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The map label for this gene is dapF

Identifier: 160877193

GI number: 160877193

Start: 4821891

End: 4822718

Strand: Reverse

Name: dapF

Synonym: Sbal195_4089

Alternate gene names: 160877193

Gene position: 4822718-4821891 (Counterclockwise)

Preceding gene: 160877194

Following gene: 160877192

Centisome position: 90.19

GC content: 51.93

Gene sequence:

>828_bases
TTGATCCAATTCACTAAGATGCACGGACTGGGCAACGATTTTATGGTGGTCGATGGGGTCACGCAGAACGTGTTCTTTTC
GCCTGAGCAGATCCGCCGTTTAGCCGACCGTAACTTTGGTATAGGTTTCGACCAACTACTCTTAGTTGAGCCGCCTTATG
ATCCCGATTTAGATTTTCATTATCGCATCTTCAATGCCGATGGCACTGAAGTAGAACAATGCGGTAATGGCGCCCGCTGT
TTCGCCCGTTTTGTGCGTAATAAAGGCCTGACTAACAAGAGCAAAATCCGTGTCAGCACCTCATCGGGCAAGATGACTCT
GCGCTTAGAGCGAGACGGTACTGTTACCGTAAATATGGGCGTGCCGATCCTCGAACCGAGTCAGATCCCCTTCAAAGCCA
AGAAACCTGAAAAAACCTATTTACTGCAAACACCGATGCAAACCTTTTTGTGTGGCGCGGCTTCTATGGGTAATCCCCAT
TGCGTGCTCGACGTTGAAGATGTGGCGAGCGCTTCTGTCGCTGAAATTGGTGCCATGCTCACTAAGCATGAGCGTTTTCC
ACGCGGTGTGAATGTGGGATTTATGCAGGTCGTAGACTCCGGCCACATTAAGCTGCGGGTGTATGAGCGCGGCGCGGCCG
AGACCTTGGCCTGTGGCACAGGCGCATGTGCAGCTGTAGTTGTAGGTCAAGTGCAAGGAAAGCTCGGCCAGCAAGTGCGG
GTGGATTTACCCGGCGGCACCTTAACCATCAACTGGGAAGGCGAGGGTAAACCTTTGTGGATGACGGGCCCTGCGCAGCA
CGTTTACGATGGACAAATTCAGTTATGA

Upstream 100 bases:

>100_bases
GCAAGGTGAGCAACTCCTGCCGTAAACTAGAAAATTATATTGATTCAGGCGCTTGGTTTCATACTTTATGAGCGGCGCCT
CGGCTTAAAGGATAAGTATC

Downstream 100 bases:

>100_bases
CAGAGCCCTTGATGCCGCAGACGGATGTGCCTTTCGATGAGTTGATTATTCGCGAATATCTGCTGGATAATCCCGACTTT
TTTAATCGCTATCCCGAGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGTEVEQCGNGARC
FARFVRNKGLTNKSKIRVSTSSGKMTLRLERDGTVTVNMGVPILEPSQIPFKAKKPEKTYLLQTPMQTFLCGAASMGNPH
CVLDVEDVASASVAEIGAMLTKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL

Sequences:

>Translated_275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGTEVEQCGNGARC
FARFVRNKGLTNKSKIRVSTSSGKMTLRLERDGTVTVNMGVPILEPSQIPFKAKKPEKTYLLQTPMQTFLCGAASMGNPH
CVLDVEDVASASVAEIGAMLTKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
>Mature_275_residues
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGTEVEQCGNGARC
FARFVRNKGLTNKSKIRVSTSSGKMTLRLERDGTVTVNMGVPILEPSQIPFKAKKPEKTYLLQTPMQTFLCGAASMGNPH
CVLDVEDVASASVAEIGAMLTKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVR
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=69.3430656934307, Blast_Score=406, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SHEB2 (B8E690)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359794.1
- ProteinModelPortal:   B8E690
- SMR:   B8E690
- GeneID:   7086659
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3896
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30105; Mature: 30105

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGTEVEQCGNGARCFARFVRNKGLTNKSKIRVSTSSGKMTLRLERDGTVTVNMG
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECC
VPILEPSQIPFKAKKPEKTYLLQTPMQTFLCGAASMGNPHCVLDVEDVASASVAEIGAML
CCEECCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCEEEEEHHHHCCHHHHHHHHHH
TKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEE
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEC
>Mature Secondary Structure
MIQFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGTEVEQCGNGARCFARFVRNKGLTNKSKIRVSTSSGKMTLRLERDGTVTVNMG
EEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCEEEEECC
VPILEPSQIPFKAKKPEKTYLLQTPMQTFLCGAASMGNPHCVLDVEDVASASVAEIGAML
CCEECCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCEEEEEHHHHCCHHHHHHHHHH
TKHERFPRGVNVGFMQVVDSGHIKLRVYERGAAETLACGTGACAAVVVGQVQGKLGQQVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEE
VDLPGGTLTINWEGEGKPLWMTGPAQHVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA