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The map label for this gene is hemK [H]

Identifier: 161526058

GI number: 161526058

Start: 3154335

End: 3155177

Strand: Direct

Name: hemK [H]

Synonym: Bmul_2889

Alternate gene names: 161526058

Gene position: 3154335-3155177 (Clockwise)

Preceding gene: 161526057

Following gene: 161526059

Centisome position: 91.47

GC content: 72.95

Gene sequence:

>843_bases
ATGTCCGCACCGACTGCCGACGACCTGCTGCGCGCGACGCCGCTCGATGCGGTCGACGCGCGCGTGCTGCTCGCGCACGC
GCTCGGCTGGACGCGCACGCAGCTCATCACGCGCGGCGATCGGCCGCTCGATGCAGAGGCGGTCGAACGCTATCGTGCGC
TCGAAGCGCGCCGCGCGGCCGGCGAGCCCGTCGCGCAGCTCGTCGGGATGCGCGAGTTCTTCGGCCGCCCGTTCGACGTG
ACGCCCGACGTGCTGATCCCGCGCCCCGAAACCGAACTGCTCGTCGAAGCAGCGCTCGACGCGATCGACGGGCTCGCGCA
TCCGGCCGTGCTCGATCTCGGCACCGGCAGCGGCGCGATCGCGGTGTCGATCGCGGCCGAGCGGCCCGACGCGCGCGTAT
GGGCGCTCGACCGGTCGGCCGCCGCGCTCGCGGTCGCGCAGCGCAACGCCGACAAACTGCTCGATGCGCGCCGCCCCGGC
GGCCCGCTGCAGTGGCTGCAAAGCGACTGGTACGCGGCACTCGATCCCGCGCTCGCCTTCGACGCGATCGTCAGCAATCC
GCCGTATATCGCACAGCACGATCCGCATCTGTCGCAAGGCGACCTGCGCTTCGAGCCGCGCGGCGCGCTGACCGACGACG
CAGACGGGCTGAGCGCGATCCGCACGATCGTCGCCGGTGCGCGTGCGCACCTGAAGCCGGGCGGCACGCTGTGGATCGAG
CACGGCTACGATCAGGCCGAAGCGGTGCGCGCGCTGCTCGCCTCGCACGGTTTCGACGCGGTCGAATCGCTCGCCGATCT
CGCCGCGATCGAACGCACGACGGGCGGCCGCCTGCCCCGCTGA

Upstream 100 bases:

>100_bases
ATCATGGACGGCGATCTCGACGAACTGATCGGCGCGCTCGTCAGCGAGCATCAGGCCGAGCTGCTCGCGTCGCTCGGCGA
CGCCGACTGAGGCCGCGACG

Downstream 100 bases:

>100_bases
CGCCCGGCCCGCCCGGTTGAAATCCGCTATCATTTCGATCTAACGCCCCGCAAACGCAAGGTCAGTCATGGACACCCAAC
AACGTATCAAGCAAATCGTC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSAPTADDLLRATPLDAVDARVLLAHALGWTRTQLITRGDRPLDAEAVERYRALEARRAAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLAHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSAAALAVAQRNADKLLDARRPG
GPLQWLQSDWYAALDPALAFDAIVSNPPYIAQHDPHLSQGDLRFEPRGALTDDADGLSAIRTIVAGARAHLKPGGTLWIE
HGYDQAEAVRALLASHGFDAVESLADLAAIERTTGGRLPR

Sequences:

>Translated_280_residues
MSAPTADDLLRATPLDAVDARVLLAHALGWTRTQLITRGDRPLDAEAVERYRALEARRAAGEPVAQLVGMREFFGRPFDV
TPDVLIPRPETELLVEAALDAIDGLAHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSAAALAVAQRNADKLLDARRPG
GPLQWLQSDWYAALDPALAFDAIVSNPPYIAQHDPHLSQGDLRFEPRGALTDDADGLSAIRTIVAGARAHLKPGGTLWIE
HGYDQAEAVRALLASHGFDAVESLADLAAIERTTGGRLPR
>Mature_279_residues
SAPTADDLLRATPLDAVDARVLLAHALGWTRTQLITRGDRPLDAEAVERYRALEARRAAGEPVAQLVGMREFFGRPFDVT
PDVLIPRPETELLVEAALDAIDGLAHPAVLDLGTGSGAIAVSIAAERPDARVWALDRSAAALAVAQRNADKLLDARRPGG
PLQWLQSDWYAALDPALAFDAIVSNPPYIAQHDPHLSQGDLRFEPRGALTDDADGLSAIRTIVAGARAHLKPGGTLWIEH
GYDQAEAVRALLASHGFDAVESLADLAAIERTTGGRLPR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=287, Percent_Identity=30.3135888501742, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI1787463, Length=260, Percent_Identity=45.7692307692308, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI87082085, Length=199, Percent_Identity=34.6733668341709, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582226, Length=206, Percent_Identity=33.495145631068, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29907; Mature: 29776

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTADDLLRATPLDAVDARVLLAHALGWTRTQLITRGDRPLDAEAVERYRALEARRAA
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLAHPAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEE
AVSIAAERPDARVWALDRSAAALAVAQRNADKLLDARRPGGPLQWLQSDWYAALDPALAF
EEEEECCCCCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHH
DAIVSNPPYIAQHDPHLSQGDLRFEPRGALTDDADGLSAIRTIVAGARAHLKPGGTLWIE
HHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
HGYDQAEAVRALLASHGFDAVESLADLAAIERTTGGRLPR
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAPTADDLLRATPLDAVDARVLLAHALGWTRTQLITRGDRPLDAEAVERYRALEARRAA
CCCCHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GEPVAQLVGMREFFGRPFDVTPDVLIPRPETELLVEAALDAIDGLAHPAVLDLGTGSGAI
CCHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEE
AVSIAAERPDARVWALDRSAAALAVAQRNADKLLDARRPGGPLQWLQSDWYAALDPALAF
EEEEECCCCCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHH
DAIVSNPPYIAQHDPHLSQGDLRFEPRGALTDDADGLSAIRTIVAGARAHLKPGGTLWIE
HHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
HGYDQAEAVRALLASHGFDAVESLADLAAIERTTGGRLPR
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]