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The map label for this gene is mnmC [H]

Identifier: 161869685

GI number: 161869685

Start: 714122

End: 715741

Strand: Reverse

Name: mnmC [H]

Synonym: NMCC_0705

Alternate gene names: 161869685

Gene position: 715741-714122 (Counterclockwise)

Preceding gene: 161869689

Following gene: 161869661

Centisome position: 33.24

GC content: 58.7

Gene sequence:

>1620_bases
ATGGACAATCTCGTATGGGACGGCGTTCCCGACATCCGCACACTCGACCAAACCATCCGCAAACACGCACACCCGCTCAA
CCTGATTGTCTGCCTCCCCGATAATCAGATTCCCGATTTTCAAACCGCACAAGATGCTTCGGACGCGGAATGCCGTCTGA
AGCACCGTTTGGATCAAGCAACGCAGTGCCTCCAGTTCGACAGCATCAACCTCATCGAACACATCCTGCCCGATGTCCGC
TTCTGGCTGGTTCCCCCTTCACGCACCCGCCGCCTGCACGAACACTTCCACCACATTTCCTGGCAGACCGAAGCCATCCC
GCAAACCGAAAGTAAGCCCGACAAACCCTGGTTTGCACTTCCACAAACATCCGAACGGCAAAAACCGGAACACATCCTCG
TTATCGGCGCGGGCATATCCGGCGCGGCAACCGCCCACGCCTTAGCATCATACGGCATTTCCGTTACCGTATTGGAAGCC
CGAAAAGCCGCCCAAGCCGCCAGCGGCAACCGCCAAGGGCTGCTCTACGCCAAAATCTCGCCACACAATACCGAACAAAC
CGAACTTTTGCTCACCGGCTACGGCTACACCAAACGCCTGCTCGGACACATCCTGCCCGAATCCGAAACCTGGGGCGGCA
ACGGCATCATCCACCTCAATTACAGCCGCACCGAACAACAACGCAATCACGAATTGGGTTTGCAAAAACACCATAACCAC
CTCTACCGCAGCATCACGCAGGCAGAAGCCGAAAAAATCGCCGGTATCCCACTGTCCGTCCCATACGACCACCCTTCATG
CGGACTCTACTGGCAACACGGCGTATGGCTCAATCCACCCGCATTCGTCCGCGCCCTCCTCAACCATCCGCTCATTGGAC
TACACGAAGACACACCGTTAACCGACATTTCCCACGACGGGGAAAAGTGGATTGCAAGCACGCCAAACGGCATATTTTCC
GCCACACACATCATCTACTGCACCGGCGCGCACAGCTCCTGCCTGCCCGAAACCAACCTCGCCGCCCTGCCTCTCAGGCA
AATACGCGGACAAACCGGCCTCACACCATCCACCCCGTTTTCCGAACAACTGCGTTGCGCCGTTTCAGGCGAAAGCTACA
TCAGCCCGTCGTGGCACGGACTGCACTGCTACGGCGCGAGTTTTATTCCCAACAGCAGCCATACCGGATGGAACGAAGCC
GAAGAAGCCTCAAACCGCCAAGCATTGGCACAGCTTAGCCCCGCCCTCGCCGAATCATTGTTTGCCGCCAACCCAATCCC
CCAAAAACACCAAGGGCACGCCGCCATGCGCTGCGACAGCCCCGACCACCTTCCCCTAGTCGGCGCACTCGGCGACATTG
CCGCTATGCAACAAACTTACGCCAAACTCGCGCTGGACAAAAACTATCGCATCGACGCCCCCTGCCCGTACCTGCCCAAT
GCCTACGCCAACACCGCCCACGGCACACGCGGGCTTGCCACCGCCCCCATCTGCGCCGCCGCCGTTGCAGCCGAAATCCT
AGGCTTGCCCCATCCCCTCTCAAAACGCCTGCGCCACGCCCTACACCCCAACCGCACCATCATCCGCGCCATCGTCAGAA
GGAAGGATCTAACCCCTTAA

Upstream 100 bases:

>100_bases
TACAATATCCGGCAGGCGCAGGCGTGCCTTCAAACGGCGCGTATTTTAACAGCTTTATCCCGATACCGATATTCAAACCC
ACACTTTAAACGGCATTGAT

Downstream 100 bases:

>100_bases
GCCTTTGCCGCATGCCGTCCCGACCACAGAAAATCATATTCAAATAAACATACCAATCCTGACGGTAAAATATCGAAAAC
ACCCATAACGTTTTCTTCCC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQDASDAECRLKHRLDQATQCLQFDSINLIEHILPDVR
FWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH
LYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFS
ATHIIYCTGAHSSCLPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNSSHTGWNEA
EEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN
AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRTIIRAIVRRKDLTP

Sequences:

>Translated_539_residues
MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQDASDAECRLKHRLDQATQCLQFDSINLIEHILPDVR
FWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH
LYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFS
ATHIIYCTGAHSSCLPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNSSHTGWNEA
EEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN
AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRTIIRAIVRRKDLTP
>Mature_539_residues
MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQDASDAECRLKHRLDQATQCLQFDSINLIEHILPDVR
FWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH
LYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFS
ATHIIYCTGAHSSCLPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNSSHTGWNEA
EEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN
AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRTIIRAIVRRKDLTP

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=478, Percent_Identity=26.5690376569038, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 59464; Mature: 59464

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQDASDAECRLKHRLDQA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFAL
HHHHHCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEC
PQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEARKAAQAASGNRQGLLYAKIS
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCEEEEEEEC
PHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH
CCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHH
LYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRALLNHPLIGLHEDTPL
HHHHHHHHHHHHHCCCCEECCCCCCCCCEEEECCEEECCHHHHHHHHHCCEEEECCCCCC
TDISHDGEKWIASTPNGIFSATHIIYCTGAHSSCLPETNLAALPLRQIRGQTGLTPSTPF
CCCCCCCCHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHCCHHHHCCCCCCCCCCCH
SEQLRCAVSGESYISPSWHGLHCYGASFIPNSSHTGWNEAEEASNRQALAQLSPALAESL
HHHHEEEECCCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAANPIPQKHQGHAAMRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN
HHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRTIIRAIVRRKDLTP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQDASDAECRLKHRLDQA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFAL
HHHHHCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEC
PQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEARKAAQAASGNRQGLLYAKIS
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCCEEEEEEEC
PHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH
CCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHH
LYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRALLNHPLIGLHEDTPL
HHHHHHHHHHHHHCCCCEECCCCCCCCCEEEECCEEECCHHHHHHHHHCCEEEECCCCCC
TDISHDGEKWIASTPNGIFSATHIIYCTGAHSSCLPETNLAALPLRQIRGQTGLTPSTPF
CCCCCCCCHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHCCHHHHCCCCCCCCCCCH
SEQLRCAVSGESYISPSWHGLHCYGASFIPNSSHTGWNEAEEASNRQALAQLSPALAESL
HHHHEEEECCCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAANPIPQKHQGHAAMRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN
HHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRTIIRAIVRRKDLTP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA