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The map label for this gene is leuC [H]

Identifier: 160903139

GI number: 160903139

Start: 1867787

End: 1869046

Strand: Direct

Name: leuC [H]

Synonym: Pmob_1704

Alternate gene names: 160903139

Gene position: 1867787-1869046 (Clockwise)

Preceding gene: 160903138

Following gene: 160903140

Centisome position: 86.09

GC content: 38.97

Gene sequence:

>1260_bases
ATGTCAACTTACAAAACAATATCTGAAAAGATATTCAGTGATCATTTGGGAAGAGATGTTGAAGCGGGGGAAATAGTCAT
AGTTGATGTTGATTTCATGATGGGGCAAGATGGAACCACTCCATTAGCTATTAAGACGTTTGAAAATGTTGGAGCTAAAA
AGGTTGCCTACCCTGAAAAAGTAGCCTTTGTAATAGACCACAACGCTCCCAGTCCAAGTGAAAAGGTTTCTGCGTTACAT
AAATTGATGAGAGATTTTAGTAGCAATTACGGTACCAAGTTTTACGATATCGGTGAGGGCATATGTCATCAACTAATGCC
CGAAAAAGGGCATGCCCTACCTGGACAGATCGTAATAGGGGCTGATTCTCACACGTGTACTTATGGGGCCCTTAATTGTT
TCTCAACGGGTGTCGGTTCAACAGATTTGGCTATTGCTATGGCGAGTGGTAAGTTATGGTTTAAGGTACCACAAAGTATA
AAAATTGTTTTAGAAGGTTCTCTTTCAAAAGGTATTTATTCTAAGGACGTTGCGTTGTATGTTATAAAGAATATGACCGC
CAATGGAGCAACTTACAAGGCTGTAGAGTTTGAGGGCCCAGTTATTGATAATTTATCGGTTGAGGCAAGATTTACCATTT
CTAATATGTCAGTGGAAATGGGAGCCAAAGTCGGTTTGATGAAACTTGATGAAAAAGCTGAAAATTGGATAAAAGAAAGA
ACTGATAAACCATTTAAAAGTTACGAATCAGACGAAGGTGCAAATTATGAAAAGATATACAAATTTGATGTCAGCGATTT
GGAACCTCAAGTTGCCAAACCCCATGCCGTTGATAATGTTAGTCCAATTAGTGAAGTTGAAGGTATTCCTATACATCAAG
GTTTGTTAGGTACGTGCACTAACGGAAGAATTGAAGATCTAAGAATAGCTGCGAATATCTTAAAAGGCAGAAAAATCCAT
AAGGGTGTTAGACTTATTGTGGCTCCTGCATCTAAAGATACTTTATTAAAAGCTATGAATGAGGGAATAATTCAAACTTT
AATTCAAGCTGGAGCTACGGTAGTTGCCCCAGGCTGTGGGCCTTGTGTGGGTACACATAATGGAGTACCTTCAGATGGCG
AGAATGTAATTTCTACAGCAAATAGAAACTTCAAAGGAAGAATGGGAAACAACAAAGCTTTCATCTATTTGGGATCACCT
GCCACAGTGGCAGCTTCTTGTATTGAGGGAAAAATAGCGGATCCCCGAAAATATTTGTGA

Upstream 100 bases:

>100_bases
TTAAAAAGGGTAAGATCCCTATCTGTTCAACTCAAAAGAAGCTTATTCGATAAAGAGTTAATCTATCTTTACAACGAAAT
GAGGGAGGAAAAGATAAAGG

Downstream 100 bases:

>100_bases
AAGTTAATTTTTAGAACGGGAGGATAAATAATGGAATTAAAAGGTTATTCTCATAAATTTGGTGATGATGTGAACACCGA
CTACATTATTTCTGGAAAAT

Product: homoaconitate hydratase family protein

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSTYKTISEKIFSDHLGRDVEAGEIVIVDVDFMMGQDGTTPLAIKTFENVGAKKVAYPEKVAFVIDHNAPSPSEKVSALH
KLMRDFSSNYGTKFYDIGEGICHQLMPEKGHALPGQIVIGADSHTCTYGALNCFSTGVGSTDLAIAMASGKLWFKVPQSI
KIVLEGSLSKGIYSKDVALYVIKNMTANGATYKAVEFEGPVIDNLSVEARFTISNMSVEMGAKVGLMKLDEKAENWIKER
TDKPFKSYESDEGANYEKIYKFDVSDLEPQVAKPHAVDNVSPISEVEGIPIHQGLLGTCTNGRIEDLRIAANILKGRKIH
KGVRLIVAPASKDTLLKAMNEGIIQTLIQAGATVVAPGCGPCVGTHNGVPSDGENVISTANRNFKGRMGNNKAFIYLGSP
ATVAASCIEGKIADPRKYL

Sequences:

>Translated_419_residues
MSTYKTISEKIFSDHLGRDVEAGEIVIVDVDFMMGQDGTTPLAIKTFENVGAKKVAYPEKVAFVIDHNAPSPSEKVSALH
KLMRDFSSNYGTKFYDIGEGICHQLMPEKGHALPGQIVIGADSHTCTYGALNCFSTGVGSTDLAIAMASGKLWFKVPQSI
KIVLEGSLSKGIYSKDVALYVIKNMTANGATYKAVEFEGPVIDNLSVEARFTISNMSVEMGAKVGLMKLDEKAENWIKER
TDKPFKSYESDEGANYEKIYKFDVSDLEPQVAKPHAVDNVSPISEVEGIPIHQGLLGTCTNGRIEDLRIAANILKGRKIH
KGVRLIVAPASKDTLLKAMNEGIIQTLIQAGATVVAPGCGPCVGTHNGVPSDGENVISTANRNFKGRMGNNKAFIYLGSP
ATVAASCIEGKIADPRKYL
>Mature_418_residues
STYKTISEKIFSDHLGRDVEAGEIVIVDVDFMMGQDGTTPLAIKTFENVGAKKVAYPEKVAFVIDHNAPSPSEKVSALHK
LMRDFSSNYGTKFYDIGEGICHQLMPEKGHALPGQIVIGADSHTCTYGALNCFSTGVGSTDLAIAMASGKLWFKVPQSIK
IVLEGSLSKGIYSKDVALYVIKNMTANGATYKAVEFEGPVIDNLSVEARFTISNMSVEMGAKVGLMKLDEKAENWIKERT
DKPFKSYESDEGANYEKIYKFDVSDLEPQVAKPHAVDNVSPISEVEGIPIHQGLLGTCTNGRIEDLRIAANILKGRKIHK
GVRLIVAPASKDTLLKAMNEGIIQTLIQAGATVVAPGCGPCVGTHNGVPSDGENVISTANRNFKGRMGNNKAFIYLGSPA
TVAASCIEGKIADPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=446, Percent_Identity=31.390134529148, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI8659555, Length=395, Percent_Identity=24.3037974683544, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=32.7586206896552, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87081781, Length=364, Percent_Identity=25.8241758241758, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI2367097, Length=397, Percent_Identity=27.2040302267003, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787531, Length=392, Percent_Identity=26.2755102040816, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=446, Percent_Identity=31.390134529148, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=31.3868613138686, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25149342, Length=299, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=411, Percent_Identity=23.3576642335766, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320440, Length=448, Percent_Identity=32.3660714285714, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=29.5983086680761, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=454, Percent_Identity=26.2114537444934, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI281365315, Length=446, Percent_Identity=30.9417040358744, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17864292, Length=446, Percent_Identity=30.9417040358744, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28571643, Length=445, Percent_Identity=32.3595505617978, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=31.3868613138686, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17137564, Length=448, Percent_Identity=25.2232142857143, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=26.7567567567568, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45151; Mature: 45020

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYKTISEKIFSDHLGRDVEAGEIVIVDVDFMMGQDGTTPLAIKTFENVGAKKVAYPEK
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHEECCCCCCCEEEEEHHCCCCCCCCCCCE
VAFVIDHNAPSPSEKVSALHKLMRDFSSNYGTKFYDIGEGICHQLMPEKGHALPGQIVIG
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEEE
ADSHTCTYGALNCFSTGVGSTDLAIAMASGKLWFKVPQSIKIVLEGSLSKGIYSKDVALY
CCCCEEEHHCHHHHHCCCCCCCEEEEEECCEEEEECCCEEEEEEECCCCCCCCCCCEEEE
VIKNMTANGATYKAVEFEGPVIDNLSVEARFTISNMSVEMGAKVGLMKLDEKAENWIKER
EEECCCCCCCEEEEEEECCCEECCCCEEEEEEEECCEEECCCCCCEEEHHHHHHHHHHHH
TDKPFKSYESDEGANYEKIYKFDVSDLEPQVAKPHAVDNVSPISEVEGIPIHQGLLGTCT
CCCCHHHCCCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECC
NGRIEDLRIAANILKGRKIHKGVRLIVAPASKDTLLKAMNEGIIQTLIQAGATVVAPGCG
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
PCVGTHNGVPSDGENVISTANRNFKGRMGNNKAFIYLGSPATVAASCIEGKIADPRKYL
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STYKTISEKIFSDHLGRDVEAGEIVIVDVDFMMGQDGTTPLAIKTFENVGAKKVAYPEK
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHEECCCCCCCEEEEEHHCCCCCCCCCCCE
VAFVIDHNAPSPSEKVSALHKLMRDFSSNYGTKFYDIGEGICHQLMPEKGHALPGQIVIG
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEEE
ADSHTCTYGALNCFSTGVGSTDLAIAMASGKLWFKVPQSIKIVLEGSLSKGIYSKDVALY
CCCCEEEHHCHHHHHCCCCCCCEEEEEECCEEEEECCCEEEEEEECCCCCCCCCCCEEEE
VIKNMTANGATYKAVEFEGPVIDNLSVEARFTISNMSVEMGAKVGLMKLDEKAENWIKER
EEECCCCCCCEEEEEEECCCEECCCCEEEEEEEECCEEECCCCCCEEEHHHHHHHHHHHH
TDKPFKSYESDEGANYEKIYKFDVSDLEPQVAKPHAVDNVSPISEVEGIPIHQGLLGTCT
CCCCHHHCCCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEECC
NGRIEDLRIAANILKGRKIHKGVRLIVAPASKDTLLKAMNEGIIQTLIQAGATVVAPGCG
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCC
PCVGTHNGVPSDGENVISTANRNFKGRMGNNKAFIYLGSPATVAASCIEGKIADPRKYL
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA