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The map label for this gene is murE [H]

Identifier: 160873533

GI number: 160873533

Start: 464082

End: 465581

Strand: Direct

Name: murE [H]

Synonym: Sbal195_0408

Alternate gene names: 160873533

Gene position: 464082-465581 (Clockwise)

Preceding gene: 160873532

Following gene: 160873534

Centisome position: 8.68

GC content: 51.8

Gene sequence:

>1500_bases
ATGATGTTATTACGGGATCTGCTCGCGCCTTGGCTGCATTATGCCGGCGCGCAGTCCTTCAGCGATTTGACCTTAGATAG
CCGCGCAATACGTCGCGGCGATGTGTTTTTGGCGCTGCCAGGACATAAGGTTGATGGCAGACAGTTTATTGAAAAAGCCT
TAGATTTAAGTGCCGCAGCCGTATTAGTCCACACGGATGATGCTGATCAGCATGGCAAAGTGTTGCACAGCGATAATGCC
GAGCACGGCGTACAGATTTATGTCTTCCAGTTGAGTCGCCAAGTATCAGCCATCGCCGCCCAGCGTTATCCCTTAGCGCA
TCAGTCTGCGACAACATCCCTTGGGGTGATTGGTATTACTGGCACCAATGGTAAAACCTCGACAAGCCAACTGATTGCCC
AGCTGATAACGTCGCTCGGCCGCAAAGCCGCCGTGATGGGCACCCTTGGCAATGGACTCTGGGGCGAGCTCGTCGATAGC
GGTAACACCACAGCCGATGCGATTACCCTAATGCGTCAGTTGCATGACTTTGAAGCCCAAGGCGCGAGTGTTTGCGCCAT
GGAAGTGTCGAGCCACGGCTTAGTGCAAGGCCGCGTCGATGCAGTACCATTCGATGTTGCCGTGTTTACCAATCTGACGC
GCGATCACCTCGATTACCACGGTGATATGGAAAGCTATGCCGCCGCTAAGCAAAGCCTATTTCGTTTCAGCACCTTAGGT
CACGGTTTATTGAATCTTGACGACGCGGTTGGTGCATCTTGGTTGACTGAACTTAAATCTGTGCCCGCGCAAATGTGGGG
CTTTAGTATTGAAGGTCATCAAGCCGCGGCTTTTTATACTAAAAATGCCAAGTTTGACGATCAAGGTGTCAGTGCAACCT
TAGTCTGGCCCGAGGGCGAGACTCAAATCCACTCACCACTTCTTGGTGCCTTTAATCTCTCTAATTTACTCGCGGCGCTC
TCAGCCTTGTATCTGCAAGGTTTTGATATGAAAGCGTTAGCCGAGCAAGTGCAATACCTAACGCCCGTCGCAGGTCGTAT
GGAGCGTTTCACAACTGCGAATAACATTACCTTAGTGGTCGATTATGCCCATACGCCAGATGCGATTGAACAAGCGTTAA
ACGCGCTACGTCGACATTGTGTCGGTGATTTATGGTGCGTATTTGGCTGTGGTGGCGACCGTGATAAGGGCAAGCGTCCT
TTGATGGGCCAAGCTGCAGAGCAATATGCTGACCGCATTATGGTGACGAGCGACAATGCGCGCAGTGAAGACCCCGCGCA
AATTATTACCGATATTATCCAAGGGCTAACCCACCCTCAGCAGGCATTGACTCAGGTTGACCGTATCGCAGCAATTAAAG
AGGTTGTGGCATTGGCGAAACCTGGCGATGTGATCTTGCTTGCGGGTAAAGGCCACGAAACTTACCAAGAGGCGGCAGGT
GTGCGTCATGACTATGATGAACGCGCCCTGGCACGCCAGTTATCGGAGCAATCAAGATGA

Upstream 100 bases:

>100_bases
TTGCCAAAGTCATGTCTGGCGCACTGCAAATGTTAAACGTTGAACCCATTACCGATAAAGAACAAGTGCAATTGGCGGGT
ATCGCCGCCGGGAGAGCTGA

Downstream 100 bases:

>100_bases
TCCCTCTTTCTCTCGAAACCCTAAGTCAACACTTAGGTACGTTTCGAGTCGGTGACGACGTCACCATCGAAGATTTAAGC
AGTGATAGCCGCAAAATCGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MMLLRDLLAPWLHYAGAQSFSDLTLDSRAIRRGDVFLALPGHKVDGRQFIEKALDLSAAAVLVHTDDADQHGKVLHSDNA
EHGVQIYVFQLSRQVSAIAAQRYPLAHQSATTSLGVIGITGTNGKTSTSQLIAQLITSLGRKAAVMGTLGNGLWGELVDS
GNTTADAITLMRQLHDFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYAAAKQSLFRFSTLG
HGLLNLDDAVGASWLTELKSVPAQMWGFSIEGHQAAAFYTKNAKFDDQGVSATLVWPEGETQIHSPLLGAFNLSNLLAAL
SALYLQGFDMKALAEQVQYLTPVAGRMERFTTANNITLVVDYAHTPDAIEQALNALRRHCVGDLWCVFGCGGDRDKGKRP
LMGQAAEQYADRIMVTSDNARSEDPAQIITDIIQGLTHPQQALTQVDRIAAIKEVVALAKPGDVILLAGKGHETYQEAAG
VRHDYDERALARQLSEQSR

Sequences:

>Translated_499_residues
MMLLRDLLAPWLHYAGAQSFSDLTLDSRAIRRGDVFLALPGHKVDGRQFIEKALDLSAAAVLVHTDDADQHGKVLHSDNA
EHGVQIYVFQLSRQVSAIAAQRYPLAHQSATTSLGVIGITGTNGKTSTSQLIAQLITSLGRKAAVMGTLGNGLWGELVDS
GNTTADAITLMRQLHDFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYAAAKQSLFRFSTLG
HGLLNLDDAVGASWLTELKSVPAQMWGFSIEGHQAAAFYTKNAKFDDQGVSATLVWPEGETQIHSPLLGAFNLSNLLAAL
SALYLQGFDMKALAEQVQYLTPVAGRMERFTTANNITLVVDYAHTPDAIEQALNALRRHCVGDLWCVFGCGGDRDKGKRP
LMGQAAEQYADRIMVTSDNARSEDPAQIITDIIQGLTHPQQALTQVDRIAAIKEVVALAKPGDVILLAGKGHETYQEAAG
VRHDYDERALARQLSEQSR
>Mature_499_residues
MMLLRDLLAPWLHYAGAQSFSDLTLDSRAIRRGDVFLALPGHKVDGRQFIEKALDLSAAAVLVHTDDADQHGKVLHSDNA
EHGVQIYVFQLSRQVSAIAAQRYPLAHQSATTSLGVIGITGTNGKTSTSQLIAQLITSLGRKAAVMGTLGNGLWGELVDS
GNTTADAITLMRQLHDFEAQGASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYAAAKQSLFRFSTLG
HGLLNLDDAVGASWLTELKSVPAQMWGFSIEGHQAAAFYTKNAKFDDQGVSATLVWPEGETQIHSPLLGAFNLSNLLAAL
SALYLQGFDMKALAEQVQYLTPVAGRMERFTTANNITLVVDYAHTPDAIEQALNALRRHCVGDLWCVFGCGGDRDKGKRP
LMGQAAEQYADRIMVTSDNARSEDPAQIITDIIQGLTHPQQALTQVDRIAAIKEVVALAKPGDVILLAGKGHETYQEAAG
VRHDYDERALARQLSEQSR

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=497, Percent_Identity=46.0764587525151, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 54040; Mature: 54040

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLLRDLLAPWLHYAGAQSFSDLTLDSRAIRRGDVFLALPGHKVDGRQFIEKALDLSAAA
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCEEE
VLVHTDDADQHGKVLHSDNAEHGVQIYVFQLSRQVSAIAAQRYPLAHQSATTSLGVIGIT
EEEECCCCCCCCCEEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
GTNGKTSTSQLIAQLITSLGRKAAVMGTLGNGLWGELVDSGNTTADAITLMRQLHDFEAQ
CCCCCCHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYAAAKQSLFRFSTLG
CCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
HGLLNLDDAVGASWLTELKSVPAQMWGFSIEGHQAAAFYTKNAKFDDQGVSATLVWPEGE
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCC
TQIHSPLLGAFNLSNLLAALSALYLQGFDMKALAEQVQYLTPVAGRMERFTTANNITLVV
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DYAHTPDAIEQALNALRRHCVGDLWCVFGCGGDRDKGKRPLMGQAAEQYADRIMVTSDNA
EECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEECCCC
RSEDPAQIITDIIQGLTHPQQALTQVDRIAAIKEVVALAKPGDVILLAGKGHETYQEAAG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCC
VRHDYDERALARQLSEQSR
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLLRDLLAPWLHYAGAQSFSDLTLDSRAIRRGDVFLALPGHKVDGRQFIEKALDLSAAA
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCEEE
VLVHTDDADQHGKVLHSDNAEHGVQIYVFQLSRQVSAIAAQRYPLAHQSATTSLGVIGIT
EEEECCCCCCCCCEEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
GTNGKTSTSQLIAQLITSLGRKAAVMGTLGNGLWGELVDSGNTTADAITLMRQLHDFEAQ
CCCCCCHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GASVCAMEVSSHGLVQGRVDAVPFDVAVFTNLTRDHLDYHGDMESYAAAKQSLFRFSTLG
CCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
HGLLNLDDAVGASWLTELKSVPAQMWGFSIEGHQAAAFYTKNAKFDDQGVSATLVWPEGE
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCC
TQIHSPLLGAFNLSNLLAALSALYLQGFDMKALAEQVQYLTPVAGRMERFTTANNITLVV
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DYAHTPDAIEQALNALRRHCVGDLWCVFGCGGDRDKGKRPLMGQAAEQYADRIMVTSDNA
EECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEECCCC
RSEDPAQIITDIIQGLTHPQQALTQVDRIAAIKEVVALAKPGDVILLAGKGHETYQEAAG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCC
VRHDYDERALARQLSEQSR
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]