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The map label for this gene is oprM [H]

Identifier: 162146069

GI number: 162146069

Start: 249075

End: 250598

Strand: Direct

Name: oprM [H]

Synonym: GDI_0237

Alternate gene names: 162146069

Gene position: 249075-250598 (Clockwise)

Preceding gene: 162146068

Following gene: 162146070

Centisome position: 6.32

GC content: 70.54

Gene sequence:

>1524_bases
ATGACGCGTCGTCTTCTCGCAGGCTTCTCCTGCCTGGCGCTCGTGGGGTGCACGGTCGGGCCGGATTTCCACAAGCCGCG
GGTCACGGCCGCGTCCGGATGGCGGTCCACCCAGCCCCCGGCGGAAAGCCGCGTGACGACGGCCTCGGTCGATCCGGCAT
GGTGGACGCTCTTCAACGACCCGATCCTGACCCGCCTGGAAGCCGAGGTCGCGGCCTCCAACCTCGACCTCCGGGCGGCG
GGCCTGCGGTTCATGCAAAGCGAGGCCGAGCGGCGGATCGCCCGCGCGGCCCAGTTTCCGCACGCCGAAGCCAACGCGTC
CTATGCCCGCGAGCGCGCCAGCACCAACGGCATTCTCGGCCTGCTCGGCACCATGGAGCGCGAAGGGGCCGGGTCCGTGG
CGTCCGGCACCCAGGGGTTCGGACCGACCGCCCTGCCGGGAAGCGTCGGCAATCCCTCGTTCAACCTGCCGCAATATGGC
ATGAGCGCCTCGTGGGAAGTCGACTTCTGGGGCCATGTCCGCCGCCAGGTCGAAGCCGCGACGGCGGCGATGCACGAGAC
CGAGGAGATGCGCCGCGACATCCTCGTCTCCCTGATGGCCGAGACCGCGCAGGATTATATCGACCTGCGCGCCGTCCAGA
CCCAGATCGGCATCCTGAACCATAATATCGACGTGGCCGCCAACAGCGTGAAGCTGACCGGCATGCGCTTCCAGCAGGGC
GCGGCGACCCGGCTGGACGTGGCCGAGGCGACCGGCCAGCTTCACACCTTCACCTCCCGCCTGGCCCCCCTCAAGGCCCA
GGCGACGCACCTGCTCAATGCCCTGAGCTTCCTCGTGGCGCGCCAGCCCGGGGCGCTGGACGCCGAACTGGGGCCGGCGC
GCGACATCCCGTCGGTGCCCGCGACCATTCCCGTCGGGCTGCCGTCCGAACTGGCCGAGCGCCGGCCCGATATCCGCATG
GCCGCCGACCGCCTGCACGCGGCGACGGCCAGCATCGGCGTCGCGATCGCGGACTTCTTCCCCCGGATCACGCTGTCCGG
CAGCCTCGACGTCCAGGCGCTGCAGTTCAGCGGCCTGGGGTCCTGGGCGTCGCGCCAGTACGGGTTCGGCCCCACCGCGA
CGCTGCCGATCTTCGAGGGCGGCCGCCTGACGGGGCAGTTGCGCCTGCGCCGCGCGCAGCAGCAGGAAGCCGCCACGATG
TTCCAGCGGACGGTCCTGAAGGCGTGGCAGGAAATCGACGACGCGATGGCCGATTTCACGGCCGCGCAGCAGCGGCGCGA
CGAACTGGCCGAAGCGGTGCGGGAGAACGAAATTGCGGTGGACACGGCCAGGGCGCAATATGTCCAGGGATCGTCGGACT
TCCTGAACGTCCTGACCCTGCAGAACGCGCTCCTGTCGTCCCAGAGCGCCCTGGCCGACGCGACGGCCACCGTGGCCGGG
TCGGTCACCCACCTCTACCGCGCGGTCGGCGGGGGATGGGAAGGGCTGTATCCGGAAAAGCCGAATCACCGGCCCCCTGC
GTAG

Upstream 100 bases:

>100_bases
AAGGTGCGGGTCGGCATGTCGGTCGAAGCGAACATCGACACCTCCTCGAAGCCCGAAGGCCCGCACGCCAATGATGCCCG
TTATGCCTGGCAGTAACGCC

Downstream 100 bases:

>100_bases
AGGACGAGGACATGATCACGATCCGACGGTCGGACACGCTGGGCACGGCACGGTCCGACGGCCTGGCCATCCGGTGCCAT
TTCGCGTTCGCCGACTGGCA

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTRRLLAGFSCLALVGCTVGPDFHKPRVTAASGWRSTQPPAESRVTTASVDPAWWTLFNDPILTRLEAEVAASNLDLRAA
GLRFMQSEAERRIARAAQFPHAEANASYARERASTNGILGLLGTMEREGAGSVASGTQGFGPTALPGSVGNPSFNLPQYG
MSASWEVDFWGHVRRQVEAATAAMHETEEMRRDILVSLMAETAQDYIDLRAVQTQIGILNHNIDVAANSVKLTGMRFQQG
AATRLDVAEATGQLHTFTSRLAPLKAQATHLLNALSFLVARQPGALDAELGPARDIPSVPATIPVGLPSELAERRPDIRM
AADRLHAATASIGVAIADFFPRITLSGSLDVQALQFSGLGSWASRQYGFGPTATLPIFEGGRLTGQLRLRRAQQQEAATM
FQRTVLKAWQEIDDAMADFTAAQQRRDELAEAVRENEIAVDTARAQYVQGSSDFLNVLTLQNALLSSQSALADATATVAG
SVTHLYRAVGGGWEGLYPEKPNHRPPA

