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The map label for this gene is pcaF [H]

Identifier: 161526021

GI number: 161526021

Start: 3116710

End: 3117912

Strand: Direct

Name: pcaF [H]

Synonym: Bmul_2852

Alternate gene names: 161526021

Gene position: 3116710-3117912 (Clockwise)

Preceding gene: 161526020

Following gene: 161526022

Centisome position: 90.38

GC content: 68.91

Gene sequence:

>1203_bases
ATGACTGACGCCTATATCTGCGATGCCATTCGCACCCCTATCGGCCGCTACGGCGGCGCCCTGAAAGACGTCCGCGCGGA
CGACCTCGGCGCGGTGCCGCTGAAGGCGCTCGTCGAACGCAACCGCGACGTCGACTGGAACGCGATCGACGACGTGATTT
ACGGCTGCGCGAACCAGGCCGGCGAAGACAACCGCAACGTCGCACGCATGTCGGCGCTGCTGGCCGGGCTGCCGACCGCC
GTCCCCGGCACGACGCTGAACCGGCTCTGCGGCTCGGGCATGGACGCGATCGGCACGGCCGCGCGCGCGATCAAGGCCGG
CGAAGCGCGGCTGATGATCGCCGGCGGCGTCGAAAGCATGACGCGCGCGCCGTTCGTGATGGGCAAGGCCGCAAGCGCAT
TCGCGCGTCAGGCCGACATCTACGACACGACGATCGGCTGGCGTTTCGTCAATCCGCTGATGAAACAGCAATACGGCGTC
GACTCGATGCCGGAGACGGCCGAGAACGTCGCGGTCGACTACAACGTGAGCCGCGCCGATCAGGATCTGTTCGCGCTGCG
CAGCCAGCAGAAGGCGGCCCGCGCGCAGCAGGACGGCACACTGGCCGAGGAAATCGTGCCCGTCACGATTGCGCAGAAGA
AGGGCGACCCGCTCGTCGTGTCGCGCGACGAGCATCCGCGCGAGACCTCGCTCGAAGCACTCGCGAAACTGAAAGGCGTC
GTGCGCGCGGACGGCACGGTGACGGCCGGCAACGCATCGGGCGTCAACGACGGCGCGTGCGCGCTGCTGCTCGCGAATGC
CGAAGCGGCCGACCAGTACGGCCTGCGCCGCCGCGCGCGCGTCATCGGCATGGCGACCGCCGGCGTCGCGCCGCGCGTGA
TGGGCATCGGCCCGGCGCCGGCGACGCAGAAGCTGCTCGCGCGCCTCGGGATGACGATCGACCAGTTCGACGTGATCGAA
CTGAACGAAGCGTTCGCGTCGCAGGGTCTCGCGGTGCTGCGCATGCTCGGTGTTGCCGACGACGATCCTCGCGTGAACCC
GAACGGCGGCGCGATCGCGCTCGGCCATCCGCTCGGCGCGTCGGGCGCGCGGCTCGTGACGACGGCGATGTATCAACTGC
ATCGCACGAACGGCCGTTTCGCGCTGTGCACGATGTGCATCGGCGTCGGGCAAGGCATCGCGCTCGCGATCGAACGCATA
TAA

Upstream 100 bases:

>100_bases
GCCAACCGGCATAACCTGAATGGCGGACGTGCAAACGCCGCCACATTCTCTACCATGAACGAATCCGCCATCCGGCGGGT
TCGTCGCCTTCCGATACGCC

Downstream 100 bases:

>100_bases
CGCACCGCCGCGCGCGCACCTATAACGAATCATCACGGAGACACTGCATGTCCTATCAGGCAATTCGGCTGGACATCGAC
GAAGCCGCCCGCGTGGCGAC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSALLAGLPTA
VPGTTLNRLCGSGMDAIGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQQYGV
DSMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVPVTIAQKKGDPLVVSRDEHPRETSLEALAKLKGV
VRADGTVTAGNASGVNDGACALLLANAEAADQYGLRRRARVIGMATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIALAIERI

Sequences:

>Translated_400_residues
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSALLAGLPTA
VPGTTLNRLCGSGMDAIGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQQYGV
DSMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVPVTIAQKKGDPLVVSRDEHPRETSLEALAKLKGV
VRADGTVTAGNASGVNDGACALLLANAEAADQYGLRRRARVIGMATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIALAIERI
>Mature_399_residues
TDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQAGEDNRNVARMSALLAGLPTAV
PGTTLNRLCGSGMDAIGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQQYGVD
SMPETAENVAVDYNVSRADQDLFALRSQQKAARAQQDGTLAEEIVPVTIAQKKGDPLVVSRDEHPRETSLEALAKLKGVV
RADGTVTAGNASGVNDGACALLLANAEAADQYGLRRRARVIGMATAGVAPRVMGIGPAPATQKLLARLGMTIDQFDVIEL
NEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIALAIERI

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=40.5063291139241, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.0352644836272, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI4501853, Length=406, Percent_Identity=38.423645320197, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=33.9950372208437, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.615925058548, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI194353979, Length=254, Percent_Identity=31.496062992126, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.5785536159601, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.6384039900249, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.1612903225806, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=41.6256157635468, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=31.219512195122, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=42.2110552763819, Blast_Score=300, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI25147385, Length=403, Percent_Identity=34.2431761786601, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=410, Percent_Identity=33.9024390243902, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.3255813953488, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=412, Percent_Identity=30.0970873786408, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=36.6995073891626, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=36.1858190709046, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=42.643391521197, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=37.9652605459057, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=34.6534653465347, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.6037735849057, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42073; Mature: 41941

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQA
CCCHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTAVPGTTLNRLCGSGMDAIGTAARAIKAGEARLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQQYGVDSMPETAENVAVDYNVSRAD
HHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCH
QDLFALRSQQKAARAQQDGTLAEEIVPVTIAQKKGDPLVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHH
VRADGTVTAGNASGVNDGACALLLANAEAADQYGLRRRARVIGMATAGVAPRVMGIGPAP
HCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCC
ATQKLLARLGMTIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIALAIERI
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALVERNRDVDWNAIDDVIYGCANQA
CCHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTAVPGTTLNRLCGSGMDAIGTAARAIKAGEARLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQQYGVDSMPETAENVAVDYNVSRAD
HHCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCCCH
QDLFALRSQQKAARAQQDGTLAEEIVPVTIAQKKGDPLVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHH
VRADGTVTAGNASGVNDGACALLLANAEAADQYGLRRRARVIGMATAGVAPRVMGIGPAP
HCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCC
ATQKLLARLGMTIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLHRTNGRFALCTMCIGVGQGIALAIERI
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]