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The map label for this gene is pyk [H]

Identifier: 160903346

GI number: 160903346

Start: 2122137

End: 2123573

Strand: Direct

Name: pyk [H]

Synonym: Pmob_1917

Alternate gene names: 160903346

Gene position: 2122137-2123573 (Clockwise)

Preceding gene: 160903345

Following gene: 160903347

Centisome position: 97.81

GC content: 36.74

Gene sequence:

>1437_bases
ATGAACGAAAAAATAGAAAATAAAAAGACGAGAATCGTTTGCACAATTGGTCCAGCTACACAAGATGAAACGATGATAAA
AAAATTGATAAATGCAGGAATGAATGTAGCAAGATTGAATACTTCTCACGATACCATTGCTGATCATGAGAAAAGAGTAA
ATTTAATAAAGAAGATACGAAAAGATTTGAACATACCTTTTGCCATTTTACTCGATTTAGAAGGTCCTAAGATTCGTACG
GGCAAATTTGAGACGGATCAAGTAATGTTGGAAGAGGGACAGAAATTTATTTTGACTATCGAGGAGATCGTTGGCAACAA
AGAAAGGGTGAGTATAAATTATCGAGAATTACCTAAAGAAGTTAAAAAAGGAGATTTTATTCTTCTTGATGATGGAAAAA
TTCGACTTGTAGTCGTTAGCAGTAACGAAAAAGAAATTGTTACGAAGGTTGTAACCGGTGGCAGTATCACTCACAGAAGA
GGGATAAACGTTCCTGGAATAGATATCAGTTTGCCACCTTTAACAGAAAAAGATATGGAGTATTTAAACAAGGCTGTAGA
ATGGAACGTAGATTATATAGCACAATCTTTTGTTAGAAAAGCTGAAGATATCACTAGAACGAGGAGAATTCTAACGGAAT
TGGGTATGCCCGATCTCCCCATTATCGCTAAAATCGAAACATTACAAGCTCTAGACAACCTTGAATCGATCATTGAAGAA
GCTGATGGGGTAATGGTCGCAAGAGGAGATTTGGGTGTTGAAGCACCAGTTGAACAAATACCTTTACTTCAAAAGAGAAT
AATAGAGATTGCAAATACAATGGCAAAGCCTGCTATAACAGCTACCCAAATGCTTGAAAGTATGGTTAACAATCCTTTTC
CTACAAGAGCAGAAGCTACTGATATAGCTAATGCTATATTAGATGGGACGGATGCAGTTATGTTGTCTGAAGAAACGTCG
ATTGGGAAATACCCTGAACAAGCTGTCAAAGTTATGGCTAATGTAGCTAAAGAAACCGAAAAAATACTAGAAGAGTACTA
CTACAAATTTGATTATTCAACATACGGTGGTGGAGATCCCGCTACCAATTCGATAACGATGTCTGCAATAAAAATTGCCG
AGCAATTAGGAATTGATGTTATAGTTGCCACCACTTATAGTGGTTATACGGCAAGAGCTTTGTCTAGGTTCAGAAGAAAT
ATTAAAATAGTTGCTGCATCTCCAAGGATAACTACTTATCATCGATTGGCATTGATATGGGGAGTCACCCCCGTTATCAT
GCAAAAATTTACCGACACAGATAATATGTTGGAGAGCGTTAGCAATATCGTAAAATCTTTAGATTTTGGTGTATCAGGAG
AAAATATCATAGTGACGGCTGGGATTCCCTATGGTTTCTCCAGTAAAACCAATCTCTTAAAGGTTCATGAGATATGA

Upstream 100 bases:

>100_bases
AGGGGTAAACCCCTTGAAAATTCCCAAGGCATGGAATCTAAAAAAAAGATGTAATTTGGGAAGAAACACCCAAAAGTTAC
GCATCTTTAGGAGGATAGTT

Downstream 100 bases:

>100_bases
TATAATTAATACATTAGTAAAAATTTATTTTTGAGGTGATTTTTATGAATAAAATAGATGAATTAACTCCAAAAAAAGTT
GTAGAAAAATTGGATAACTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNEKIENKKTRIVCTIGPATQDETMIKKLINAGMNVARLNTSHDTIADHEKRVNLIKKIRKDLNIPFAILLDLEGPKIRT
GKFETDQVMLEEGQKFILTIEEIVGNKERVSINYRELPKEVKKGDFILLDDGKIRLVVVSSNEKEIVTKVVTGGSITHRR
GINVPGIDISLPPLTEKDMEYLNKAVEWNVDYIAQSFVRKAEDITRTRRILTELGMPDLPIIAKIETLQALDNLESIIEE
ADGVMVARGDLGVEAPVEQIPLLQKRIIEIANTMAKPAITATQMLESMVNNPFPTRAEATDIANAILDGTDAVMLSEETS
IGKYPEQAVKVMANVAKETEKILEEYYYKFDYSTYGGGDPATNSITMSAIKIAEQLGIDVIVATTYSGYTARALSRFRRN
IKIVAASPRITTYHRLALIWGVTPVIMQKFTDTDNMLESVSNIVKSLDFGVSGENIIVTAGIPYGFSSKTNLLKVHEI

Sequences:

>Translated_478_residues
MNEKIENKKTRIVCTIGPATQDETMIKKLINAGMNVARLNTSHDTIADHEKRVNLIKKIRKDLNIPFAILLDLEGPKIRT
GKFETDQVMLEEGQKFILTIEEIVGNKERVSINYRELPKEVKKGDFILLDDGKIRLVVVSSNEKEIVTKVVTGGSITHRR
GINVPGIDISLPPLTEKDMEYLNKAVEWNVDYIAQSFVRKAEDITRTRRILTELGMPDLPIIAKIETLQALDNLESIIEE
ADGVMVARGDLGVEAPVEQIPLLQKRIIEIANTMAKPAITATQMLESMVNNPFPTRAEATDIANAILDGTDAVMLSEETS
IGKYPEQAVKVMANVAKETEKILEEYYYKFDYSTYGGGDPATNSITMSAIKIAEQLGIDVIVATTYSGYTARALSRFRRN
IKIVAASPRITTYHRLALIWGVTPVIMQKFTDTDNMLESVSNIVKSLDFGVSGENIIVTAGIPYGFSSKTNLLKVHEI
>Mature_478_residues
MNEKIENKKTRIVCTIGPATQDETMIKKLINAGMNVARLNTSHDTIADHEKRVNLIKKIRKDLNIPFAILLDLEGPKIRT
GKFETDQVMLEEGQKFILTIEEIVGNKERVSINYRELPKEVKKGDFILLDDGKIRLVVVSSNEKEIVTKVVTGGSITHRR
GINVPGIDISLPPLTEKDMEYLNKAVEWNVDYIAQSFVRKAEDITRTRRILTELGMPDLPIIAKIETLQALDNLESIIEE
ADGVMVARGDLGVEAPVEQIPLLQKRIIEIANTMAKPAITATQMLESMVNNPFPTRAEATDIANAILDGTDAVMLSEETS
IGKYPEQAVKVMANVAKETEKILEEYYYKFDYSTYGGGDPATNSITMSAIKIAEQLGIDVIVATTYSGYTARALSRFRRN
IKIVAASPRITTYHRLALIWGVTPVIMQKFTDTDNMLESVSNIVKSLDFGVSGENIIVTAGIPYGFSSKTNLLKVHEI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=36.5461847389558, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=36.5461847389558, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI33286418, Length=498, Percent_Identity=36.7469879518072, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI32967597, Length=500, Percent_Identity=35.4, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI10835121, Length=500, Percent_Identity=35.4, Blast_Score=279, Evalue=4e-75,
Organism=Homo sapiens, GI310128732, Length=294, Percent_Identity=39.4557823129252, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128730, Length=294, Percent_Identity=39.4557823129252, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=37.603305785124, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=37.603305785124, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=37.1559633027523, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=39.5348837209302, Blast_Score=344, Evalue=8e-96,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=39.9159663865546, Blast_Score=311, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=38.6363636363636, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=37.2950819672131, Blast_Score=264, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=37.2950819672131, Blast_Score=264, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=37.2950819672131, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=37.4233128834356, Blast_Score=263, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=452, Percent_Identity=36.7256637168142, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=35.6997971602434, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24648964, Length=482, Percent_Identity=37.9668049792531, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI28571814, Length=482, Percent_Identity=37.9668049792531, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24648966, Length=417, Percent_Identity=37.6498800959233, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24581235, Length=434, Percent_Identity=33.4101382488479, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24646914, Length=183, Percent_Identity=44.8087431693989, Blast_Score=158, Evalue=7e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53155; Mature: 53155

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKIENKKTRIVCTIGPATQDETMIKKLINAGMNVARLNTSHDTIADHEKRVNLIKKIR
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
KDLNIPFAILLDLEGPKIRTGKFETDQVMLEEGQKFILTIEEIVGNKERVSINYRELPKE
HHCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCEEEEEHHHHHCCCEEEEECHHHHHHH
VKKGDFILLDDGKIRLVVVSSNEKEIVTKVVTGGSITHRRGINVPGIDISLPPLTEKDME
HCCCCEEEEECCCEEEEEECCCCCEEHHHHCCCCCEECCCCCCCCCCCEECCCCCHHHHH
YLNKAVEWNVDYIAQSFVRKAEDITRTRRILTELGMPDLPIIAKIETLQALDNLESIIEE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ADGVMVARGDLGVEAPVEQIPLLQKRIIEIANTMAKPAITATQMLESMVNNPFPTRAEAT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
DIANAILDGTDAVMLSEETSIGKYPEQAVKVMANVAKETEKILEEYYYKFDYSTYGGGDP
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ATNSITMSAIKIAEQLGIDVIVATTYSGYTARALSRFRRNIKIVAASPRITTYHRLALIW
CCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
GVTPVIMQKFTDTDNMLESVSNIVKSLDFGVSGENIIVTAGIPYGFSSKTNLLKVHEI
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MNEKIENKKTRIVCTIGPATQDETMIKKLINAGMNVARLNTSHDTIADHEKRVNLIKKIR
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
KDLNIPFAILLDLEGPKIRTGKFETDQVMLEEGQKFILTIEEIVGNKERVSINYRELPKE
HHCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCEEEEEHHHHHCCCEEEEECHHHHHHH
VKKGDFILLDDGKIRLVVVSSNEKEIVTKVVTGGSITHRRGINVPGIDISLPPLTEKDME
HCCCCEEEEECCCEEEEEECCCCCEEHHHHCCCCCEECCCCCCCCCCCEECCCCCHHHHH
YLNKAVEWNVDYIAQSFVRKAEDITRTRRILTELGMPDLPIIAKIETLQALDNLESIIEE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ADGVMVARGDLGVEAPVEQIPLLQKRIIEIANTMAKPAITATQMLESMVNNPFPTRAEAT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
DIANAILDGTDAVMLSEETSIGKYPEQAVKVMANVAKETEKILEEYYYKFDYSTYGGGDP
HHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ATNSITMSAIKIAEQLGIDVIVATTYSGYTARALSRFRRNIKIVAASPRITTYHRLALIW
CCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
GVTPVIMQKFTDTDNMLESVSNIVKSLDFGVSGENIIVTAGIPYGFSSKTNLLKVHEI
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]