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The map label for this gene is pfkA [H]

Identifier: 160903345

GI number: 160903345

Start: 2121021

End: 2122028

Strand: Direct

Name: pfkA [H]

Synonym: Pmob_1916

Alternate gene names: 160903345

Gene position: 2121021-2122028 (Clockwise)

Preceding gene: 160903343

Following gene: 160903346

Centisome position: 97.76

GC content: 38.39

Gene sequence:

>1008_bases
ATGAAGTATAAAATAATAAAGATTTTTAGGAGGGATACAGTGAGTGAGGTTAAAAAAATCGGTTTAATGACCAGTGGTGG
AGATGCACCTGGAATGAACGCTGTTATTAGGGCTGTTACAAGAAGTGCAGTAGTGGAAAATATCGAAGTGTATGGGTTTT
TGAGAGGATTTGCTGGTATTTTAGATAAAGAATATAAAAAACTAACATATTCAGATGTTGGTGGAATAATGGAAAGAGGG
GGAACCATTTTAAGAACCTCTCGAGTACCAGAATTCAAAGTTCCTGAAGTTAGAAAAAAAGCTGCTGATATATTGAAGGA
TTTAGGTATAGATGTGTTAGTTATAATTGGGGGTAACGGGAGTCTTACTGGGGGAAAGTTGCTAGCAGAAGAACAAGGGA
TATCTGTAGTAGGCGTACCGGCTTCTATAGACAACGATATTGCATATACCGATATGAGCATAGGTGTTGATACCTGTCTT
AACACAGTAGTTGATGCCATGCAAAAGTTAAAAGATACCGCTTCTTCCCACGAAAGGGCTTTTATCGTCGAAGTTATGGG
AAGGACATCTGGATACATAGCGTTGATGTCAGGTCTTGCTATAGGTGCGGAAGCTATAATAATACCAGAAGTTCCCACCG
ATTACGATGCTTTAGCAGAAAAAATGTGGGATGAAAGAAAAAGGGGTAAGATTAATTCAATAGTCGTTGTTGCGGAGGGA
TCTGCCAGCGCCTATACAGTCGCCAGACATTTAGAAAATAGAATTGGTTTTGAAACGCGTATAACAATTTTAGGCCATAT
TCAAAGAGGGGGTTCTCCTACTGCATTCGATAGAATTTTGGCTTCGAGAATGGGGAACGAAGTAGTAAAATCAATTAAAG
ACGGAAATTTTAACGTGATGGTGGGCTTGAGTAAAAACGATTTAATAAGAACACCTCTTGAAAAAGTGCTTTCTGAAAAT
AATAAATTGGATATGGAGATTGTAAAGCTGGCAGGGATTTTATCCTAG

Upstream 100 bases:

>100_bases
AAAGGTAGCTTAAAGAGCAGAGAGCAAAGAATTGCGACCTTATATCTTAATGGGCAAGGATTGGGGCGACGGGGCGCTAA
ATGAATTTTGGGATTTCTAA

Downstream 100 bases:

>100_bases
GGCGTTCAAGGGGTAAACCCCTTGAAAATTCCCAAGGCATGGAATCTAAAAAAAAGATGTAATTTGGGAAGAAACACCCA
AAAGTTACGCATCTTTAGGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKYKIIKIFRRDTVSEVKKIGLMTSGGDAPGMNAVIRAVTRSAVVENIEVYGFLRGFAGILDKEYKKLTYSDVGGIMERG
GTILRTSRVPEFKVPEVRKKAADILKDLGIDVLVIIGGNGSLTGGKLLAEEQGISVVGVPASIDNDIAYTDMSIGVDTCL
NTVVDAMQKLKDTASSHERAFIVEVMGRTSGYIALMSGLAIGAEAIIIPEVPTDYDALAEKMWDERKRGKINSIVVVAEG
SASAYTVARHLENRIGFETRITILGHIQRGGSPTAFDRILASRMGNEVVKSIKDGNFNVMVGLSKNDLIRTPLEKVLSEN
NKLDMEIVKLAGILS

Sequences:

>Translated_335_residues
MKYKIIKIFRRDTVSEVKKIGLMTSGGDAPGMNAVIRAVTRSAVVENIEVYGFLRGFAGILDKEYKKLTYSDVGGIMERG
GTILRTSRVPEFKVPEVRKKAADILKDLGIDVLVIIGGNGSLTGGKLLAEEQGISVVGVPASIDNDIAYTDMSIGVDTCL
NTVVDAMQKLKDTASSHERAFIVEVMGRTSGYIALMSGLAIGAEAIIIPEVPTDYDALAEKMWDERKRGKINSIVVVAEG
SASAYTVARHLENRIGFETRITILGHIQRGGSPTAFDRILASRMGNEVVKSIKDGNFNVMVGLSKNDLIRTPLEKVLSEN
NKLDMEIVKLAGILS
>Mature_335_residues
MKYKIIKIFRRDTVSEVKKIGLMTSGGDAPGMNAVIRAVTRSAVVENIEVYGFLRGFAGILDKEYKKLTYSDVGGIMERG
GTILRTSRVPEFKVPEVRKKAADILKDLGIDVLVIIGGNGSLTGGKLLAEEQGISVVGVPASIDNDIAYTDMSIGVDTCL
NTVVDAMQKLKDTASSHERAFIVEVMGRTSGYIALMSGLAIGAEAIIIPEVPTDYDALAEKMWDERKRGKINSIVVVAEG
SASAYTVARHLENRIGFETRITILGHIQRGGSPTAFDRILASRMGNEVVKSIKDGNFNVMVGLSKNDLIRTPLEKVLSEN
NKLDMEIVKLAGILS

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=334, Percent_Identity=38.3233532934132, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453619, Length=333, Percent_Identity=38.4384384384384, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI266453768, Length=333, Percent_Identity=38.4384384384384, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI266453748, Length=333, Percent_Identity=38.4384384384384, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4505749, Length=333, Percent_Identity=38.4384384384384, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI11321601, Length=333, Percent_Identity=38.4384384384384, Blast_Score=222, Evalue=3e-58,
Organism=Escherichia coli, GI1790350, Length=294, Percent_Identity=44.2176870748299, Blast_Score=241, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25147584, Length=337, Percent_Identity=36.4985163204748, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=35.7142857142857, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=361, Percent_Identity=29.0858725761773, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323861, Length=352, Percent_Identity=37.7840909090909, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321679, Length=333, Percent_Identity=39.039039039039, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652337, Length=334, Percent_Identity=39.8203592814371, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI17647809, Length=334, Percent_Identity=39.8203592814371, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI28573326, Length=334, Percent_Identity=38.3233532934132, Blast_Score=219, Evalue=1e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36249; Mature: 36249

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKIIKIFRRDTVSEVKKIGLMTSGGDAPGMNAVIRAVTRSAVVENIEVYGFLRGFAGI
CCEEEEHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKEYKKLTYSDVGGIMERGGTILRTSRVPEFKVPEVRKKAADILKDLGIDVLVIIGGNG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
SLTGGKLLAEEQGISVVGVPASIDNDIAYTDMSIGVDTCLNTVVDAMQKLKDTASSHERA
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
FIVEVMGRTSGYIALMSGLAIGAEAIIIPEVPTDYDALAEKMWDERKRGKINSIVVVAEG
EEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
SASAYTVARHLENRIGFETRITILGHIQRGGSPTAFDRILASRMGNEVVKSIKDGNFNVM
CCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGLSKNDLIRTPLEKVLSENNKLDMEIVKLAGILS
EECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKYKIIKIFRRDTVSEVKKIGLMTSGGDAPGMNAVIRAVTRSAVVENIEVYGFLRGFAGI
CCEEEEHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKEYKKLTYSDVGGIMERGGTILRTSRVPEFKVPEVRKKAADILKDLGIDVLVIIGGNG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
SLTGGKLLAEEQGISVVGVPASIDNDIAYTDMSIGVDTCLNTVVDAMQKLKDTASSHERA
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
FIVEVMGRTSGYIALMSGLAIGAEAIIIPEVPTDYDALAEKMWDERKRGKINSIVVVAEG
EEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
SASAYTVARHLENRIGFETRITILGHIQRGGSPTAFDRILASRMGNEVVKSIKDGNFNVM
CCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGLSKNDLIRTPLEKVLSENNKLDMEIVKLAGILS
EECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]