BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is paaG [H]

Identifier: 161526022

GI number: 161526022

Start: 3117960

End: 3118751

Strand: Direct

Name: paaG [H]

Synonym: Bmul_2853

Alternate gene names: 161526022

Gene position: 3117960-3118751 (Clockwise)

Preceding gene: 161526021

Following gene: 161526023

Centisome position: 90.42

GC content: 68.18

Gene sequence:

>792_bases
ATGTCCTATCAGGCAATTCGGCTGGACATCGACGAAGCCGCCCGCGTGGCGACGATCACGCTCGATCGTCCCGACAAGCT
GAACAGCTTTACGCGCGCGATGCATCGCGAACTGCAGTCGGCGCTCGACGAAGTCGGCGCGGCCGGTGCGCGCGCACTGA
TTCTGACGGGTGCGGGGCGCGGATTCTGCGCCGGTCAGGATCTCGCGGACCTCGACTTCACGCCGGGCGCGTCGACCGAC
CTCGGCGCGCTGATCGACGAGCACTTCAATCCGCTGATCCGGCGCCTGCAGCGCATGCCGATTCCGGTGATCGCCGCCGT
CAACGGTACGGCCGCCGGTGCGGGCGCGAATCTCGCGCTCGCGTGCGATATCGTGCTCGCCGCACGCTCGAGCAGCTTCA
TCCAGGCTTTCGTCAAGATCGGCCTCGTGCCGGATTCGGGCGGCACGTGGTTCCTGCCGCAGCGCGTCGGGATGGCGCGC
GCGCTGGGTCTTGCGCTGACCGGCGACAAGCTCGGCGCCGAACAGGCGGAACAGTGGGGACTGATCTGGCGCGCCGTCGA
CGACGACGCGCTCGCCGCATCGGCCCGTGAGCTGGCCACGCGGCTCGCGCAGCAACCGACGCTCGCGATCGCGTCGATCA
AGCAGGCGATGCGCGACGGCATGACCCATACGCTCGACCAGCAACTCGATCTGGAGCGCGACCTGCAGCGCAAGCTCGGC
CAGTCGCACGATTACGCCGAAGGCGTCAACGCATTCATCGAAAAGCGCGCTCCGCGCTTCGAGGGCCGCTGA

Upstream 100 bases:

>100_bases
CGATGTGCATCGGCGTCGGGCAAGGCATCGCGCTCGCGATCGAACGCATATAACGCACCGCCGCGCGCGCACCTATAACG
AATCATCACGGAGACACTGC

Downstream 100 bases:

>100_bases
CATGACGACGAACCCGACGACCGACACGCTCGATCCCGACGCTCTCGCACGCATGACCGCGCAGACCATGTACGAAGCCG
ACGCGTGCAGCCGCGCGCTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSYQAIRLDIDEAARVATITLDRPDKLNSFTRAMHRELQSALDEVGAAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGALIDEHFNPLIRRLQRMPIPVIAAVNGTAAGAGANLALACDIVLAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDALAASARELATRLAQQPTLAIASIKQAMRDGMTHTLDQQLDLERDLQRKLG
QSHDYAEGVNAFIEKRAPRFEGR

Sequences:

>Translated_263_residues
MSYQAIRLDIDEAARVATITLDRPDKLNSFTRAMHRELQSALDEVGAAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGALIDEHFNPLIRRLQRMPIPVIAAVNGTAAGAGANLALACDIVLAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDALAASARELATRLAQQPTLAIASIKQAMRDGMTHTLDQQLDLERDLQRKLG
QSHDYAEGVNAFIEKRAPRFEGR
>Mature_262_residues
SYQAIRLDIDEAARVATITLDRPDKLNSFTRAMHRELQSALDEVGAAGARALILTGAGRGFCAGQDLADLDFTPGASTDL
GALIDEHFNPLIRRLQRMPIPVIAAVNGTAAGAGANLALACDIVLAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARA
LGLALTGDKLGAEQAEQWGLIWRAVDDDALAASARELATRLAQQPTLAIASIKQAMRDGMTHTLDQQLDLERDLQRKLGQ
SHDYAEGVNAFIEKRAPRFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=33.7301587301587, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=29.5880149812734, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=29.6442687747036, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=30.6201550387597, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221136756, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221136753, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221307494, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=27.2321428571429, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=27.2321428571429, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=28.4916201117318, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=27.9620853080569, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=27.9620853080569, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=211, Percent_Identity=27.9620853080569, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=29.6875, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI25453481, Length=243, Percent_Identity=23.4567901234568, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI51538573, Length=243, Percent_Identity=23.4567901234568, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4757968, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=237, Percent_Identity=23.6286919831224, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538581, Length=237, Percent_Identity=23.6286919831224, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI116235487, Length=203, Percent_Identity=26.1083743842365, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI157694520, Length=205, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=57.2, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=35.408560311284, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=36.2962962962963, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=28.7313432835821, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=28.735632183908, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=30.6930693069307, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=34.5381526104418, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=31.2977099236641, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=29.2592592592593, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=26.1224489795918, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=224, Percent_Identity=26.3392857142857, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=24.904214559387, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=30.2040816326531, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=26.2222222222222, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=234, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=232, Percent_Identity=25.4310344827586, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=26.9607843137255, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=31.8007662835249, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=31.8007662835249, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=30.7086614173228, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24654903, Length=265, Percent_Identity=24.9056603773585, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=26.2548262548263, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.8039215686275, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=204, Percent_Identity=28.4313725490196, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28200; Mature: 28069

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQAIRLDIDEAARVATITLDRPDKLNSFTRAMHRELQSALDEVGAAGARALILTGAGR
CCCEEEEEEHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GFCAGQDLADLDFTPGASTDLGALIDEHFNPLIRRLQRMPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEH
ACDIVLAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCHHCHHHHHHHC
LIWRAVDDDALAASARELATRLAQQPTLAIASIKQAMRDGMTHTLDQQLDLERDLQRKLG
EEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHCC
QSHDYAEGVNAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYQAIRLDIDEAARVATITLDRPDKLNSFTRAMHRELQSALDEVGAAGARALILTGAGR
CCEEEEEEHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GFCAGQDLADLDFTPGASTDLGALIDEHFNPLIRRLQRMPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEH
ACDIVLAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCHHCHHHHHHHC
LIWRAVDDDALAASARELATRLAQQPTLAIASIKQAMRDGMTHTLDQQLDLERDLQRKLG
EEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHCC
QSHDYAEGVNAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]