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The map label for this gene is aksA [H]

Identifier: 160903138

GI number: 160903138

Start: 1866667

End: 1867812

Strand: Direct

Name: aksA [H]

Synonym: Pmob_1703

Alternate gene names: 160903138

Gene position: 1866667-1867812 (Clockwise)

Preceding gene: 160903137

Following gene: 160903139

Centisome position: 86.04

GC content: 37.35

Gene sequence:

>1146_bases
GTGTATATAGTTGATACAACTTTAAGAGATGGCGAGCAAACTGCCGGGGTAGTATTTGCCAATGAAGAAAAGGTCCAAAT
AGCGAAGATGTTAGCAGAGTTAGGGGTTTATCAGATCGAAGCCGGGATTCCCACGATGGGTGGAGATGAAAAGGAAGCCA
TAAAAAAGATAAGTAAATTAGACTTAGGTGTATCAATTATGGGCTGGAATAGAGCAGTTATAAAGGATGTTGAAGAATCT
ATTGATTGTGGGGTTGATGCTGTTGCCATTTCTATTTCCACTTCAGATATTCACATCAAACATAAATTAAAGAAAGATAG
GGATTGGGTATTAAAAAGCATGGTAGATGCCACAAAATTTGCCAAAAAACACAATTTGTACGTGTCTGTAAACGCTGAAG
ATGCCTCTAGAACTGATATGGATTTTCTTATAAAGTTTGCCAAAGAGGCTAAAGAGGCAGGAGCGGATAGGTTAAGATAC
TGTGACACCGTTGGCATGATGGAACCATTTTCCATCTACGAACATATCAGAAGAATAATAGACGAAGTTGGTATAAATGT
TGAAATGCATACTCACAACGATTTTGGCTTAGCTACAGCTAATACATTGGCAGGAATAAGAGCGGGTGCTAAGTACGCAG
GTGTTACGGTAAATGGGTTAGGAGAAAGAGCAGGTAATGCAGCTTTAGAGGAAGTGGTGATGGCTCTTAAATACATTTAC
AAAAATGATTTGGGAATAAAAACTCACTTACTAAAAGAAGTTTGTGAATACGTTGCTAAGGCGTCAGGAAGAGTTCTTCC
TTTATCAAAAACAATCGTAGGCCATAATGTTTTTGCTCACGAGTCTGGCATTCATACAGACGGTGTATTAAAGGACTCCA
AAACATATGAATCCTTCACTCCTGAAGAAGTAGGATTACAAAGACAAATACTTATTGGAAAACATTCCGGGAGGAACGCA
ATTATTGCAAAATTCAAAGAATACGAAATAGATTTAACTCCTGAAGAAGGAGAAGAGATTTTAAAAAGGGTAAGATCCCT
ATCTGTTCAACTCAAAAGAAGCTTATTCGATAAAGAGTTAATCTATCTTTACAACGAAATGAGGGAGGAAAAGATAAAGG
ATGTCAACTTACAAAACAATATCTGA

Upstream 100 bases:

>100_bases
GTAAGTCTAGAGTTACAATAGAAACAAAAATTGAAAGGAGGGAGGGTACGATTCTTCTAAATCTAATGGAAAGAAGAACG
TACAAAAGAACGTGAGTAAC

Downstream 100 bases:

>100_bases
AAAGATATTCAGTGATCATTTGGGAAGAGATGTTGAAGCGGGGGAAATAGTCATAGTTGATGTTGATTTCATGATGGGGC
AAGATGGAACCACTCCATTA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MYIVDTTLRDGEQTAGVVFANEEKVQIAKMLAELGVYQIEAGIPTMGGDEKEAIKKISKLDLGVSIMGWNRAVIKDVEES
IDCGVDAVAISISTSDIHIKHKLKKDRDWVLKSMVDATKFAKKHNLYVSVNAEDASRTDMDFLIKFAKEAKEAGADRLRY
CDTVGMMEPFSIYEHIRRIIDEVGINVEMHTHNDFGLATANTLAGIRAGAKYAGVTVNGLGERAGNAALEEVVMALKYIY
KNDLGIKTHLLKEVCEYVAKASGRVLPLSKTIVGHNVFAHESGIHTDGVLKDSKTYESFTPEEVGLQRQILIGKHSGRNA
IIAKFKEYEIDLTPEEGEEILKRVRSLSVQLKRSLFDKELIYLYNEMREEKIKDVNLQNNI

Sequences:

>Translated_381_residues
MYIVDTTLRDGEQTAGVVFANEEKVQIAKMLAELGVYQIEAGIPTMGGDEKEAIKKISKLDLGVSIMGWNRAVIKDVEES
IDCGVDAVAISISTSDIHIKHKLKKDRDWVLKSMVDATKFAKKHNLYVSVNAEDASRTDMDFLIKFAKEAKEAGADRLRY
CDTVGMMEPFSIYEHIRRIIDEVGINVEMHTHNDFGLATANTLAGIRAGAKYAGVTVNGLGERAGNAALEEVVMALKYIY
KNDLGIKTHLLKEVCEYVAKASGRVLPLSKTIVGHNVFAHESGIHTDGVLKDSKTYESFTPEEVGLQRQILIGKHSGRNA
IIAKFKEYEIDLTPEEGEEILKRVRSLSVQLKRSLFDKELIYLYNEMREEKIKDVNLQNNI
>Mature_381_residues
MYIVDTTLRDGEQTAGVVFANEEKVQIAKMLAELGVYQIEAGIPTMGGDEKEAIKKISKLDLGVSIMGWNRAVIKDVEES
IDCGVDAVAISISTSDIHIKHKLKKDRDWVLKSMVDATKFAKKHNLYVSVNAEDASRTDMDFLIKFAKEAKEAGADRLRY
CDTVGMMEPFSIYEHIRRIIDEVGINVEMHTHNDFGLATANTLAGIRAGAKYAGVTVNGLGERAGNAALEEVVMALKYIY
KNDLGIKTHLLKEVCEYVAKASGRVLPLSKTIVGHNVFAHESGIHTDGVLKDSKTYESFTPEEVGLQRQILIGKHSGRNA
IIAKFKEYEIDLTPEEGEEILKRVRSLSVQLKRSLFDKELIYLYNEMREEKIKDVNLQNNI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=355, Percent_Identity=36.3380281690141, Blast_Score=191, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6320019, Length=351, Percent_Identity=31.3390313390313, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320071, Length=351, Percent_Identity=31.0541310541311, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24582381, Length=288, Percent_Identity=26.3888888888889, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42537; Mature: 42537

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIVDTTLRDGEQTAGVVFANEEKVQIAKMLAELGVYQIEAGIPTMGGDEKEAIKKISKL
CEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHH
DLGVSIMGWNRAVIKDVEESIDCGVDAVAISISTSDIHIKHKLKKDRDWVLKSMVDATKF
CCCEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHHHH
AKKHNLYVSVNAEDASRTDMDFLIKFAKEAKEAGADRLRYCDTVGMMEPFSIYEHIRRII
HHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
DEVGINVEMHTHNDFGLATANTLAGIRAGAKYAGVTVNGLGERAGNAALEEVVMALKYIY
HHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
KNDLGIKTHLLKEVCEYVAKASGRVLPLSKTIVGHNVFAHESGIHTDGVLKDSKTYESFT
HCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCC
PEEVGLQRQILIGKHSGRNAIIAKFKEYEIDLTPEEGEEILKRVRSLSVQLKRSLFDKEL
CHHHCCCEEEEEECCCCCCCEEEEHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYLYNEMREEKIKDVNLQNNI
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYIVDTTLRDGEQTAGVVFANEEKVQIAKMLAELGVYQIEAGIPTMGGDEKEAIKKISKL
CEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHH
DLGVSIMGWNRAVIKDVEESIDCGVDAVAISISTSDIHIKHKLKKDRDWVLKSMVDATKF
CCCEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHHHH
AKKHNLYVSVNAEDASRTDMDFLIKFAKEAKEAGADRLRYCDTVGMMEPFSIYEHIRRII
HHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
DEVGINVEMHTHNDFGLATANTLAGIRAGAKYAGVTVNGLGERAGNAALEEVVMALKYIY
HHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
KNDLGIKTHLLKEVCEYVAKASGRVLPLSKTIVGHNVFAHESGIHTDGVLKDSKTYESFT
HCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCC
PEEVGLQRQILIGKHSGRNAIIAKFKEYEIDLTPEEGEEILKRVRSLSVQLKRSLFDKEL
CHHHCCCEEEEEECCCCCCCEEEEHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYLYNEMREEKIKDVNLQNNI
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]