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The map label for this gene is ldhA [H]

Identifier: 161870533

GI number: 161870533

Start: 1628202

End: 1629203

Strand: Reverse

Name: ldhA [H]

Synonym: NMCC_1598

Alternate gene names: 161870533

Gene position: 1629203-1628202 (Counterclockwise)

Preceding gene: 161870534

Following gene: 161870531

Centisome position: 75.66

GC content: 52.79

Gene sequence:

>1002_bases
TTGATGAAGATTGCGATTTACGGTACGAAAAGCTATGACCGCGAACATTTTACCCGCGCCAACCGGCATTTCGGCTTCGA
GCTGGAGTTTTTTGATTTTATGCTGGATGCGAAAACGGCGAAAATGGCGGAAGGCGCGGAAGCGGTTTGTATTTTTGTAA
ACGACGACGGCAGCCGGCCCGTGTTGGAAAAATTGGCGCAAATTGGCGTGAAAACTGTGGCGTTGCGTTGTGCCGGCTTC
AATAATGTGGATTTGAAAGCGGCTGAAGAGTTGGGCTTGAAAGTCGTGCGCGTGCCTGCCTATTCGCCCGAATCGGTTGC
GGAACATACGGTCGGTCTGATGCTGACGTTGAACCGCCGTATCCACAAAGCCTATCAGCGTACCCGCGATGCGAATTTTT
CGCTGGAAGGTCTGACCGGTTTCAATATGTACGGCAAAACGGCGGGCGTTATCGGCACGGGGAAAATCGGTATCGCAACG
ATGCGGATTTTAAAGGGTTTCGGTATGAACCTGCTGGCTTACGATCCGTTTTGCAACCCCGAAGTGGAAAAACTCGGCGG
AAAATATGTGGATTTGGACGAGCTGTATGCCCAGTCGGACATCATCACGCTGCATTGCCCCTCCACGCCGGAAAACCACT
ATATGCTGAACGAAGCGGCGTTCGACAAAATGAAAGACGGCGTGATGATTATCAACACCAGCCGGGGCGGGCTGATTGAC
AGTGCCGCCGCGATTGAGGCGTTGAAACGCCGGAAAATCGGCGCATTGGGCATGGATGTGTACGAGAACGAGCGCGAGCT
GTTTTTTGAAGACAAATCCAACGATGTGATTACCGACGACGTGTTCCGCCGCCTGTCGTCTTGTCATAATGTGCTGTTTA
CCGGCCATCAGGCATTTTTGACGGAAGAGGCGTTGGGTAATATTTCGGAAGTTACGCTGTCCAATATCCGCGAGGTCGGA
CAGACCGGCGATTGCGGCAATGCGGTTCGTGCCGACGGCTGA

Upstream 100 bases:

>100_bases
ATGCCGTGCATGCAATCAATTTTAATCCACCATATTCTACTGATTTGTTTGAATTCAGTGTATTATGCCGTTTCTCAACC
ATAGACGACAACTGGACATA

Downstream 100 bases:

>100_bases
CGTGTTTTAAAAGTTTAAAGGAAATGCCGTCTGAACCAAGTTTCAGACGGCATTTATGTTCAGGGTTAAGGCTTATTTCA
CTTCCAACTTGGCAACGCCG

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MMKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEGAEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGF
NNVDLKAAEELGLKVVRVPAYSPESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIAT
MRILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEAAFDKMKDGVMIINTSRGGLID
SAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVITDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVG
QTGDCGNAVRADG

Sequences:

>Translated_333_residues
MMKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEGAEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGF
NNVDLKAAEELGLKVVRVPAYSPESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIAT
MRILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEAAFDKMKDGVMIINTSRGGLID
SAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVITDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVG
QTGDCGNAVRADG
>Mature_333_residues
MMKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEGAEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGF
NNVDLKAAEELGLKVVRVPAYSPESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIAT
MRILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEAAFDKMKDGVMIINTSRGGLID
SAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVITDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVG
QTGDCGNAVRADG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=34.136546184739, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=34.136546184739, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=32.5301204819277, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=32.5301204819277, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI23308577, Length=233, Percent_Identity=32.1888412017167, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI145580575, Length=249, Percent_Identity=32.5301204819277, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI6912396, Length=226, Percent_Identity=30.0884955752212, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=72.6993865030675, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI87082289, Length=216, Percent_Identity=30.0925925925926, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789279, Length=251, Percent_Identity=30.6772908366534, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788660, Length=212, Percent_Identity=26.8867924528302, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=266, Percent_Identity=30.8270676691729, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=202, Percent_Identity=30.1980198019802, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=234, Percent_Identity=34.6153846153846, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=234, Percent_Identity=33.7606837606838, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=270, Percent_Identity=29.6296296296296, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.921568627451, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=33.7209302325581, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=33.7209302325581, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=33.7209302325581, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=33.7209302325581, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=33.7209302325581, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921140, Length=236, Percent_Identity=30.5084745762712, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574286, Length=238, Percent_Identity=28.9915966386555, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571528, Length=281, Percent_Identity=28.1138790035587, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585514, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574282, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45552429, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28574284, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45551003, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=25.1256281407035, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36792; Mature: 36792

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEGAEAVCIFVNDDGSRP
CEEEEEECCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
VLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSPESVAEHTVGLMLTLNRR
HHHHHHHCCHHEEEEEECCCCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHEEEEEHHHH
IHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATMRILKGFGMNLLAYDPFCNP
HHHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCCC
EVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEAAFDKMKDGVMIINTSRGGLID
HHHHHCCCEECHHHHHCCCCEEEEECCCCCCCCEEECHHHHHHHCCCEEEEECCCCCCCH
SAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVITDDVFRRLSSCHNVLFTGHQAFL
HHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHEEECCCHHHH
TEEALGNISEVTLSNIREVGQTGDCGNAVRADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEGAEAVCIFVNDDGSRP
CEEEEEECCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
VLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSPESVAEHTVGLMLTLNRR
HHHHHHHCCHHEEEEEECCCCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHEEEEEHHHH
IHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATMRILKGFGMNLLAYDPFCNP
HHHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEECCCCCC
EVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEAAFDKMKDGVMIINTSRGGLID
HHHHHCCCEECHHHHHCCCCEEEEECCCCCCCCEEECHHHHHHHCCCEEEEECCCCCCCH
SAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVITDDVFRRLSSCHNVLFTGHQAFL
HHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHEEECCCHHHH
TEEALGNISEVTLSNIREVGQTGDCGNAVRADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]