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The map label for this gene is cysN [H]

Identifier: 161524002

GI number: 161524002

Start: 900090

End: 901406

Strand: Direct

Name: cysN [H]

Synonym: Bmul_0823

Alternate gene names: 161524002

Gene position: 900090-901406 (Clockwise)

Preceding gene: 161524001

Following gene: 161524003

Centisome position: 26.1

GC content: 67.65

Gene sequence:

>1317_bases
ATGAGCATCATCGAGAACACCGAAGACCTCGGCGTGCTGCGCTTCATCACCGCAGGCAGCGTCGACGACGGCAAGAGCAC
GCTGATCGGCCGCCTGCTGTACGACAGCAAGGCCGTGCTGTCCGACCAGCTGTCCGCGCTGTCGCGTGCGAAGAACAAGC
GCACGGTCGGCGACGAGCTCGACCTCGCGCTGCTGACCGACGGCCTCGAAGCCGAGCGCGAGCAAGGCATCACGATCGAC
GTCGCGTACCGCTACTTCGCGACCGCCAAGCGCAAGTTCATCATCGCCGACACGCCGGGCCACGAGCAGTACACGCGCAA
CATGGTGACGGGCGCGTCGACCGCGCACGCGGCGATCATCCTGATCGACGCGACGCGCGTGACGGTCGAGAACGGCGTTG
CCGAACTGCTGCCGCAGACGAAGCGCCACAGCGCGATCGTGAAGCTGCTCGCGCTGCAGCACGTGATCGTCGCGATCAAC
AAGATGGACCTCGTCGACTACAGCGAAGCGCGCTTCAACGAGATCCGCGACGCGTACGTCGCGCTCGCGAAGCAGCTCGG
GCTGACCGACGTGCGTTTCGTGCCGGTCTCGGCGCTCAAGGGCGACAACATCGTCGGCGCGAGCGAGCGCATGCCGTGGT
ATGCGGGCGAGCCGCTGCTCGACGTGCTCGAGTCGCTGCCGGTCGCGACGCAGGCGCACGACGCGCTGCGCTTCCCGGTG
CAGTGGGTCGCGCGTCAGGACGGCAGCTCGGCCGACGACTTCCGCGGCTACATGGGCCGTATCGAGTCGGGCGAGGTGAA
GGTCGGCGACGAGATCGTCGTGCTGCCGTCGAACCGTCCCGCGACGGTCGCCGAGATCATCGCGCCGGTGCCGGGCGGCA
CCGCGGCCGTGCCGCACGCGTTCGCGGGTCAGACGGTGACGATCCGTCTCGACGAAGACGTCGACGTGTCGCGCGGCGAC
ATGTTCGTGACGGCCGCCGAGCCGGTCGCGCCCGCGAAGAAGCTCGAGGCGGACCTGTGCTGGTTCGACGAGACGCCGCT
GTCGCCGCAGCGCAAGTACCTGCTGAAGCAGACGACGAGCACGGTGTTCGCGAAGATCGGCGGCGTCAAGCAGGTGCTCG
ACGTGCATACGCTGTCGCACGCGACCGACCGTCAGGACCTGAAGATGAACGACATCGGCCGCGTCGCGCTGACGCTGCAA
AAGCCGATCGTGTGCGATACGTACGACGCGCATCCGGGCACGGGCGCGTTCGTGCTGATCGACGAGGCGACGCATCACAC
GGTCGCCGCAGGGATGATCCGGGCGTTTTCGGCGTAA

Upstream 100 bases:

>100_bases
GAGCGCGGCGCGACGCGGATGGACGACCAGACGTCGGAAGCCGCGATGGAACAGCGCAAGAAGCAAGGCTATTTCTGACG
CACACGAGGACATTCACATC

Downstream 100 bases:

>100_bases
CCGGCATCGCGCTGCCGCCACGCGGCGGCGCGCGCCGGGAGCGTCGCCCGCATGAAGGCGAAGCGACAAACATGGGCAAG
GTGTATCTGATCGGCGCAGG

Product: sulfate adenylyltransferase, large subunit

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITID
VAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIILIDATRVTVENGVAELLPQTKRHSAIVKLLALQHVIVAIN
KMDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVATQAHDALRFPV
QWVARQDGSSADDFRGYMGRIESGEVKVGDEIVVLPSNRPATVAEIIAPVPGGTAAVPHAFAGQTVTIRLDEDVDVSRGD
MFVTAAEPVAPAKKLEADLCWFDETPLSPQRKYLLKQTTSTVFAKIGGVKQVLDVHTLSHATDRQDLKMNDIGRVALTLQ
KPIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA

Sequences:

>Translated_438_residues
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITID
VAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIILIDATRVTVENGVAELLPQTKRHSAIVKLLALQHVIVAIN
KMDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVATQAHDALRFPV
QWVARQDGSSADDFRGYMGRIESGEVKVGDEIVVLPSNRPATVAEIIAPVPGGTAAVPHAFAGQTVTIRLDEDVDVSRGD
MFVTAAEPVAPAKKLEADLCWFDETPLSPQRKYLLKQTTSTVFAKIGGVKQVLDVHTLSHATDRQDLKMNDIGRVALTLQ
KPIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA
>Mature_437_residues
SIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITIDV
AYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIILIDATRVTVENGVAELLPQTKRHSAIVKLLALQHVIVAINK
MDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVATQAHDALRFPVQ
WVARQDGSSADDFRGYMGRIESGEVKVGDEIVVLPSNRPATVAEIIAPVPGGTAAVPHAFAGQTVTIRLDEDVDVSRGDM
FVTAAEPVAPAKKLEADLCWFDETPLSPQRKYLLKQTTSTVFAKIGGVKQVLDVHTLSHATDRQDLKMNDIGRVALTLQK
PIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=29.8850574712644, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=29.8850574712644, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194018522, Length=426, Percent_Identity=28.4037558685446, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI194097354, Length=426, Percent_Identity=28.4037558685446, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI194018520, Length=426, Percent_Identity=28.4037558685446, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=27.4157303370787, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI46094014, Length=426, Percent_Identity=27.9342723004695, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.0269058295964, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI34147630, Length=332, Percent_Identity=27.710843373494, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789108, Length=426, Percent_Identity=50.2347417840376, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI2367247, Length=357, Percent_Identity=25.2100840336134, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789737, Length=268, Percent_Identity=29.1044776119403, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=268, Percent_Identity=29.1044776119403, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532067, Length=341, Percent_Identity=34.0175953079179, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI115532065, Length=341, Percent_Identity=34.0175953079179, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=29.372197309417, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=29.372197309417, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=27.5626423690205, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=27.6404494382022, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32566301, Length=144, Percent_Identity=31.9444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=265, Percent_Identity=28.3018867924528, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=341, Percent_Identity=24.633431085044, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322937, Length=364, Percent_Identity=29.9450549450549, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6325337, Length=464, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=464, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=445, Percent_Identity=27.1910112359551, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=269, Percent_Identity=28.996282527881, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=29.490022172949, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=29.490022172949, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=28.6031042128603, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=28.6031042128603, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=28.6031042128603, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=28.6031042128603, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=29.4252873563218, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.3103448275862, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281363316, Length=275, Percent_Identity=30.1818181818182, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864358, Length=275, Percent_Identity=30.1818181818182, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921738, Length=159, Percent_Identity=32.0754716981132, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 47481; Mature: 47349

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEL
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
DLALLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAII
CEEEEECCCHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEE
LIDATRVTVENGVAELLPQTKRHSAIVKLLALQHVIVAINKMDLVDYSEARFNEIRDAYV
EEEEEEEEHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVATQAHDALRFPV
HHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCH
QWVARQDGSSADDFRGYMGRIESGEVKVGDEIVVLPSNRPATVAEIIAPVPGGTAAVPHA
HHHHHCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCHHHHHCCCCCCCCCCCCC
FAGQTVTIRLDEDVDVSRGDMFVTAAEPVAPAKKLEADLCWFDETPLSPQRKYLLKQTTS
CCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHCCCEEECCCCCCCHHHHHHHHHHHH
TVFAKIGGVKQVLDVHTLSHATDRQDLKMNDIGRVALTLQKPIVCDTYDAHPGTGAFVLI
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEE
DEATHHTVAAGMIRAFSA
ECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEL
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
DLALLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAII
CEEEEECCCHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEE
LIDATRVTVENGVAELLPQTKRHSAIVKLLALQHVIVAINKMDLVDYSEARFNEIRDAYV
EEEEEEEEHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVATQAHDALRFPV
HHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCH
QWVARQDGSSADDFRGYMGRIESGEVKVGDEIVVLPSNRPATVAEIIAPVPGGTAAVPHA
HHHHHCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCHHHHHCCCCCCCCCCCCC
FAGQTVTIRLDEDVDVSRGDMFVTAAEPVAPAKKLEADLCWFDETPLSPQRKYLLKQTTS
CCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHCCCEEECCCCCCCHHHHHHHHHHHH
TVFAKIGGVKQVLDVHTLSHATDRQDLKMNDIGRVALTLQKPIVCDTYDAHPGTGAFVLI
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEE
DEATHHTVAAGMIRAFSA
ECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA