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The map label for this gene is tgt [H]

Identifier: 161869661

GI number: 161869661

Start: 691421

End: 692536

Strand: Reverse

Name: tgt [H]

Synonym: NMCC_0678

Alternate gene names: 161869661

Gene position: 692536-691421 (Counterclockwise)

Preceding gene: 161869685

Following gene: 161869660

Centisome position: 32.16

GC content: 54.12

Gene sequence:

>1116_bases
ATGCTTAAATTTACTTTACACAAAAAAGACGGATACGCCCGACGCGGCACGCTGGAGTTGAACCACGGCAAAATTGAAAC
GCCGGTATTTATGCCTGTCGGTACTTACGGCTCGGTTAAGGCGATGAACCCGCAAAACCTGCACGACATCAAGGCGCAAA
TTATTTTGGGCAACACTTATCATTTGTGGCTGCGTCCGGGTTTGGAAGTTATCGGGCAATTCGGCGGCCTGCACGGTTTT
ATCGGCTGGGACAAACCGATTTTGACCGACTCGGGCGGTTTTCAGGTTTTTTCTTTGTCGGATATGCGCAAGCTGACGGA
AGAAGGCTGTACGTTCAAAAGCCCGATTAACGGCGACAAGCTGTTTTTATCGCCTGAAATTTCTATGAAAATCCAAACGG
TTTTGAACTCGGACATCGCGATGCAGTTGGACGAATGCACGCCGGGCGAGGCGACGCGCGAACAGGCGCGAAAATCGCTG
CAAATGAGCCTGCGCTGGGCGGAACGGAGCAAAAAAGCCTTTGAAGATTTGAAAAACCCGAACGCACTGTTCGGCATCGT
GCAAGGCGCGATGTATGAGGATTTGCGCGAAGAATCGCTGCGCGGTTTGGAACAGTTTGATTTTCCCGGCCTTGCCGTCG
GCGGTTTGTCCGTCGGCGAACCCAAGCCCGAAATGTACCGTATGCTGCGCGCCGTCGGTCCGATTCTGCCGGAACACAAG
CCGCATTACCTGATGGGCGTAGGCACGCCGGAAGACCTCGTGTACGGCGTGGCACACGGCATCGATATGTTCGACTGCGT
GATGCCCACCCGCAATGCACGCAACGGCTGGCTGTTCACCCGTTTCGGCGATTTGAAAATCAAAAACGCCAAACACAAGC
TCGACAAGCGTCCGATAGACGAAAGCTGCACCTGCTACGCCTGCCAAAACTTCAGCCGCGCCTACCTGCACCATCTGCAC
CGCGCCGGCGAAATCTTGGGCGCACAGCTCAACACCATCCACAACCTGCATTTCTACCAAGTCATTATGGCGGAAATGCG
CGATGCCATAGAACAAGGCAAATTTGCCGACTGGCAGGCACAATTCCACGAAAACCGCGCACGCGGCACAGACTGA

Upstream 100 bases:

>100_bases
AAGATTTGGGGATGTCGTCTGAAAAACCGTGGGAATTCATTTATAATGCCGTCTTTTAAAACATATTTCGCCCCCTGTCG
CCCAACTGACATTCAATCTT

Downstream 100 bases:

>100_bases
TTCCCGGCAATGCCGTCTGAAGCCGCTTCAGACGGCATTTTGCCGCTTTACCCTACCCGCACTATTTTTCAGAAACCGAT
CATGCTTCCATTCCTTCCAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHK
PHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLH
RAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD

Sequences:

>Translated_371_residues
MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHK
PHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLH
RAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD
>Mature_371_residues
MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGF
IGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHK
PHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLH
RAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=42.5414364640884, Blast_Score=299, Evalue=2e-81,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=60.2179836512262, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=40.8839779005525, Blast_Score=283, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920510, Length=350, Percent_Identity=43.7142857142857, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI21355311, Length=250, Percent_Identity=26, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41938; Mature: 41938

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTY
CCEEEEECCCCCCCCCEEEECCCEECCCEEEEECCCCCEEECCCCCCCCCEEEEEECCEE
HLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDK
EEEECCCHHHHHHHCCCCEECCCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCE
LFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNP
EEECCCCCEEHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
PHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPID
CCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCEEECCHHHHHCCCCCC
ESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QFHENRARGTD
HHHHCCCCCCC
>Mature Secondary Structure
MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNPQNLHDIKAQIILGNTY
CCEEEEECCCCCCCCCEEEECCCEECCCEEEEECCCCCEEECCCCCCCCCEEEEEECCEE
HLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDK
EEEECCCHHHHHHHCCCCEECCCCCCEEECCCCEEEEEHHHHHHHHHCCCEECCCCCCCE
LFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNP
EEECCCCCEEHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSVGEPKPEMYRMLRAVGPILPEHK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
PHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPID
CCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCEEECCHHHHHCCCCCC
ESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QFHENRARGTD
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA