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The map label for this gene is argF [H]

Identifier: 160877313

GI number: 160877313

Start: 4977226

End: 4978131

Strand: Reverse

Name: argF [H]

Synonym: Sbal195_4211

Alternate gene names: 160877313

Gene position: 4978131-4977226 (Counterclockwise)

Preceding gene: 160877314

Following gene: 160877312

Centisome position: 93.1

GC content: 47.02

Gene sequence:

>906_bases
ATGAAGCATTTACTATCGATAAAAGAGTTAACCCAGCAGCAGTTACTTGATCTGATTGCCTTGGCAAAAACGATCAAAGC
CAATCCGGCTGAGTACCGCCACGCGCTGGATGGCAAGAGTGTGGTGATGTTATTTGAAAAACCCTCGCTCAGAACACGCG
TTAGCTTTGATATTGGTATTAATAAACTTGGTGGCCACTGTTTATACCTAGACCAACAAAATGGTGCGCTAGGTCAACGG
GAATCGGTTGCTGACTTTGCTGCTAACCTGTCTTGCTGGGCCGATGCGATTGTGGCGAGAACCTATTCGCACACCACCAT
AGAACAATTAGCCGAGTGTGGCACAGTGCCCGTGATTAATGCCTTGTCGGATCTTTATCATCCTTGCCAAGCATTAGCCG
ACTTTTTAACACTGGCCGAGCAGTTTGAGGATGTCAGCAAAGCCAAATTAGCTTATGTTGGTGATGGTAATAATGTCACT
AACTCGCTGATGTACTGTGCGGCTATTCTCGGTGCTACCATGACAGTGATCTGCCCAGCGGGTCACTTTCCTGATGGCTA
CGTAGTGGCAGAAGTGCAAGAGCTTGCTAAGCGATATGGCGGTAAGCTGGTACTGACTTCAGATATCGATGCGATTGAAG
GTCATGATGCGATTTATACTGATACTTGGATTTCAATGGGCGATCCAACGCCGCTGGCGGATATCAAAGCGAAGTTTGCG
CCATACCAAGTAAACAAAGCCTTGATGATGAAAGCGGGCGCTAACTTCTTTATGCATTGTTTGCCCGCCCACCGTGGTGT
AGAAGTAACAGATGAAGTGATGGACGGTGCAGGCTCCTTAATTCTGCAACAAGCCGAAAATCGGATGCATGCCCAAAATG
CGGTACTGGTAACCTTATTTAGTTAA

Upstream 100 bases:

>100_bases
GGCGGTTCAAGTTGCCTCGTGGCGTGATGCAAGCCAATTAATCGCATTAGCAAAAGGTGAAGCCGTAGGAACACAAATCC
AACCATAAGTGGAGTAGGTT

Downstream 100 bases:

>100_bases
ATTAACCCTGTTTAGCAGCCTGTTTTCTCCTAGGTGAGAAACGAGTCATAGGAATTTAAGATGTCTATCGAGATAAAAAA
TACTGGCGTGAAAAAAGTGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKHLLSIKELTQQQLLDLIALAKTIKANPAEYRHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGQR
ESVADFAANLSCWADAIVARTYSHTTIEQLAECGTVPVINALSDLYHPCQALADFLTLAEQFEDVSKAKLAYVGDGNNVT
NSLMYCAAILGATMTVICPAGHFPDGYVVAEVQELAKRYGGKLVLTSDIDAIEGHDAIYTDTWISMGDPTPLADIKAKFA
PYQVNKALMMKAGANFFMHCLPAHRGVEVTDEVMDGAGSLILQQAENRMHAQNAVLVTLFS

Sequences:

>Translated_301_residues
MKHLLSIKELTQQQLLDLIALAKTIKANPAEYRHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGQR
ESVADFAANLSCWADAIVARTYSHTTIEQLAECGTVPVINALSDLYHPCQALADFLTLAEQFEDVSKAKLAYVGDGNNVT
NSLMYCAAILGATMTVICPAGHFPDGYVVAEVQELAKRYGGKLVLTSDIDAIEGHDAIYTDTWISMGDPTPLADIKAKFA
PYQVNKALMMKAGANFFMHCLPAHRGVEVTDEVMDGAGSLILQQAENRMHAQNAVLVTLFS
>Mature_301_residues
MKHLLSIKELTQQQLLDLIALAKTIKANPAEYRHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGQR
ESVADFAANLSCWADAIVARTYSHTTIEQLAECGTVPVINALSDLYHPCQALADFLTLAEQFEDVSKAKLAYVGDGNNVT
NSLMYCAAILGATMTVICPAGHFPDGYVVAEVQELAKRYGGKLVLTSDIDAIEGHDAIYTDTWISMGDPTPLADIKAKFA
PYQVNKALMMKAGANFFMHCLPAHRGVEVTDEVMDGAGSLILQQAENRMHAQNAVLVTLFS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=39.672131147541, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=27.6872964169381, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=37.0030581039755, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=36.5325077399381, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=29.8360655737705, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=33.2258064516129, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=158, Percent_Identity=29.746835443038, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 32720; Mature: 32720

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSIKELTQQQLLDLIALAKTIKANPAEYRHALDGKSVVMLFEKPSLRTRVSFDIGI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGQRESVADFAANLSCWADAIVARTYSHTTIEQLAECGTVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHH
ALSDLYHPCQALADFLTLAEQFEDVSKAKLAYVGDGNNVTNSLMYCAAILGATMTVICPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECC
GHFPDGYVVAEVQELAKRYGGKLVLTSDIDAIEGHDAIYTDTWISMGDPTPLADIKAKFA
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECEEECCCCCCHHHHHHHCC
PYQVNKALMMKAGANFFMHCLPAHRGVEVTDEVMDGAGSLILQQAENRMHAQNAVLVTLF
CHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEE
S
C
>Mature Secondary Structure
MKHLLSIKELTQQQLLDLIALAKTIKANPAEYRHALDGKSVVMLFEKPSLRTRVSFDIGI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGQRESVADFAANLSCWADAIVARTYSHTTIEQLAECGTVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHH
ALSDLYHPCQALADFLTLAEQFEDVSKAKLAYVGDGNNVTNSLMYCAAILGATMTVICPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECC
GHFPDGYVVAEVQELAKRYGGKLVLTSDIDAIEGHDAIYTDTWISMGDPTPLADIKAKFA
CCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECEEECCCCCCHHHHHHHCC
PYQVNKALMMKAGANFFMHCLPAHRGVEVTDEVMDGAGSLILQQAENRMHAQNAVLVTLF
CHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEE
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]