGRIDRV
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
IAQDNQVVHAGEELAHIEDDDYRAALAVATGTVQAAQGDVANLEAELARQDAVIAQARAA
HHCCCCEEECCCHHHCCCCCCCEEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VLADQATLLFARQNAVRYRNLSSGGAGTIEQQQSSDAREKEAEAAIARDQAGVDAAIRQV
HHHCCCEEEEEECCCHHEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
AVLKGQIERARGALLRAQGDERQAQLNLSYCTIPAPMDGVVGERGVRVGNYVHPGTGILA
HHHHHHHHHHHCCEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCEECCEECCCCCEEE
VVPTHDAYVLANFQETQLTRVQTGQRATIWVDTFPGQPLKAHVDSLAPATGVAFAPIQPD
EEECCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCC
NATGNFTKVVQRIPVKLTFDPGQPLAAKVRVGMSVEANIDTSSKPEGPHANDARYAWQ
CCCCHHHHHHHHCCEEEEECCCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]