Sequences:

>Translated_507_residues
MTRRLLAGFSCLALVGCTVGPDFHKPRVTAASGWRSTQPPAESRVTTASVDPAWWTLFNDPILTRLEAEVAASNLDLRAA
GLRFMQSEAERRIARAAQFPHAEANASYARERASTNGILGLLGTMEREGAGSVASGTQGFGPTALPGSVGNPSFNLPQYG
MSASWEVDFWGHVRRQVEAATAAMHETEEMRRDILVSLMAETAQDYIDLRAVQTQIGILNHNIDVAANSVKLTGMRFQQG
AATRLDVAEATGQLHTFTSRLAPLKAQATHLLNALSFLVARQPGALDAELGPARDIPSVPATIPVGLPSELAERRPDIRM
AADRLHAATASIGVAIADFFPRITLSGSLDVQALQFSGLGSWASRQYGFGPTATLPIFEGGRLTGQLRLRRAQQQEAATM
FQRTVLKAWQEIDDAMADFTAAQQRRDELAEAVRENEIAVDTARAQYVQGSSDFLNVLTLQNALLSSQSALADATATVAG
SVTHLYRAVGGGWEGLYPEKPNHRPPA
>Mature_506_residues
TRRLLAGFSCLALVGCTVGPDFHKPRVTAASGWRSTQPPAESRVTTASVDPAWWTLFNDPILTRLEAEVAASNLDLRAAG
LRFMQSEAERRIARAAQFPHAEANASYARERASTNGILGLLGTMEREGAGSVASGTQGFGPTALPGSVGNPSFNLPQYGM
SASWEVDFWGHVRRQVEAATAAMHETEEMRRDILVSLMAETAQDYIDLRAVQTQIGILNHNIDVAANSVKLTGMRFQQGA
ATRLDVAEATGQLHTFTSRLAPLKAQATHLLNALSFLVARQPGALDAELGPARDIPSVPATIPVGLPSELAERRPDIRMA
ADRLHAATASIGVAIADFFPRITLSGSLDVQALQFSGLGSWASRQYGFGPTATLPIFEGGRLTGQLRLRRAQQQEAATMF
QRTVLKAWQEIDDAMADFTAAQQRRDELAEAVRENEIAVDTARAQYVQGSSDFLNVLTLQNALLSSQSALADATATVAGS
VTHLYRAVGGGWEGLYPEKPNHRPPA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=495, Percent_Identity=26.8686868686869, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI2367348, Length=473, Percent_Identity=23.8900634249471, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54536; Mature: 54405

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRLLAGFSCLALVGCTVGPDFHKPRVTAASGWRSTQPPAESRVTTASVDPAWWTLFND
CCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCEEECCCCHHEEEECC
PILTRLEAEVAASNLDLRAAGLRFMQSEAERRIARAAQFPHAEANASYARERASTNGILG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHH
LLGTMEREGAGSVASGTQGFGPTALPGSVGNPSFNLPQYGMSASWEVDFWGHVRRQVEAA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
TAAMHETEEMRRDILVSLMAETAQDYIDLRAVQTQIGILNHNIDVAANSVKLTGMRFQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEECEEECCC
AATRLDVAEATGQLHTFTSRLAPLKAQATHLLNALSFLVARQPGALDAELGPARDIPSVP
CCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ATIPVGLPSELAERRPDIRMAADRLHAATASIGVAIADFFPRITLSGSLDVQALQFSGLG
CEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCC
SWASRQYGFGPTATLPIFEGGRLTGQLRLRRAQQQEAATMFQRTVLKAWQEIDDAMADFT
CHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQQRRDELAEAVRENEIAVDTARAQYVQGSSDFLNVLTLQNALLSSQSALADATATVAG
HHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTHLYRAVGGGWEGLYPEKPNHRPPA
HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRLLAGFSCLALVGCTVGPDFHKPRVTAASGWRSTQPPAESRVTTASVDPAWWTLFND
CCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCEEECCCCHHEEEECC
PILTRLEAEVAASNLDLRAAGLRFMQSEAERRIARAAQFPHAEANASYARERASTNGILG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHH
LLGTMEREGAGSVASGTQGFGPTALPGSVGNPSFNLPQYGMSASWEVDFWGHVRRQVEAA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
TAAMHETEEMRRDILVSLMAETAQDYIDLRAVQTQIGILNHNIDVAANSVKLTGMRFQQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEECEEECCC
AATRLDVAEATGQLHTFTSRLAPLKAQATHLLNALSFLVARQPGALDAELGPARDIPSVP
CCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ATIPVGLPSELAERRPDIRMAADRLHAATASIGVAIADFFPRITLSGSLDVQALQFSGLG
CEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCC
SWASRQYGFGPTATLPIFEGGRLTGQLRLRRAQQQEAATMFQRTVLKAWQEIDDAMADFT
CHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQQRRDELAEAVRENEIAVDTARAQYVQGSSDFLNVLTLQNALLSSQSALADATATVAG
HHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTHLYRAVGGGWEGLYPEKPNHRPPA
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]