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The map label for this gene is argG [H]

Identifier: 160877312

GI number: 160877312

Start: 4975927

End: 4977165

Strand: Reverse

Name: argG [H]

Synonym: Sbal195_4210

Alternate gene names: 160877312

Gene position: 4977165-4975927 (Counterclockwise)

Preceding gene: 160877313

Following gene: 160877311

Centisome position: 93.08

GC content: 48.99

Gene sequence:

>1239_bases
ATGTCTATCGAGATAAAAAATACTGGCGTGAAAAAAGTGGTTTTAGCCTATTCAGGTGGTCTAGATACCTCGGCCATTAT
TCCTTGGTTAAAAGAAAACTACGATAACTGTGAAATCATCGCATTCTGCGCTGACGTTGGCCAAGGCGAAGAAGAGCTGG
TTGGCTTGACTGAAAAAGCCTTAGCTTCGGGGGCGTCTGAGTGCCATATCGTCGATCTGAAAGAAGAATTCGTTAAAGAA
TATATCTACCCAACGATTGCGAGCGGGGCGATTTACGAAGGCACTTATTTACTCGGTACGTCAATGGCGCGTCCTATCAT
TGCCAAAGCACAGGTTGAAGTGGCCCGTAAAGTGGGCGCCGATGCTCTGTGCCACGGTTGTACCGGTAAAGGTAACGATC
AAGTGCGTTTCGAAGGTTGTTTCGCTGCATTAGCGCCTGATTTAAAAGTGATTGCACCATGGCGTGAATGGACCATGCAG
AGCCGTGAAGATCTACTGGCCTATTTAGCCGAACGTGACATTAAGACGTCAGCGTCAGCGACTAAAATCTATAGCCGTGA
TGCCAATGCTTTCCATATTTCCCATGAAGGTGGTGAGTTAGAAGATCCATGGAACGAGCCAAGCAAAGGTGTGTGGACTC
TGACCGCCGCTCCTGAAGATGCACCGAACAAGCCTGAATATGTGTCGCTAGAAGTAGAACATGGCCGTATCACTAAAGTG
AATGGTGAAGCCCTAACGCCTTATGCTGCGCTGATGACATTAAACGCGATTGCTGCGCCACACGGTGTGGGTCGTATCGA
CATCACAGAAAACCGTTTAGTGGGCATGAAGTCTCGTGGCTGTTACGAAACACCCGGCGGCACCGTGATGTTCGCAGCGC
TGCGTGCGATTGAAGAACTTGTACTCGACAAAACCAGCCGTAACTGGCGTGAGCAAGTTGCCGCACAAATGGCGCATTTA
GTGTATGACGGCCGTTGGTTCACGCCACTGTGTAAGTCACTGCTGGCTGCTTCTGAGTCATTGGCTGAGTCAGTTAACGG
TGAAGTAGTGGTTAAACTCTACAAAGGCCAAGCGACGGCAGTGAAGAAACGTTCACCCAACAGCTTGTATTCTGAAGCGT
TTGCCACCTTCGGTGAAGATCAAGTGTACGACCAAAAACATGCAGAAGGCTTTATCCGCCTGTACTCATTAGCGAGCCGC
ATCCGCGCACTCAACGCTAATGACGTTAAGTCTAAGTAA

Upstream 100 bases:

>100_bases
GCATGCCCAAAATGCGGTACTGGTAACCTTATTTAGTTAAATTAACCCTGTTTAGCAGCCTGTTTTCTCCTAGGTGAGAA
ACGAGTCATAGGAATTTAAG

Downstream 100 bases:

>100_bases
TAGGTCGTTTAACAGGGCGCCTAGCGCCCTTTTTTGTTTCTAGGTTTTGATCAAGTTTTTTGATTCAGATGAGTCGATTT
CAAATCAGCAGTGAGTAAGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKE
YIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQ
SREDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTAAPEDAPNKPEYVSLEVEHGRITKV
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHL
VYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASR
IRALNANDVKSK

Sequences:

>Translated_412_residues
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKE
YIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQ
SREDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTAAPEDAPNKPEYVSLEVEHGRITKV
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHL
VYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASR
IRALNANDVKSK
>Mature_411_residues
SIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKEY
IYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQS
REDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTAAPEDAPNKPEYVSLEVEHGRITKVN
GEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHLV
YDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRI
RALNANDVKSK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=395, Percent_Identity=44.3037974683544, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI16950633, Length=395, Percent_Identity=44.3037974683544, Blast_Score=305, Evalue=4e-83,
Organism=Escherichia coli, GI1789563, Length=368, Percent_Identity=27.7173913043478, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=401, Percent_Identity=40.8977556109726, Blast_Score=291, Evalue=9e-80,
Organism=Drosophila melanogaster, GI21358151, Length=415, Percent_Identity=41.6867469879518, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45036; Mature: 44904

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKA
CEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKEYIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQSREDLLAYLAERDIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTAAPEDAPNKPEYVSLEVEHGRITKV
HHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEE
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEEL
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKTSRNWREQVAAQMAHLVYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATA
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
VKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRIRALNANDVKSK
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKA
EEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKEYIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQSREDLLAYLAERDIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTAAPEDAPNKPEYVSLEVEHGRITKV
HHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEE
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEEL
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKTSRNWREQVAAQMAHLVYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATA
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
VKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRIRALNANDVKSK
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA