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The map label for this gene is rocA [H]

Identifier: 161526020

GI number: 161526020

Start: 3114917

End: 3116623

Strand: Direct

Name: rocA [H]

Synonym: Bmul_2851

Alternate gene names: 161526020

Gene position: 3114917-3116623 (Clockwise)

Preceding gene: 161526016

Following gene: 161526021

Centisome position: 90.33

GC content: 67.66

Gene sequence:

>1707_bases
ATGACCCATGCACTGTTCACGAAGCACGAAGACACGCTGAAACGCGCACTCGCCGCCATCGAGAGCCGCGGATACTGGAG
CCCGTTCGCCGAAATGCCGAGCCCCAAAGTGTACGGGGAAAGCGGCAATGCAGACGGCGAAGCCGCGTTCAAATCGCACC
TCGGCAAGACCTTCGAGCTCGACCAGCCGGCCAGCGGCGAGACGGTCGGCGCGGAACGTTCTCCGTATGGCGTCGCGCTC
GACATCCGGTATCCGAAGTCGACGCCCGATGCGCTGATCGCAGCCGCCGCCGCTGCCCAACGCACGTGGCGCGAAGCCGG
CCCGAGCGCGTGGATCGGTGTCAGCCTCGAGATCCTCGCGCGCCTGAACCGCGCCAGTTTCGAAATCGCGTACAGCGTGA
TGCATACGACGGGCCAGGCATTCATGATGGCGTTCCAGGCCGGCGGCCCGCATGCGCAGGACCGCGCGCTCGAGGCCGTC
GCCTATGCGTGGGACCAGCTGCGCCGCATCCCGGCCGACGCGCATTGGGAAAAGCCGCAAGGCAAGAACCCGCCGCTCGC
GATGCAGAAGCGTTACACGATCGTCCCGCGCGGCACCGGGCTCGTGCTCGGCTGCTGCACGTTCCCGACCTGGAACGGTT
ATCCCGGCCTGTTCGCCGATCTCGCGACCGGCAACACGGTCATCGTCAAGCCTCATCCGGGCGCGATCCTGCCGCTCGCG
ATCACCGTGCGGATCGCGCGCGACGTGCTGCGCGAGGCCGGCTTCGATCCTAACGTCGTGACGCTGCTCGCCACGGAGCC
GAACGACGGCGCACTCGTGCAGGACCTCGCGCTGCGCCCCGAGATCAAGCTGATCGACTTCACCGGCAGCACGCAGAACG
GCACGTGGCTCGAACGTCACGCCCATCAGGCGCAGGTCTACACCGAGAAGGCAGGCGTCAATCAGATCGTGATCGACTCG
ACCGACGATCTGAAAGCCGCCGCCAAGAACATCGCGTTCTCGCTCGCGCTGTACTCGGGGCAGATGTGCACCGCCCCGCA
GAACATCTATGTGCCGCGCGACGGCATCCGCACCGCCGACGGCCATGCGAGCTTCGACGAAGTCGCGCAGGCGATCGCCG
GCGCAGTGCAGAAACTCACCGGCGACCCGGCACGGTCGGTCGAGCTGATCGGCGCCATCCAGAACGACGGCGTGACGGCA
CGCATCGACGCCGCGCGCGCAGTCGGCCGCGTGCTCCTCGATAGTCAGACGCTTCAGCACCCCGCCTTCCCCGACGCTCG
CGTGCGCACGCCGCTCGTGCTGCAGCTCGACGTCGCCGACCGCGAAAAATTCACGCAGGAATGGTTCGGGCCGATCTCGT
TCGTGATCGCGACCGACTCGACCGCGCAGTCGCTCGATCTCGCCGGCGAGATCGCCGCCGAGCACGGCGCGCTGACGCTG
TCCGTCTACAGCACGGCCGACGACGTGATCGACGCCGCACACGAAGCCGCGGTGCGCGGCGGCGTCGCACTGTCGATCAA
CCTGACGGGCGGCGTGTTCGTCAATCAGTCGGCGGCGTTCTCTGACTTCCACGGTACGGGCGCCAATCCGGCCGCGAACG
CTGCGCTGGCCGATGCCGCATTCGTCGCCAACCGCTTCCGCGTGGTTCAGAGCCGCGTCCATGTTGCGCCGAAGGCGGCG
CCCGCGGAAGTCGGCCAACCGGCATAA

Upstream 100 bases:

>100_bases
AACGCCTCGCACACGCGAATAGTTTGATGCTACCATTCTCCGACCAAGCGGTCGGTTAATTAATCGATCCAACCCTTCTC
ACGTTCATCAGGCCGCCGTC

Downstream 100 bases:

>100_bases
CCTGAATGGCGGACGTGCAAACGCCGCCACATTCTCTACCATGAACGAATCCGCCATCCGGCGGGTTCGTCGCCTTCCGA
TACGCCATGACTGACGCCTA

Product: phenylacetic acid degradation protein paaN

Products: NA

Alternate protein names: P5C dehydrogenase [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTHALFTKHEDTLKRALAAIESRGYWSPFAEMPSPKVYGESGNADGEAAFKSHLGKTFELDQPASGETVGAERSPYGVAL
DIRYPKSTPDALIAAAAAAQRTWREAGPSAWIGVSLEILARLNRASFEIAYSVMHTTGQAFMMAFQAGGPHAQDRALEAV
AYAWDQLRRIPADAHWEKPQGKNPPLAMQKRYTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
ITVRIARDVLREAGFDPNVVTLLATEPNDGALVQDLALRPEIKLIDFTGSTQNGTWLERHAHQAQVYTEKAGVNQIVIDS
TDDLKAAAKNIAFSLALYSGQMCTAPQNIYVPRDGIRTADGHASFDEVAQAIAGAVQKLTGDPARSVELIGAIQNDGVTA
RIDAARAVGRVLLDSQTLQHPAFPDARVRTPLVLQLDVADREKFTQEWFGPISFVIATDSTAQSLDLAGEIAAEHGALTL
SVYSTADDVIDAAHEAAVRGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVAPKAA
PAEVGQPA

Sequences:

>Translated_568_residues
MTHALFTKHEDTLKRALAAIESRGYWSPFAEMPSPKVYGESGNADGEAAFKSHLGKTFELDQPASGETVGAERSPYGVAL
DIRYPKSTPDALIAAAAAAQRTWREAGPSAWIGVSLEILARLNRASFEIAYSVMHTTGQAFMMAFQAGGPHAQDRALEAV
AYAWDQLRRIPADAHWEKPQGKNPPLAMQKRYTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
ITVRIARDVLREAGFDPNVVTLLATEPNDGALVQDLALRPEIKLIDFTGSTQNGTWLERHAHQAQVYTEKAGVNQIVIDS
TDDLKAAAKNIAFSLALYSGQMCTAPQNIYVPRDGIRTADGHASFDEVAQAIAGAVQKLTGDPARSVELIGAIQNDGVTA
RIDAARAVGRVLLDSQTLQHPAFPDARVRTPLVLQLDVADREKFTQEWFGPISFVIATDSTAQSLDLAGEIAAEHGALTL
SVYSTADDVIDAAHEAAVRGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVAPKAA
PAEVGQPA
>Mature_567_residues
THALFTKHEDTLKRALAAIESRGYWSPFAEMPSPKVYGESGNADGEAAFKSHLGKTFELDQPASGETVGAERSPYGVALD
IRYPKSTPDALIAAAAAAQRTWREAGPSAWIGVSLEILARLNRASFEIAYSVMHTTGQAFMMAFQAGGPHAQDRALEAVA
YAWDQLRRIPADAHWEKPQGKNPPLAMQKRYTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLAI
TVRIARDVLREAGFDPNVVTLLATEPNDGALVQDLALRPEIKLIDFTGSTQNGTWLERHAHQAQVYTEKAGVNQIVIDST
DDLKAAAKNIAFSLALYSGQMCTAPQNIYVPRDGIRTADGHASFDEVAQAIAGAVQKLTGDPARSVELIGAIQNDGVTAR
IDAARAVGRVLLDSQTLQHPAFPDARVRTPLVLQLDVADREKFTQEWFGPISFVIATDSTAQSLDLAGEIAAEHGALTLS
VYSTADDVIDAAHEAAVRGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAAFVANRFRVVQSRVHVAPKAAP
AEVGQPA

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldehyde dehydrogenase family. RocA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787250, Length=367, Percent_Identity=27.2479564032698, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320917, Length=309, Percent_Identity=28.8025889967638, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323822, Length=428, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323821, Length=428, Percent_Identity=25.2336448598131, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666674, Length=292, Percent_Identity=27.0547945205479, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016160
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR005932 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.5.1.12 [H]

Molecular weight: Translated: 60422; Mature: 60290

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHALFTKHEDTLKRALAAIESRGYWSPFAEMPSPKVYGESGNADGEAAFKSHLGKTFEL
CCCCEECCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCCHHHHHHHCCCEEEC
DQPASGETVGAERSPYGVALDIRYPKSTPDALIAAAAAAQRTWREAGPSAWIGVSLEILA
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
RLNRASFEIAYSVMHTTGQAFMMAFQAGGPHAQDRALEAVAYAWDQLRRIPADAHWEKPQ
HHCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GKNPPLAMQKRYTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHEECCCCCEEEECCCCCCEEHHH
ITVRIARDVLREAGFDPNVVTLLATEPNDGALVQDLALRPEIKLIDFTGSTQNGTWLERH
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHH
AHQAQVYTEKAGVNQIVIDSTDDLKAAAKNIAFSLALYSGQMCTAPQNIYVPRDGIRTAD
HHHHHEEHHHCCCCEEEEECCHHHHHHHHHHEEEEEEECCCEECCCCCEEECCCCCCCCC
GHASFDEVAQAIAGAVQKLTGDPARSVELIGAIQNDGVTARIDAARAVGRVLLDSQTLQH
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHCCHHHCC
PAFPDARVRTPLVLQLDVADREKFTQEWFGPISFVIATDSTAQSLDLAGEIAAEHGALTL
CCCCCCCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
SVYSTADDVIDAAHEAAVRGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAA
EEECCHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
FVANRFRVVQSRVHVAPKAAPAEVGQPA
HHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
THALFTKHEDTLKRALAAIESRGYWSPFAEMPSPKVYGESGNADGEAAFKSHLGKTFEL
CCCEECCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCCHHHHHHHCCCEEEC
DQPASGETVGAERSPYGVALDIRYPKSTPDALIAAAAAAQRTWREAGPSAWIGVSLEILA
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
RLNRASFEIAYSVMHTTGQAFMMAFQAGGPHAQDRALEAVAYAWDQLRRIPADAHWEKPQ
HHCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GKNPPLAMQKRYTIVPRGTGLVLGCCTFPTWNGYPGLFADLATGNTVIVKPHPGAILPLA
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHEECCCCCEEEECCCCCCEEHHH
ITVRIARDVLREAGFDPNVVTLLATEPNDGALVQDLALRPEIKLIDFTGSTQNGTWLERH
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHH
AHQAQVYTEKAGVNQIVIDSTDDLKAAAKNIAFSLALYSGQMCTAPQNIYVPRDGIRTAD
HHHHHEEHHHCCCCEEEEECCHHHHHHHHHHEEEEEEECCCEECCCCCEEECCCCCCCCC
GHASFDEVAQAIAGAVQKLTGDPARSVELIGAIQNDGVTARIDAARAVGRVLLDSQTLQH
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHCCHHHCC
PAFPDARVRTPLVLQLDVADREKFTQEWFGPISFVIATDSTAQSLDLAGEIAAEHGALTL
CCCCCCCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
SVYSTADDVIDAAHEAAVRGGVALSINLTGGVFVNQSAAFSDFHGTGANPAANAALADAA
EEECCHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
FVANRFRVVQSRVHVAPKAAPAEVGQPA
HHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA