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The map label for this gene is prfA [H]

Identifier: 161526057

GI number: 161526057

Start: 3153242

End: 3154324

Strand: Direct

Name: prfA [H]

Synonym: Bmul_2888

Alternate gene names: 161526057

Gene position: 3153242-3154324 (Clockwise)

Preceding gene: 161526056

Following gene: 161526058

Centisome position: 91.44

GC content: 66.3

Gene sequence:

>1083_bases
ATGAAGACGAGCATGCAACGCAAGCTCGACCAGCTGTCCACCCGGCTGGCCGAACTGAACGATCTGCTGAGCCGCGAAAA
CGTCACGGCCGATCTCGACCAGTACCGCAAGCTCACGCGCGAACACGCGGAACTCGGCCCGGTCGTCGAGCAGTACGCGC
TGTGGCGCCAGTCGCGCAGCGACGAGACGGCCGCGCAGGAATTGCTCGCCGATCCGTCGATGCGCGATTTCGCCGAGGAA
GAAATCCGCAGCGCGCGCGAGCGGATGGCGCGGCTCGAGGTCGAGCTGCAGAAGATGCTGCTGCCGAAGGATCCGAACGA
CGATCGCAACATCTTCGTCGAAATCCGCGCGGGCACCGGCGGCGACGAATCGGCGCTGTTCGCCGGCGATCTGCTGCGCA
TGTACCTGCGCTTTGCGGAACGGCAGCGCTGGCAGGTCGAGATGATGTCGGAAAGCCCGTCCGATCTCGGCGGCTACAAG
GAAGTGATCGTGCGGATCGCCGGCCAGGGCGCGTATTCGCGGCTGAAGTTCGAGTCGGGCGGCCATCGCGTGCAGCGCGT
GCCGGCCACCGAGACGCAGGGGCGCATCCATACGTCCGCGTGCACGGTCGCCGTGATGCCGGAGGCGGACGAAATCGGCG
AAGTCGAGATCAACCTGGCCGACTTGCGGATCGACACGTTCCGCGCGTCGGGCGCGGGCGGCCAGCACATCAACAAGACC
GATTCCGCGGTGCGTGTCACGCACCTGCCGACCGGGATCGTCGTCGAGTGCCAGGACGACCGCTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAGGTGCTCGCCGCGCGCATCAAGGACAAGCAGTATCACGAGCAGCACGCGAAGGAAGCCGCGACGC
GCAAGAGCCTGATCGGCTCGGGCGACCGTTCCGAGCGGATCCGCACGTACAACTTCCCGCAGGGCCGGATGACCGATCAC
CGGATCAACCTGACGCTCTACCGGCTCGAGGCGATCATGGACGGCGATCTCGACGAACTGATCGGCGCGCTCGTCAGCGA
GCATCAGGCCGAGCTGCTCGCGTCGCTCGGCGACGCCGACTGA

Upstream 100 bases:

>100_bases
GATCAGTCCGGCCACTAGCGCCGGCCGATCGCACTGCCGCCGGCCGTCGGCCGGCCTATCCTTTCCGAATACTCCTTGCC
CGGGAGCGCCGCTCCACACC

Downstream 100 bases:

>100_bases
GGCCGCGACGATGTCCGCACCGACTGCCGACGACCTGCTGCGCGCGACGCCGCTCGATGCGGTCGACGCGCGCGTGCTGC
TCGCGCACGCGCTCGGCTGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAEE
EIRSARERMARLEVELQKMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESPSDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINLADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVSEHQAELLASLGDAD

Sequences:

>Translated_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAEE
EIRSARERMARLEVELQKMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESPSDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINLADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVSEHQAELLASLGDAD
>Mature_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLADPSMRDFAEE
EIRSARERMARLEVELQKMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESPSDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINLADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIGALVSEHQAELLASLGDAD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=340, Percent_Identity=45.2941176470588, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=38.0053908355795, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=245, Percent_Identity=42.0408163265306, Blast_Score=181, Evalue=8e-46,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=60.8938547486033, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.3233532934132, Blast_Score=218, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=295, Percent_Identity=37.2881355932203, Blast_Score=191, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=40.2266288951841, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI19921226, Length=266, Percent_Identity=44.7368421052632, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40641; Mature: 40641

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLADPSMRDFAEEEIRSARERMARLEVELQKMLLPKDPNDDRNIFVEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESPSDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINLADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCC
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIGALVSEHQAELLASLGDAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLADPSMRDFAEEEIRSARERMARLEVELQKMLLPKDPNDDRNIFVEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESPSDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINLADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCC
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIGALVSEHQAELLASLGDAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA