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The map label for this gene is murF [H]

Identifier: 160873534

GI number: 160873534

Start: 465578

End: 466960

Strand: Direct

Name: murF [H]

Synonym: Sbal195_0409

Alternate gene names: 160873534

Gene position: 465578-466960 (Clockwise)

Preceding gene: 160873533

Following gene: 160873535

Centisome position: 8.71

GC content: 50.69

Gene sequence:

>1383_bases
ATGATCCCTCTTTCTCTCGAAACCCTAAGTCAACACTTAGGTACGTTTCGAGTCGGTGACGACGTCACCATCGAAGATTT
AAGCAGTGATAGCCGCAAAATCGGTGCTGCAACCCTGTTTGTTGCCTTAAAGGGTGAGCGTTTTGATGGCCATGATTTTG
CTGCAACAGCCATTGAGAACGGCGCCGCGGCGTTAATGGTCGAGCGCGAGTTAGCCTTTGATATTCCGCAGCTGATTGTG
CCCGATTGCCAAAAAGCCATGGGCGCGATAGGGGTTTATGTCCGTGACCAAATCAATCCCATTTGCGTGGCGTTAACTGG
CTCAAACGGCAAGACCAGCGTGAAGGAAATGATCGCGACCATTCTGTCGGCAAAGCATCAAATCCTCTATACCGCTGGTA
ACTTCAACAATGAAATCGGTGTGCCGCTTACCTTGCTGCGTTTAACGCCAGCGGATGAGTTTGGGATATTCGAACTCGGC
GCAAACCATAAAGGTGAAATTGATTACACCTCGGGACTCGTGCGTCCGAACGTGGCGCTGGTGAATAACGTCGGCAGTGC
CCACTTAGAAGGATTTGGATCGCAAGCGGGTGTCGCGCAGGCTAAGTCGGAAATTTTTAATCATCTGCAGGCCGATGGCA
CCGCCATCATCAACGCCGATGATGCCTTCGCCGATTTTATGAGCGTTAAGGCAAGACCTTATAAGCAACTGAGCTTCTCC
CAGCAAGATGGCGCCGCTAAACGTCATATCGACGTCATCGCTACAGGTCATAAGACCAATGCCGATGGTTGCTATCGTTT
TATGCTCAATTACTCAGGTGAATCTTGCCAAGTGGTACTACCGCTGGCGGGTCGCCATCAGGTGAGTAATGCACTAGCCG
CTGCAAGTGTTTGTATTGCCTTGGGATTAAGTTTGACAGAGATCGCCGAAGGCTTAAGCCAATTAACGCCAGTTAAAGGG
CGCATGCAGCCGAGTCAATTGGGACGCGTTCGTCTAATCGACGATAGCTATAACGCGAATCCTGTGTCCGTTGGGGCGGC
AATCGCTTGGTTAAAGGAAATTTCTGAAAATCGCTGTCTGGTACTGGGAGATTTAGGAGAATTAGGCGACAATGCGCCCC
TTTTACACGCTGAACTAGGGCAACTGGCCAAGCAACAGGGGATAGATGCCTTGTTTTGCACCGGAACGTTAAGTCAGCAC
ACGAGCCAAGCTTTCGGTGCGGAGCACTATGACAGTGTGGCGACGTTAGTAGAAAAACTCATAAAGCATATTAACCAGTT
ACCGGGACAGGTAACGGTTCTCGTTAAAGGTTCACGTAGCGCCGCCATGGAGCGGGTCGTGGATGGACTAACAGTAGCCT
TCGGGCGTGGGGAGTTAGTGTAA

Upstream 100 bases:

>100_bases
TGCTTGCGGGTAAAGGCCACGAAACTTACCAAGAGGCGGCAGGTGTGCGTCATGACTATGATGAACGCGCCCTGGCACGC
CAGTTATCGGAGCAATCAAG

Downstream 100 bases:

>100_bases
ATGCTGGTATATCTGGCCGAGTATTTAACCCGTTTTCATACCGGGTTTAACGTGTTTTCCTATGTGACGTTTCGAGCCAT
TTTAGGCTTATTAACCGCAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MIPLSLETLSQHLGTFRVGDDVTIEDLSSDSRKIGAATLFVALKGERFDGHDFAATAIENGAAALMVERELAFDIPQLIV
PDCQKAMGAIGVYVRDQINPICVALTGSNGKTSVKEMIATILSAKHQILYTAGNFNNEIGVPLTLLRLTPADEFGIFELG
ANHKGEIDYTSGLVRPNVALVNNVGSAHLEGFGSQAGVAQAKSEIFNHLQADGTAIINADDAFADFMSVKARPYKQLSFS
QQDGAAKRHIDVIATGHKTNADGCYRFMLNYSGESCQVVLPLAGRHQVSNALAAASVCIALGLSLTEIAEGLSQLTPVKG
RMQPSQLGRVRLIDDSYNANPVSVGAAIAWLKEISENRCLVLGDLGELGDNAPLLHAELGQLAKQQGIDALFCTGTLSQH
TSQAFGAEHYDSVATLVEKLIKHINQLPGQVTVLVKGSRSAAMERVVDGLTVAFGRGELV

Sequences:

