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The map label for this gene is folD [H]

Identifier: 161524305

GI number: 161524305

Start: 1243027

End: 1243884

Strand: Reverse

Name: folD [H]

Synonym: Bmul_1130

Alternate gene names: 161524305

Gene position: 1243884-1243027 (Counterclockwise)

Preceding gene: 161524306

Following gene: 161524304

Centisome position: 36.07

GC content: 67.72

Gene sequence:

>858_bases
ATGACAGCCCTCCTCATCGACGGCAACGCCCTCTCGAAGACCTTGCGCGCGCAGGCCGCCGAACGCGCCGCCGCCCTGAC
CGCCCGCGGCCACCGGCCCGGCCTCGCGGTGATCCTCGTCGGCGACAACCCGGCGAGCGAAGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCAGAAGGACACGTACCCGGCGACGCTGTCCGAGGCCGACCTGCTCGCG
CGCATCGACGAACTGAACCGCGATCCGAAGATTCACGGGATCCTCGTGCAACTGCCGCTGCCCGCGCATATCGATAGCCA
CAAGGTGATCGAGGCGATCGCGCCCGAGAAGGACGTCGACGGCTTTCATGTCGCCAACGCCGGCGCGCTGATGACGGGCA
AGCCGCTGTTCCGCCCGTGCACGCCGTACGGCGTGATGAAGATGTTCGCCGCGCACGGCATTGCGCTGCAGGGTGCGAAC
GCGGTCGTGATCGGCCGCTCGAACATCGTCGGCAAGCCGATGGCGATGCTGCTGCTCGAAGCGGGCGCAACCGTGACGAT
CTGCCACAGCAAGACGCGCGACCTCGCCGCCCATACGCGGCAGGCCGACATCATCGTCGCCGCGGTCGGCAAGCGCAACA
TCCTGACCGCCGACATGGTGAAGCCGGGCGCGACCGTGATCGACGTCGGAATGAACCGCGACGACGCAGGCAAGCTGTGC
GGCGACGTCGATTTCGCGGGCGTGAAGGAAGTCGCCGGCTACATCACGCCGGTGCCGGGCGGCGTCGGCCCGATGACGAT
CACGATGCTGCTGATCAACACGCTCGAATCGGCCGAGCGCGCCGCCGCGGCTGCCTGA

Upstream 100 bases:

>100_bases
TGTCGGACCGCGACAGGCGAACGGTATAATTGCGTGCTTTGCTGCGGCGTTTCCCGATGCCGCACGCCCGCATTCCAACT
TCCCGGCAGAACCCACCACC

Downstream 100 bases:

>100_bases
CCCCATGCCCGCCGGCCGCCGCTCGGCGGCCTCGCGCGAATGCCGCTCGCTGCTCGCCGCGCGCCCTCGCGCATCGCATG
CGCGCCGCGCATCCGACCCT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSQKDTYPATLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFAAHGIALQGAN
AVVIGRSNIVGKPMAMLLLEAGATVTICHSKTRDLAAHTRQADIIVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLESAERAAAAA

Sequences:

>Translated_285_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSQKDTYPATLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFAAHGIALQGAN
AVVIGRSNIVGKPMAMLLLEAGATVTICHSKTRDLAAHTRQADIIVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLESAERAAAAA
>Mature_284_residues
TALLIDGNALSKTLRAQAAERAAALTARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSQKDTYPATLSEADLLAR
IDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFAAHGIALQGANA
VVIGRSNIVGKPMAMLLLEAGATVTICHSKTRDLAAHTRQADIIVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLCG
DVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLESAERAAAAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=47.2789115646259, Blast_Score=241, Evalue=4e-64,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=47.2789115646259, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.7586206896552, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=27.3542600896861, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=52.6881720430108, Blast_Score=283, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=43.0508474576271, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=47.2027972027972, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=41.6666666666667, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=294, Percent_Identity=25.8503401360544, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.2993197278912, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.2993197278912, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=45.6747404844291, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=45.6747404844291, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=45.6747404844291, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=45.6747404844291, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29855; Mature: 29724

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALLIDGNALSKTLRAQAAERAAALTARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCC
GFFSQKDTYPATLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFAAHGIALQGANAVVIGRSNIVGKPMAMLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCCCCCHHHHHHHH
AGATVTICHSKTRDLAAHTRQADIIVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCEEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLESAERAAAAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALLIDGNALSKTLRAQAAERAAALTARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCC
GFFSQKDTYPATLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFAAHGIALQGANAVVIGRSNIVGKPMAMLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCCCCCHHHHHHHH
AGATVTICHSKTRDLAAHTRQADIIVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCEEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTLESAERAAAAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA