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The map label for this gene is leuD2 [H]

Identifier: 160903140

GI number: 160903140

Start: 1869077

End: 1869580

Strand: Direct

Name: leuD2 [H]

Synonym: Pmob_1705

Alternate gene names: 160903140

Gene position: 1869077-1869580 (Clockwise)

Preceding gene: 160903139

Following gene: 160903141

Centisome position: 86.15

GC content: 30.95

Gene sequence:

>504_bases
ATGGAATTAAAAGGTTATTCTCATAAATTTGGTGATGATGTGAACACCGACTACATTATTTCTGGAAAATACAAATTCAA
TACCATCGATATGGATGAATTATCTGTTCATTTGATGGAGGATCTAAGGCCAAATTTTTATAATGAAATCAAAAAAGGTG
ATTTCATTGTTGCAGGAGAAAATTTTGGTTGTGGTTCTTCTAGGGAACAAGCTCCTTTAGTGATAAAACATGCTGGTATA
AGTGCTGTTATAGCAACATCTTTCGCCAGAATATTTTATAGAAATTCCATAAATATTGGCTTACCTTTAGTTGAAGTTCC
AACTGATAATATAGAAGAAGGTGATTTATTGACTGTTGATCTAGAAAAAGGTGTAGTTAAGAATCTTACCAAAGATGAAA
TTTTGAAGATTAAATCTCTCCCCAAAGTGATGCTAAAGATTTTACAAAGTGGGGGTTTGGTTAATTATTACAAAAAATAT
GGTACTTTAGAATTAATGGAATAG

Upstream 100 bases:

>100_bases
GGGATCACCTGCCACAGTGGCAGCTTCTTGTATTGAGGGAAAAATAGCGGATCCCCGAAAATATTTGTGAAAGTTAATTT
TTAGAACGGGAGGATAAATA

Downstream 100 bases:

>100_bases
TAGGTGATTTTATGTATACAGTTACTTTGATTCCGGGTGATGGAATAGGTCCAGAAATAACCTCTGTAGTGGTAGAGATT
TTTGAACATTTGAAAGCTCC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MELKGYSHKFGDDVNTDYIISGKYKFNTIDMDELSVHLMEDLRPNFYNEIKKGDFIVAGENFGCGSSREQAPLVIKHAGI
SAVIATSFARIFYRNSINIGLPLVEVPTDNIEEGDLLTVDLEKGVVKNLTKDEILKIKSLPKVMLKILQSGGLVNYYKKY
GTLELME

Sequences:

>Translated_167_residues
MELKGYSHKFGDDVNTDYIISGKYKFNTIDMDELSVHLMEDLRPNFYNEIKKGDFIVAGENFGCGSSREQAPLVIKHAGI
SAVIATSFARIFYRNSINIGLPLVEVPTDNIEEGDLLTVDLEKGVVKNLTKDEILKIKSLPKVMLKILQSGGLVNYYKKY
GTLELME
>Mature_167_residues
MELKGYSHKFGDDVNTDYIISGKYKFNTIDMDELSVHLMEDLRPNFYNEIKKGDFIVAGENFGCGSSREQAPLVIKHAGI
SAVIATSFARIFYRNSINIGLPLVEVPTDNIEEGDLLTVDLEKGVVKNLTKDEILKIKSLPKVMLKILQSGGLVNYYKKY
GTLELME

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=165, Percent_Identity=32.7272727272727, Blast_Score=67, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=115, Percent_Identity=37.3913043478261, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18755; Mature: 18755

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKGYSHKFGDDVNTDYIISGKYKFNTIDMDELSVHLMEDLRPNFYNEIKKGDFIVAGE
CCCCCCCCCCCCCCCCCEEEECCEEECEECHHHHHHHHHHHCCCHHHHHHCCCCEEEECC
NFGCGSSREQAPLVIKHAGISAVIATSFARIFYRNSINIGLPLVEVPTDNIEEGDLLTVD
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEE
LEKGVVKNLTKDEILKIKSLPKVMLKILQSGGLVNYYKKYGTLELME
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCEEEECC
>Mature Secondary Structure
MELKGYSHKFGDDVNTDYIISGKYKFNTIDMDELSVHLMEDLRPNFYNEIKKGDFIVAGE
CCCCCCCCCCCCCCCCCEEEECCEEECEECHHHHHHHHHHHCCCHHHHHHCCCCEEEECC
NFGCGSSREQAPLVIKHAGISAVIATSFARIFYRNSINIGLPLVEVPTDNIEEGDLLTVD
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEE
LEKGVVKNLTKDEILKIKSLPKVMLKILQSGGLVNYYKKYGTLELME
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]