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The map label for this gene is rfbB [H]

Identifier: 160876139

GI number: 160876139

Start: 3598788

End: 3599867

Strand: Reverse

Name: rfbB [H]

Synonym: Sbal195_3030

Alternate gene names: 160876139

Gene position: 3599867-3598788 (Counterclockwise)

Preceding gene: 160876140

Following gene: 160876138

Centisome position: 67.32

GC content: 41.39

Gene sequence:

>1080_bases
ATGAAAGTTTTAGTAACAGGTGGTGCAGGTTTTATTGGTTCTGCTGTTGTGCGTCATATAATTTGTAATACTCAAGACAG
TGTGATTAATGTAGATAAACTGACCTATGCAGGTAATCTAGAGTCACTGACCAGTGTTGCTGACAATGCTCGCTATACAT
TTGAAAAAGTCGATATTTGTGACCGCGGTGAACTCGATAGGATTTTTTTACAGTATCAACCAGATGCTATTATGCATTTA
GCAGCAGAGTCCCATGTTGACCGCTCAATTACTGGCCCTTCAGATTTTATTCAGACCAATATTATTGGAACCTACACCTT
ATTAGAAGCTGCACGCCACTATTGGATACAACTCGATTCTGAGCGTAAAGCAGCATTTCGCTTTCATCATATTTCAACTG
ATGAAGTGTATGGCGATTTACCTCATCCAGATGAGCATGAAGGACAAGTGGTCAATCAAGAGCTGCCTTTATTTACTGAG
ACCACGCCTTATGCACCTAGCAGTCCATATTCTGCTTCTAAAGCTTCAAGCGACCATCTAGTACGCGCATGGTTGCGTAC
CTATGGCTTACCGACCATAGTGACCAACTGCTCAAATAACTACGGGCCTTATCATTTCCCTGAAAAGTTAATTCCACTGG
TGATATTGAATGCACTAGAAGGAAAGCCTTTACCTATTTACGGTAAAGGTGACCAAATTCGCGATTGGCTTTATGTTGAA
GATCATGCTCGCGCGCTATATAAAGTTGTTACGGAAGGTAAGGTTGGTGAAACCTACAATATTGGTGGGCATAACGAAAA
GAGAAATCTTGAAGTGGTGCAAACTATTTGCAGTATTTTAGATGTTCTTATGCCTAAAGGGACTCCTTACGCTGAGCAAA
TTACCTATGTAACGGATAGGCTAGGTCATGATCGTCGCTATGCTATCGATGCGAGCAAAATGAGTGCTGAACTAAATTGG
CAACCACAAGAAACCTTTGAAACGGGCCTCCTAAAAACTGTTGAATGGTATTTAGCAAACCAAGAATGGTGCCAGCATAT
TCAAGACGGCACTTATCAGCGAGAGCGTTTAGGCATTTAA

Upstream 100 bases:

>100_bases
TCGTGAAGTTATTGGCTTAAGTTTTATAAAGTCTGCTAAATGTTTTATCTTATACGCCTTTAAAGGCTTTATTAAAAGTC
GCATTTAAAAGAGAGTTAAA

Downstream 100 bases:

>100_bases
TCTCTTTTATTATTTTGTTTTAAAGCTCTGATATTTCTTTAAATCTAGGGCTAATTTATCTAGGAGCTTATTTTTTAATG
AAAGGAATTATTCTTGCGGG

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKVLVTGGAGFIGSAVVRHIICNTQDSVINVDKLTYAGNLESLTSVADNARYTFEKVDICDRGELDRIFLQYQPDAIMHL
AAESHVDRSITGPSDFIQTNIIGTYTLLEAARHYWIQLDSERKAAFRFHHISTDEVYGDLPHPDEHEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKRNLEVVQTICSILDVLMPKGTPYAEQITYVTDRLGHDRRYAIDASKMSAELNW
QPQETFETGLLKTVEWYLANQEWCQHIQDGTYQRERLGI

Sequences:

>Translated_359_residues
MKVLVTGGAGFIGSAVVRHIICNTQDSVINVDKLTYAGNLESLTSVADNARYTFEKVDICDRGELDRIFLQYQPDAIMHL
AAESHVDRSITGPSDFIQTNIIGTYTLLEAARHYWIQLDSERKAAFRFHHISTDEVYGDLPHPDEHEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKRNLEVVQTICSILDVLMPKGTPYAEQITYVTDRLGHDRRYAIDASKMSAELNW
QPQETFETGLLKTVEWYLANQEWCQHIQDGTYQRERLGI
>Mature_359_residues
MKVLVTGGAGFIGSAVVRHIICNTQDSVINVDKLTYAGNLESLTSVADNARYTFEKVDICDRGELDRIFLQYQPDAIMHL
AAESHVDRSITGPSDFIQTNIIGTYTLLEAARHYWIQLDSERKAAFRFHHISTDEVYGDLPHPDEHEGQVVNQELPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKRNLEVVQTICSILDVLMPKGTPYAEQITYVTDRLGHDRRYAIDASKMSAELNW
QPQETFETGLLKTVEWYLANQEWCQHIQDGTYQRERLGI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=33.3333333333333, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=24.4186046511628, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56237023, Length=304, Percent_Identity=28.6184210526316, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=304, Percent_Identity=28.6184210526316, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=304, Percent_Identity=28.6184210526316, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=77.3371104815864, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=70.1657458563536, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1786974, Length=361, Percent_Identity=25.207756232687, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788366, Length=200, Percent_Identity=30.5, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=345, Percent_Identity=36.231884057971, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=31.0650887573964, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=25.3644314868805, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=362, Percent_Identity=26.7955801104972, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=364, Percent_Identity=26.6483516483516, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=371, Percent_Identity=26.4150943396226, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=24.9266862170088, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.6304347826087, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40650; Mature: 40650

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGGAGFIGSAVVRHIICNTQDSVINVDKLTYAGNLESLTSVADNARYTFEKVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHEEECCCHHHHHHHHCCCCEEEEECCCC
DRGELDRIFLQYQPDAIMHLAAESHVDRSITGPSDFIQTNIIGTYTLLEAARHYWIQLDS
CCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
ERKAAFRFHHISTDEVYGDLPHPDEHEGQVVNQELPLFTETTPYAPSSPYSASKASSDHL
CCCEEEEEEECCHHHHCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVE
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEC
DHARALYKVVTEGKVGETYNIGGHNEKRNLEVVQTICSILDVLMPKGTPYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LGHDRRYAIDASKMSAELNWQPQETFETGLLKTVEWYLANQEWCQHIQDGTYQRERLGI
CCCCCEEEEEHHHHCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKVLVTGGAGFIGSAVVRHIICNTQDSVINVDKLTYAGNLESLTSVADNARYTFEKVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHEEECCCHHHHHHHHCCCCEEEEECCCC
DRGELDRIFLQYQPDAIMHLAAESHVDRSITGPSDFIQTNIIGTYTLLEAARHYWIQLDS
CCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
ERKAAFRFHHISTDEVYGDLPHPDEHEGQVVNQELPLFTETTPYAPSSPYSASKASSDHL
CCCEEEEEEECCHHHHCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVE
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEC
DHARALYKVVTEGKVGETYNIGGHNEKRNLEVVQTICSILDVLMPKGTPYAEQITYVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LGHDRRYAIDASKMSAELNWQPQETFETGLLKTVEWYLANQEWCQHIQDGTYQRERLGI
CCCCCEEEEEHHHHCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]