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The map label for this gene is truB [H]

Identifier: 160903127

GI number: 160903127

Start: 1848700

End: 1849617

Strand: Direct

Name: truB [H]

Synonym: Pmob_1692

Alternate gene names: 160903127

Gene position: 1848700-1849617 (Clockwise)

Preceding gene: 160903126

Following gene: 160903128

Centisome position: 85.21

GC content: 30.61

Gene sequence:

>918_bases
ATGAAAAGTGGTTTTTTAGTAATCAACAAGCCAAAGGGTATTACTTCTCATGATGTAATCAATATTCTTAGAAAAAAGTT
TGGCTTACAGAAAATTGGTCATGCTGGAACTCTAGATCCCTTTGCTACGGGAGTACTAGTTGTAGGAGTAAATAAAGCTA
CAAAATTGTTAGAATTTTTTCAAAACGAAAAAAAGACATACTATGTAAAAGCTGAATTGGGAATCATCACAGATACTTTT
GATATAGAAGGGAAAATACAGGAAAAGAACATAGTTACTCAGCAACACATCTCTAATTTGAAAGACATATGTTTTTCTTT
TGTGGGGGAATATCTACAAGTTCCTCCTGCCTATTCAGCCAAAAAATATAAAGGTAAAAAGCTTTATGAATATGCCCGTG
AAGGTAAAATAATTAATCTTCCTCCAAAAAAAGTAAAAATATATCAGCTTTCAAATTTTTCACAAGAAGGACAAGAATTT
AGTTTCTTTGTAGAAGTAAGTTCGGGAACTTATATAAGATCATTAATCATGGATATAGGATATGCTTTAGGATGTGGAGC
CGTTACCAAAGAACTTTGTAGAGTGAAAAGTGGTAAGTTTGAATTAAAAGATAGCATTTTGTTAGAAGAGGTTTCACTGG
AAAAAATGTTAAAAATGGATGAGGCTTTAGATCTTCCTTACGTTAAGATTAATAACGGGCAACTAGTCATTAAAGGGCAG
CAAATTTATAAAGATAATGTTTTAGAATTTTCTAATTTCAATAAAAATGATTACGTTAAAATATACGACGAAAAAGAATG
TTTTTTAGGAATAGGTAGGGCTGAAAAAAAATCGACATTTTTAAGAACCCTTCTTAAAGAAGAAGAAAGGAACGATAGGA
TAGTAAAAATATATAAAATTCTGTACGAGGTGACATAA

Upstream 100 bases:

>100_bases
TCAAGGTTCCCGAGGTAAGGTTTCATAAAGACGAAGGTATTGAAGCAAGTATTAGAATAAATAAACTAATAGAGAAAATA
GAGAAAAACGAGGAAGGGAA

Downstream 100 bases:

>100_bases
AATTGTATGCACTCACAATTGGGATCTTTGATGGTGTACACAAGGGCCATCAGTATATATTGAAAAACACTCTCCAACTC
GCGAATAACTTAAGGTTTGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKSGFLVINKPKGITSHDVINILRKKFGLQKIGHAGTLDPFATGVLVVGVNKATKLLEFFQNEKKTYYVKAELGIITDTF
DIEGKIQEKNIVTQQHISNLKDICFSFVGEYLQVPPAYSAKKYKGKKLYEYAREGKIINLPPKKVKIYQLSNFSQEGQEF
SFFVEVSSGTYIRSLIMDIGYALGCGAVTKELCRVKSGKFELKDSILLEEVSLEKMLKMDEALDLPYVKINNGQLVIKGQ
QIYKDNVLEFSNFNKNDYVKIYDEKECFLGIGRAEKKSTFLRTLLKEEERNDRIVKIYKILYEVT

Sequences:

>Translated_305_residues
MKSGFLVINKPKGITSHDVINILRKKFGLQKIGHAGTLDPFATGVLVVGVNKATKLLEFFQNEKKTYYVKAELGIITDTF
DIEGKIQEKNIVTQQHISNLKDICFSFVGEYLQVPPAYSAKKYKGKKLYEYAREGKIINLPPKKVKIYQLSNFSQEGQEF
SFFVEVSSGTYIRSLIMDIGYALGCGAVTKELCRVKSGKFELKDSILLEEVSLEKMLKMDEALDLPYVKINNGQLVIKGQ
QIYKDNVLEFSNFNKNDYVKIYDEKECFLGIGRAEKKSTFLRTLLKEEERNDRIVKIYKILYEVT
>Mature_305_residues
MKSGFLVINKPKGITSHDVINILRKKFGLQKIGHAGTLDPFATGVLVVGVNKATKLLEFFQNEKKTYYVKAELGIITDTF
DIEGKIQEKNIVTQQHISNLKDICFSFVGEYLQVPPAYSAKKYKGKKLYEYAREGKIINLPPKKVKIYQLSNFSQEGQEF
SFFVEVSSGTYIRSLIMDIGYALGCGAVTKELCRVKSGKFELKDSILLEEVSLEKMLKMDEALDLPYVKINNGQLVIKGQ
QIYKDNVLEFSNFNKNDYVKIYDEKECFLGIGRAEKKSTFLRTLLKEEERNDRIVKIYKILYEVT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=228, Percent_Identity=37.280701754386, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4503337, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI215599015, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI2367200, Length=228, Percent_Identity=36.4035087719298, Blast_Score=154, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17553978, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=44.0944881889764, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=232, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364187, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364185, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364183, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI62471759, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17975520, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34885; Mature: 34885

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSGFLVINKPKGITSHDVINILRKKFGLQKIGHAGTLDPFATGVLVVGVNKATKLLEFF
CCCCCEEEECCCCCCHHHHHHHHHHHHCHHHCCCCCCCCHHHCCEEEEECCHHHHHHHHH
QNEKKTYYVKAELGIITDTFDIEGKIQEKNIVTQQHISNLKDICFSFVGEYLQVPPAYSA
HCCCEEEEEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KKYKGKKLYEYAREGKIINLPPKKVKIYQLSNFSQEGQEFSFFVEVSSGTYIRSLIMDIG
CCCCCHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHH
YALGCGAVTKELCRVKSGKFELKDSILLEEVSLEKMLKMDEALDLPYVKINNGQLVIKGQ
HHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECH
QIYKDNVLEFSNFNKNDYVKIYDEKECFLGIGRAEKKSTFLRTLLKEEERNDRIVKIYKI
HHHHCCCEECCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LYEVT
HHHCC
>Mature Secondary Structure
MKSGFLVINKPKGITSHDVINILRKKFGLQKIGHAGTLDPFATGVLVVGVNKATKLLEFF
CCCCCEEEECCCCCCHHHHHHHHHHHHCHHHCCCCCCCCHHHCCEEEEECCHHHHHHHHH
QNEKKTYYVKAELGIITDTFDIEGKIQEKNIVTQQHISNLKDICFSFVGEYLQVPPAYSA
HCCCEEEEEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KKYKGKKLYEYAREGKIINLPPKKVKIYQLSNFSQEGQEFSFFVEVSSGTYIRSLIMDIG
CCCCCHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHH
YALGCGAVTKELCRVKSGKFELKDSILLEEVSLEKMLKMDEALDLPYVKINNGQLVIKGQ
HHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECH
QIYKDNVLEFSNFNKNDYVKIYDEKECFLGIGRAEKKSTFLRTLLKEEERNDRIVKIYKI
HHHHCCCEECCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LYEVT
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12499554 [H]