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The map label for this gene is glnG [H]

Identifier: 160873392

GI number: 160873392

Start: 304810

End: 306222

Strand: Reverse

Name: glnG [H]

Synonym: Sbal195_0266

Alternate gene names: 160873392

Gene position: 306222-304810 (Counterclockwise)

Preceding gene: 160873393

Following gene: 160873387

Centisome position: 5.73

GC content: 51.88

Gene sequence:

>1413_bases
ATGCGAATTAGTGAACAAGTGTGGATCCTCGACGATGACAGCTCGATACGTTGGGTACTCGAAAAGGCACTTCAAGGTGC
CAAGCTCAGTACCGCCAGCTTTGCCGCCGCCGAATCCCTGTGGCAGGCCTTAGAAATATCGCAACCGCACGTGATAGTGT
CGGACATCCGTATGCCAGGCACAGACGGGTTAAGTCTGCTCGAACGATTGCAAGTACATTACCCCCATATTCCCGTGATC
ATTATGACGGCGCACTCGGACTTAGACAGCGCAGTCAGTGCCTATCAAGCGGGCGCATTTGAGTATCTGCCTAAACCCTT
CGACATCGATGAGGCGATCTCTCTGGTCGAGCGCGCACTCACCCATGCCACTGAACAGAGCCCAGCACCCGCGCAGGAAG
CCCAAGTCAAAACACCCGAAATCATAGGTGAAGCGCCAGCGATGCAGGAAGTATTTCGCGCCATAGGCAGGCTTTCACGA
TCATCCATCAGCGTGCTGATTAATGGCCAATCTGGGACAGGTAAAGAGCTCGTGGCAGGCGCGTTACACAAACACAGCCC
ACGTAAAGATAAGCCATTTATCGCGCTGAACATGGCTGCGATCCCTAAGGATTTGATCGAATCTGAGCTTTTCGGCCACG
AAAAAGGTGCCTTCACTGGCGCAGCCAATGTCCGCCAAGGTCGCTTCGAACAAGCGAACGGCGGCACGCTCTTTTTAGAT
GAAATCGGCGACATGCCATTAGACGTACAAACACGCTTACTCAGAGTACTTGCCGATGGCCAGTTCTACCGCGTCGGTGG
TCATAATGCGGTGCAAGTGGATGTGCGTATTATAGCGGCGACTCACCAAGATCTTGAATTATTAGTGCAAAAAGGCGGCT
TTAGGGAAGACTTATTCCATCGCCTCAACGTGATCCGCGTGCACTTACCGCCACTGTCACAAAGACGGGAAGACATTCCC
CAACTCGCCACCCATTTCCTCGCCTCGGCAGCGAAGGAAATTGGCGTGGAAACTAAGATCATGACCAAGGAAACCGCGGT
AAAACTCTCCCAATTACCTTGGCCCGGTAATGTCAGACAACTCGAAAACACCTGCCGCTGGTTAACCGTTATGGCATCGG
GGCAAGAAATTTTACCCCAAGATTTACCGCCAGAATTACTGAAAGATCCTGTGAGTGTCACCCACACGGCGAAAGGCAGC
CAAGATTGGCAATCGGCACTGACTGAGTGGATAGATCAAAAACTCTCGGAAGGAAACAGCGATCTATTAACTGAGGTACA
ACCCGCTTTCGAACGCATACTACTAGAAACTGCGCTGCGCCATACTCAAGGCCATAAACAGGAAGCGGCGAAACGTTTGG
GCTGGGGACGAAATACCTTAACGCGAAAACTCAAAGAGCTGTCGATGGATTGA

Upstream 100 bases:

>100_bases
GCCTCTCCAGCGCTGGGCACACCGAATTCATCATTTCATTACCGATTTTAGGCGCTAAATAATTTCGACAGTACCTACCC
ATACCGTATGAGGAAGCAAG

Downstream 100 bases:

>100_bases
GTAAATAAAATAACGTTATTACTCGCTATAGCGATACAAGCTTATTAATCATCAAAAAGTAAAAGGGATATATTCATATC
CCTTTTTATTTGAAATTCAT

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRISEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPHVIVSDIRMPGTDGLSLLERLQVHYPHIPVI
IMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAQEAQVKTPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHNAVQVDVRIIAATHQDLELLVQKGGFREDLFHRLNVIRVHLPPLSQRREDIP
QLATHFLASAAKEIGVETKIMTKETAVKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELLKDPVSVTHTAKGS
QDWQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD

Sequences:

>Translated_470_residues
MRISEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPHVIVSDIRMPGTDGLSLLERLQVHYPHIPVI
IMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAQEAQVKTPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHNAVQVDVRIIAATHQDLELLVQKGGFREDLFHRLNVIRVHLPPLSQRREDIP
QLATHFLASAAKEIGVETKIMTKETAVKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELLKDPVSVTHTAKGS
QDWQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
>Mature_470_residues
MRISEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPHVIVSDIRMPGTDGLSLLERLQVHYPHIPVI
IMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAQEAQVKTPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGHNAVQVDVRIIAATHQDLELLVQKGGFREDLFHRLNVIRVHLPPLSQRREDIP
QLATHFLASAAKEIGVETKIMTKETAVKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPPELLKDPVSVTHTAKGS
QDWQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=70.0431034482759, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=40.5117270788913, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790437, Length=461, Percent_Identity=41.8655097613883, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=39.2776523702032, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1789087, Length=337, Percent_Identity=40.3560830860534, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=42.814371257485, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082117, Length=336, Percent_Identity=41.3690476190476, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1787583, Length=330, Percent_Identity=41.2121212121212, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1786524, Length=372, Percent_Identity=40.0537634408602, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082152, Length=300, Percent_Identity=41, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87081872, Length=336, Percent_Identity=38.6904761904762, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=31.9620253164557, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=37.3333333333333, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789809, Length=137, Percent_Identity=31.3868613138686, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52037; Mature: 52037

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPHVIVSDIRMPG
CCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
TDGLSLLERLQVHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERAL
CCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
THATEQSPAPAQEAQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAG
HHHHCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
ALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
EIGDMPLDVQTRLLRVLADGQFYRVGGHNAVQVDVRIIAATHQDLELLVQKGGFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECHHHHHHHHHCCCCHHHHHH
RLNVIRVHLPPLSQRREDIPQLATHFLASAAKEIGVETKIMTKETAVKLSQLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCCHHH
LENTCRWLTVMASGQEILPQDLPPELLKDPVSVTHTAKGSQDWQSALTEWIDQKLSEGNS
HHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCH
DLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRISEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPHVIVSDIRMPG
CCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
TDGLSLLERLQVHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERAL
CCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
THATEQSPAPAQEAQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAG
HHHHCCCCCCCHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
ALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEH
EIGDMPLDVQTRLLRVLADGQFYRVGGHNAVQVDVRIIAATHQDLELLVQKGGFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECHHHHHHHHHCCCCHHHHHH
RLNVIRVHLPPLSQRREDIPQLATHFLASAAKEIGVETKIMTKETAVKLSQLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCCHHH
LENTCRWLTVMASGQEILPQDLPPELLKDPVSVTHTAKGSQDWQSALTEWIDQKLSEGNS
HHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCH
DLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]