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The map label for this gene is prfA [H]

Identifier: 161870534

GI number: 161870534

Start: 1629325

End: 1630401

Strand: Reverse

Name: prfA [H]

Synonym: NMCC_1599

Alternate gene names: 161870534

Gene position: 1630401-1629325 (Counterclockwise)

Preceding gene: 161870535

Following gene: 161870533

Centisome position: 75.71

GC content: 57.85

Gene sequence:

>1077_bases
ATGAAGCCGTCTATCTTAGAAAAACTGCAACAACTCGGCGACCGGTTGGAAGAAGTTACCCACCTTTTAGGGCAACCCGA
AGCCACGGCAGATATGGACAACTACCGCAAGCTCACGCGCGAACACGCCGAACTGACCCCCGTCGTCGAAGTGTTCCAAA
ACTATCGGCTTGCCCAAAGCGACTTGGCGGATGCCGAAGAAATGTTGTCCGACCCCGAAATGAAAGACTTTGCCGCCGAA
GAAATCGAAGCGGCGAAAGCCAAAATCGGCGCACTCGATACCGAATTGCAAAAACTGCTGCTGCCCAAAGATGCCGACGA
CGACAAAAACATCTTTATCGAAGTACGCGCCGGAACGGGCGGCGACGAAGCCGCGCTGTTTGCTGGCGATTTGCTGCGTA
TGTACAGCCGCTACGCCGAACGCAACCGCTGGCAGGTTGAAATCGTGTCCGCCAACGAAAGCGAGTTGGGCGGCTATAAA
GAAGTTATCGCCCGTATTATCGGCTTAGGCGCGTACAGCCGTTTGAAATTTGAATCGGGCGGACACCGCGTGCAGCGCGT
CCCCGCCACCGAAAGCCAAGGCCGCATCCACACCTCCGCCTGCACCGTTGCCGTCATGCCCGAAGCGGACGAACTGGAAG
ACATCGAGTTGAACCCCGTCGACCTTCGCATCGACACCTTCCGCGCATCCGGCGCAGGCGGTCAGCACATCAACAAAACC
GATTCCGCCGTCCGCATTACCCACCTGCCCACCGGCATGGTGGTCGAATGCCAAGACGGCCGCAGCCAACACGCCAACAA
AGCGCAGGCAATGAAAGTTCTCGCCGCCCGCCTGAACGACGCGCAAAAACGCGAAGCCCAAGCCAAAGAAGCCGCCGAAC
GCAAATCCCTCATCGGCAGCGGCGACCGCAGCGAACGCATCCGCACCTACAACTATCCCCAAGGCAGGGTAACAGACCAC
CGCATCAACCTCACCCTGCACAAGCTGGATTTTGTGATGGACGGCGATTTGGCAGAAATCACCGATGCCCTGATTGCCGA
ACATCAGGCGGAGCTTTTGGCGGCAATGGGCGATTGA

Upstream 100 bases:

>100_bases
TCCGTCCCTAGATTATCTTTTTGAATATTCAGAAAATATATTATAATGACCGCGTTTTGATGCCGTCTGAAATTCAGGCG
GCGTTTGTTATGGAAGAATT

Downstream 100 bases:

>100_bases
GTTTTGTGTTTTGAAATGGAAATGCCGTGCATGCAATCAATTTTAATCCACCATATTCTACTGATTTGTTTGAATTCAGT
GTATTATGCCGTTTCTCAAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAELTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAE
EIEAAKAKIGALDTELQKLLLPKDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELNPVDLRIDTFRASGAGGQHINKT
DSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAARLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDH
RINLTLHKLDFVMDGDLAEITDALIAEHQAELLAAMGD

Sequences:

>Translated_358_residues
MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAELTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAE
EIEAAKAKIGALDTELQKLLLPKDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELNPVDLRIDTFRASGAGGQHINKT
DSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAARLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDH
RINLTLHKLDFVMDGDLAEITDALIAEHQAELLAAMGD
>Mature_358_residues
MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAELTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAE
EIEAAKAKIGALDTELQKLLLPKDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELNPVDLRIDTFRASGAGGQHINKT
DSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAARLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDH
RINLTLHKLDFVMDGDLAEITDALIAEHQAELLAAMGD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=47.8688524590164, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI34577120, Length=301, Percent_Identity=43.1893687707641, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=47.6923076923077, Blast_Score=185, Evalue=7e-47,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=63.4078212290503, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=38.5074626865672, Blast_Score=216, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=276, Percent_Identity=38.768115942029, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=42.5396825396825, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19921226, Length=337, Percent_Identity=40.0593471810089, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39660; Mature: 39660

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAELTPVVEVFQNYRLAQS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLPKDADDDKNIFIEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYKEVIARIIGLGAYSRLKFESG
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCCHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELNPVDLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAARLNDAQKREAQAKEAAERKSLIGS
CCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMDGDLAEITDALIAEHQAELLAAMGD
CCCCCCCEECCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAELTPVVEVFQNYRLAQS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLPKDADDDKNIFIEVRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYKEVIARIIGLGAYSRLKFESG
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCCHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELNPVDLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAARLNDAQKREAQAKEAAERKSLIGS
CCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMDGDLAEITDALIAEHQAELLAAMGD
CCCCCCCEECCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA