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The map label for this gene is est [H]

Identifier: 160876884

GI number: 160876884

Start: 4455766

End: 4456683

Strand: Direct

Name: est [H]

Synonym: Sbal195_3780

Alternate gene names: 160876884

Gene position: 4455766-4456683 (Clockwise)

Preceding gene: 160876882

Following gene: 160876890

Centisome position: 83.33

GC content: 51.53

Gene sequence:

>918_bases
ATGAGCCAACTCTATTTAGAAGATGGAATTAAACAATTGGTTAGCGAGTTTATCGCGGCGGGTTGCCCATCGGTGCGAGA
GCAAACCATTGAGCAACGTAGGCAAGGTTATATCGCCAGCACGGTACTCGCAGGTGAAGCGGAAGACGTGTTCGAGGTGC
GCGCAGTTTCCATCGATGGCGCAGAATTGACCCTGTTCAAACCTTCGGCGGCAAACGATTTACCCGTTGTCATTTATTAC
CATGGCGGCTGTTTTGTCAGTGGCGGCGTTGAAACCCACAATCAACAACTTCGTAAACTTGCTAATGATGCTGGCGCACT
GGTGATTGCCGTTAGCTATCGTCTCGCCCCAGAATATGTTTATCCAGCCGCCCACGACGATGCCTGCCATGCCGCCGAGG
TCATTTATGCGCATTGCAAACAATGGGGTGGCGATAAGGACAAGATTACCCTGATGGGCGACAGCGCCGGCGGCCATTTA
GCCCTCGTCACTTGCCTACGTTTAAAAGCCAAAGGGCAATGGTTGCCACAAAAACAAGTACTTATCTATCCAATGCTCGA
TGCCACCGCTAAAAGCCAAAGCTATAGCGACAATGGCGAGAAATACATCATCACCCGCGACACCTTACTCACTGGATTTG
ATCTGTATTTAGATTGGCACCCCAGAACCGATGCCGAAGCCAGTCCGCTGCGAAGCACTGACTTAGCGGGACTGCCCGAA
ACCCATATCATCACAGCTGAATTTGATCCGCTCGTCGATGAAGGAGAACAGCTATTTAGGCATCTATTAGAGGCTGGCGT
GAATGCCCACTGCCGCCGCTATCTTGGGGTGATCCATGGCTTCTTCCAACTCTCGGGAGTGAGCCGTTCCGCTCGGGATG
CCATGGTGCAAGTGAGCAATATAGTGAAGACAGCCTAA

Upstream 100 bases:

>100_bases
TATTGCTTCTCATCACTGCGGCTACCATTTCAATTAAACTGCCGATAAACTCAGCCCATTGCAGAACTGCTAAGCACACA
GAAAAATGAGGCAAGATTAA

Downstream 100 bases:

>100_bases
CGTCATCCTTGATGATTAATTCCTTCATAAAAAAGGCTTTCTATCTAGATAGAAAGCCTTTTTAAACCGTTTGATATTCT
GACTTAAACGGCTTTCTTTC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSQLYLEDGIKQLVSEFIAAGCPSVREQTIEQRRQGYIASTVLAGEAEDVFEVRAVSIDGAELTLFKPSAANDLPVVIYY
HGGCFVSGGVETHNQQLRKLANDAGALVIAVSYRLAPEYVYPAAHDDACHAAEVIYAHCKQWGGDKDKITLMGDSAGGHL
ALVTCLRLKAKGQWLPQKQVLIYPMLDATAKSQSYSDNGEKYIITRDTLLTGFDLYLDWHPRTDAEASPLRSTDLAGLPE
THIITAEFDPLVDEGEQLFRHLLEAGVNAHCRRYLGVIHGFFQLSGVSRSARDAMVQVSNIVKTA

Sequences:

>Translated_305_residues
MSQLYLEDGIKQLVSEFIAAGCPSVREQTIEQRRQGYIASTVLAGEAEDVFEVRAVSIDGAELTLFKPSAANDLPVVIYY
HGGCFVSGGVETHNQQLRKLANDAGALVIAVSYRLAPEYVYPAAHDDACHAAEVIYAHCKQWGGDKDKITLMGDSAGGHL
ALVTCLRLKAKGQWLPQKQVLIYPMLDATAKSQSYSDNGEKYIITRDTLLTGFDLYLDWHPRTDAEASPLRSTDLAGLPE
THIITAEFDPLVDEGEQLFRHLLEAGVNAHCRRYLGVIHGFFQLSGVSRSARDAMVQVSNIVKTA
>Mature_304_residues
SQLYLEDGIKQLVSEFIAAGCPSVREQTIEQRRQGYIASTVLAGEAEDVFEVRAVSIDGAELTLFKPSAANDLPVVIYYH
GGCFVSGGVETHNQQLRKLANDAGALVIAVSYRLAPEYVYPAAHDDACHAAEVIYAHCKQWGGDKDKITLMGDSAGGHLA
LVTCLRLKAKGQWLPQKQVLIYPMLDATAKSQSYSDNGEKYIITRDTLLTGFDLYLDWHPRTDAEASPLRSTDLAGLPET
HIITAEFDPLVDEGEQLFRHLLEAGVNAHCRRYLGVIHGFFQLSGVSRSARDAMVQVSNIVKTA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=117, Percent_Identity=35.042735042735, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.8195488721804, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157041239, Length=156, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI61966717, Length=158, Percent_Identity=27.8481012658228, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1786682, Length=293, Percent_Identity=28.6689419795222, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=135, Percent_Identity=37.037037037037, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=124, Percent_Identity=37.9032258064516, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=152, Percent_Identity=30.9210526315789, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=113, Percent_Identity=36.283185840708, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33438; Mature: 33307

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLYLEDGIKQLVSEFIAAGCPSVREQTIEQRRQGYIASTVLAGEAEDVFEVRAVSIDG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEEEEEECC
AELTLFKPSAANDLPVVIYYHGGCFVSGGVETHNQQLRKLANDAGALVIAVSYRLAPEYV
CEEEEECCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCCEE
YPAAHDDACHAAEVIYAHCKQWGGDKDKITLMGDSAGGHLALVTCLRLKAKGQWLPQKQV
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
LIYPMLDATAKSQSYSDNGEKYIITRDTLLTGFDLYLDWHPRTDAEASPLRSTDLAGLPE
EEEEECCCHHCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
THIITAEFDPLVDEGEQLFRHLLEAGVNAHCRRYLGVIHGFFQLSGVSRSARDAMVQVSN
CEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IVKTA
HHHCC
>Mature Secondary Structure 
SQLYLEDGIKQLVSEFIAAGCPSVREQTIEQRRQGYIASTVLAGEAEDVFEVRAVSIDG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEEEEEECC
AELTLFKPSAANDLPVVIYYHGGCFVSGGVETHNQQLRKLANDAGALVIAVSYRLAPEYV
CEEEEECCCCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCCEE
YPAAHDDACHAAEVIYAHCKQWGGDKDKITLMGDSAGGHLALVTCLRLKAKGQWLPQKQV
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
LIYPMLDATAKSQSYSDNGEKYIITRDTLLTGFDLYLDWHPRTDAEASPLRSTDLAGLPE
EEEEECCCHHCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
THIITAEFDPLVDEGEQLFRHLLEAGVNAHCRRYLGVIHGFFQLSGVSRSARDAMVQVSN
CEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IVKTA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]