>Translated_460_residues
MIPLSLETLSQHLGTFRVGDDVTIEDLSSDSRKIGAATLFVALKGERFDGHDFAATAIENGAAALMVERELAFDIPQLIV
PDCQKAMGAIGVYVRDQINPICVALTGSNGKTSVKEMIATILSAKHQILYTAGNFNNEIGVPLTLLRLTPADEFGIFELG
ANHKGEIDYTSGLVRPNVALVNNVGSAHLEGFGSQAGVAQAKSEIFNHLQADGTAIINADDAFADFMSVKARPYKQLSFS
QQDGAAKRHIDVIATGHKTNADGCYRFMLNYSGESCQVVLPLAGRHQVSNALAAASVCIALGLSLTEIAEGLSQLTPVKG
RMQPSQLGRVRLIDDSYNANPVSVGAAIAWLKEISENRCLVLGDLGELGDNAPLLHAELGQLAKQQGIDALFCTGTLSQH
TSQAFGAEHYDSVATLVEKLIKHINQLPGQVTVLVKGSRSAAMERVVDGLTVAFGRGELV
>Mature_460_residues
MIPLSLETLSQHLGTFRVGDDVTIEDLSSDSRKIGAATLFVALKGERFDGHDFAATAIENGAAALMVERELAFDIPQLIV
PDCQKAMGAIGVYVRDQINPICVALTGSNGKTSVKEMIATILSAKHQILYTAGNFNNEIGVPLTLLRLTPADEFGIFELG
ANHKGEIDYTSGLVRPNVALVNNVGSAHLEGFGSQAGVAQAKSEIFNHLQADGTAIINADDAFADFMSVKARPYKQLSFS
QQDGAAKRHIDVIATGHKTNADGCYRFMLNYSGESCQVVLPLAGRHQVSNALAAASVCIALGLSLTEIAEGLSQLTPVKG
RMQPSQLGRVRLIDDSYNANPVSVGAAIAWLKEISENRCLVLGDLGELGDNAPLLHAELGQLAKQQGIDALFCTGTLSQH
TSQAFGAEHYDSVATLVEKLIKHINQLPGQVTVLVKGSRSAAMERVVDGLTVAFGRGELV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=47.1238938053097, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48888; Mature: 48888

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLSLETLSQHLGTFRVGDDVTIEDLSSDSRKIGAATLFVALKGERFDGHDFAATAIEN
CCCCCHHHHHHHHCEEECCCCCCHHHCCCCCHHHCEEEEEEEEECCCCCCCCHHHHHHCC
GAAALMVERELAFDIPQLIVPDCQKAMGAIGVYVRDQINPICVALTGSNGKTSVKEMIAT
CCEEEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCHHHHHHHHH
ILSAKHQILYTAGNFNNEIGVPLTLLRLTPADEFGIFELGANHKGEIDYTSGLVRPNVAL
HHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCEECCCCCCCCCHHE
VNNVGSAHLEGFGSQAGVAQAKSEIFNHLQADGTAIINADDAFADFMSVKARPYKQLSFS
EECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCHHHCCCC
QQDGAAKRHIDVIATGHKTNADGCYRFMLNYSGESCQVVLPLAGRHQVSNALAAASVCIA
CCCCCCHHEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LGLSLTEIAEGLSQLTPVKGRMQPSQLGRVRLIDDSYNANPVSVGAAIAWLKEISENRCL
HCCCHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
VLGDLGELGDNAPLLHAELGQLAKQQGIDALFCTGTLSQHTSQAFGAEHYDSVATLVEKL
EEECCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHH
IKHINQLPGQVTVLVKGSRSAAMERVVDGLTVAFGRGELV
HHHHHHCCCEEEEEEECCHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MIPLSLETLSQHLGTFRVGDDVTIEDLSSDSRKIGAATLFVALKGERFDGHDFAATAIEN
CCCCCHHHHHHHHCEEECCCCCCHHHCCCCCHHHCEEEEEEEEECCCCCCCCHHHHHHCC
GAAALMVERELAFDIPQLIVPDCQKAMGAIGVYVRDQINPICVALTGSNGKTSVKEMIAT
CCEEEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCHHHHHHHHH
ILSAKHQILYTAGNFNNEIGVPLTLLRLTPADEFGIFELGANHKGEIDYTSGLVRPNVAL
HHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCEECCCCCCCCCHHE
VNNVGSAHLEGFGSQAGVAQAKSEIFNHLQADGTAIINADDAFADFMSVKARPYKQLSFS
EECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCHHHCCCC
QQDGAAKRHIDVIATGHKTNADGCYRFMLNYSGESCQVVLPLAGRHQVSNALAAASVCIA
CCCCCCHHEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LGLSLTEIAEGLSQLTPVKGRMQPSQLGRVRLIDDSYNANPVSVGAAIAWLKEISENRCL
HCCCHHHHHHHHHHCCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
VLGDLGELGDNAPLLHAELGQLAKQQGIDALFCTGTLSQHTSQAFGAEHYDSVATLVEKL
EEECCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHH
IKHINQLPGQVTVLVKGSRSAAMERVVDGLTVAFGRGELV
HHHHHHCCCEEEEEEECCHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]