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The map label for this gene is cysD [H]

Identifier: 161524001

GI number: 161524001

Start: 899105

End: 900067

Strand: Direct

Name: cysD [H]

Synonym: Bmul_0822

Alternate gene names: 161524001

Gene position: 899105-900067 (Clockwise)

Preceding gene: 161524000

Following gene: 161524002

Centisome position: 26.07

GC content: 66.77

Gene sequence:

>963_bases
ATGAGCACGACGCTCGAGCAATCCGCCTTTGCCCCGCCCACCGGCGCCGACAGCCGCATGGGCCATCTCGACTGGCTCGA
AGCCGAGTCGATCCATATCCTGCGCGAGCTGGTCGCCGAGTGCAGCAAGCCGGCGCTGTTGTTCTCGGGCGGCAAGGATT
CGGTCGTCGTGCTGCACCTCGCGCTGAAGGCGTTCGGTCTCGGCGCGAACCGCAAGACGACGCTGCCGTTCCCGCTCGTC
CATATCGACACCGGCCACAACTACGAAGAAGTGATCGACTTCCGCGATCGCCGTGCGAAGGAGATCGGCGCCGAGCTGAT
CGTCGGCCACGTCGAGGACTCGATCAAGCGCGGCACGGTCGTGCTGCGCCGCGAGACCGATTCGCGCAATGCCGCGCAGG
CCGTCACGCTGCTCGAGACGATCGACGCGCACGGCTTCACCGCGATGATCGGCGGTGCGCGCCGCGACGAGGAGAAGGCG
CGCGCGAAGGAGCGCATCTTCTCGTTCCGCGACGAATTCGGCCAGTGGGATCCGAAGGCGCAGCGTCCGGAACTGTGGAG
CCTGTACAACGCGCGCCTGCACAAGGGCGAGCATCTGCGCGTGTTCCCGATCTCGAACTGGACCGAGCTCGACGTGTGGC
AGTACATCGCGCGCGAGAACCTCGAGCTGCCGTCGATCTACTACGCGCACCAGCGCGAGATCGTCCGCCGCAACGGCCTG
CTCGTCCCCGTCACGCCGCTCACGCCGATGCGCGAGGGCGAGACGAGCGAGATCGCGCAGGTGCGGTTCCGCACGGTCGG
CGACATTTCCTGCACGTGCCCGGTCGAGAGCGACGCCGACGACGTCGAGAAGATCATCGCCGAGACGGCGGTGACCGAGA
TCACCGAGCGCGGCGCGACGCGGATGGACGACCAGACGTCGGAAGCCGCGATGGAACAGCGCAAGAAGCAAGGCTATTTC
TGA

Upstream 100 bases:

>100_bases
CAAGCGCCGACGCATCGCACTGAAGCACGAACGACAAGATCGAATATTGCGCCGCTCGCGACAGCGGGCGGCACGAACCC
AGAAGAGAAGGACCGAAACC

Downstream 100 bases:

>100_bases
CGCACACGAGGACATTCACATCATGAGCATCATCGAGAACACCGAAGACCTCGGCGTGCTGCGCTTCATCACCGCAGGCA
GCGTCGACGACGGCAAGAGC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLV
HIDTGHNYEEVIDFRDRRAKEIGAELIVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIDAHGFTAMIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
LVPVTPLTPMREGETSEIAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF

Sequences:

>Translated_320_residues
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLV
HIDTGHNYEEVIDFRDRRAKEIGAELIVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIDAHGFTAMIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
LVPVTPLTPMREGETSEIAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF
>Mature_319_residues
STTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLVH
IDTGHNYEEVIDFRDRRAKEIGAELIVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIDAHGFTAMIGGARRDEEKAR
AKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGLL
VPVTPLTPMREGETSEIAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=47.0394736842105, Blast_Score=272, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36128; Mature: 35997

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHEE
ALKAFGLGANRKTTLPFPLVHIDTGHNYEEVIDFRDRRAKEIGAELIVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VLRRETDSRNAAQAVTLLETIDAHGFTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMREGETSEIAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQTSEAAMEQRKKQGYF
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHEE
ALKAFGLGANRKTTLPFPLVHIDTGHNYEEVIDFRDRRAKEIGAELIVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VLRRETDSRNAAQAVTLLETIDAHGFTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMREGETSEIAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQTSEAAMEQRKKQGYF
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]