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The map label for this gene is glnL [H]

Identifier: 160873393

GI number: 160873393

Start: 306261

End: 307307

Strand: Reverse

Name: glnL [H]

Synonym: Sbal195_0267

Alternate gene names: 160873393

Gene position: 307307-306261 (Counterclockwise)

Preceding gene: 160873394

Following gene: 160873392

Centisome position: 5.75

GC content: 49.76

Gene sequence:

>1047_bases
ATGGATAAAGAGACACTGCTCAATCATTTAGTTACCGCTGTACTTGTCATCGATAAGGATCTTAAGCCTTGTTATGCCAA
CGCAGCAGCAGAGCAATTATTAGGTGTTGGCAGCCATAGACTCGTAGAACAAGCTTTGCCTGAGCATTATCAAATCCTCG
GTGTCGAAGCCCAGTTATTGAGGGATGCGGTAACAGCTGGCCAAGGTTTAACGGTCAATACGGCGGCCCTAGTCACGCTC
GATGGTCAGCACCATACCGTTGATTTAACCCTTATTCCCCTCGATGACGAGGCTGAACTCAGCCTGCTCGAACTGCGCCA
AGTCGATCAACAAAGACGCATTCATCAGCAGCTGAGTCAAGATGCACAGCAACAAGCGGCGCAGTTTTTGGTGCGCAACT
TAGCCCATGAAATCAAAAATCCTTTAGGTGGTCTTCGCGGTGCGGCGCAATTGTTATCGCGGGAGCTGGATGATCCGGCG
CTGAAGGAATTCACTACCCTCATCATAGAGCAAGCCGATCGGCTGCGTAATTTAGTGGATCGCCTGCTCGGCCCCCAAAG
GCCAACACAGCACAGTCTGCACAACATCCATCAAGTGGTGCAAAAGGTCTATAAATTAGTTGAAATGGCGCTGCCCGCCA
ACATCCAACTCAAACGGGATTACGATCCATCCATTCCCGATATCGAGATGGACCCAGACCAAATGCAGCAAGCTGTGCTC
AACATTTTACAAAATGCGGTGCAGGCGCTTGAACATACGGGCGGCGAGATTTTGCTCCGCACTCGCACTCAACACCAAGT
CACCATAGGTTCACAGCGCCACAAGCTAGTGTTAACCCTATCGATTATCGACAACGGCCCCGGTATACCGCCTGAGCTGA
TGGACACATTATTTTATCCAATGGTGACAGGTCGTGAGCAAGGTTCAGGTTTAGGCCTCTCAATTGCCCATAATATTGCC
CGTTTACACTCGGGAAGAATCGATTGCCTCTCCAGCGCTGGGCACACCGAATTCATCATTTCATTACCGATTTTAGGCGC
TAAATAA

Upstream 100 bases:

>100_bases
AGATTAACCGCCCCAGACTAAACCGTTTGATGAAGACAAGTTGCAGCTTGCACCAGATTGGTGCACCATGGTGGTGCAAC
CCTATTTCTAGGAACGGTCA

Downstream 100 bases:

>100_bases
TTTCGACAGTACCTACCCATACCGTATGAGGAAGCAAGATGCGAATTAGTGAACAAGTGTGGATCCTCGACGATGACAGC
TCGATACGTTGGGTACTCGA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQILGVEAQLLRDAVTAGQGLTVNTAALVTL
DGQHHTVDLTLIPLDDEAELSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
LKEFTTLIIEQADRLRNLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILLRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHSGRIDCLSSAGHTEFIISLPILGAK

Sequences:

>Translated_348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQILGVEAQLLRDAVTAGQGLTVNTAALVTL
DGQHHTVDLTLIPLDDEAELSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
LKEFTTLIIEQADRLRNLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILLRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHSGRIDCLSSAGHTEFIISLPILGAK
>Mature_348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQILGVEAQLLRDAVTAGQGLTVNTAALVTL
DGQHHTVDLTLIPLDDEAELSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
LKEFTTLIIEQADRLRNLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILLRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTGREQGSGLGLSIAHNIA
RLHSGRIDCLSSAGHTEFIISLPILGAK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=343, Percent_Identity=53.6443148688047, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1788549, Length=348, Percent_Identity=26.4367816091954, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790436, Length=253, Percent_Identity=28.8537549407115, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790861, Length=293, Percent_Identity=25.938566552901, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082128, Length=257, Percent_Identity=28.7937743190661, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=26.8398268398268, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=261, Percent_Identity=24.904214559387, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38446; Mature: 38446

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQILGVEAQLL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEHHHHHHHH
RDAVTAGQGLTVNTAALVTLDGQHHTVDLTLIPLDDEAELSLLELRQVDQQRRIHQQLSQ
HHHHHCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPALKEFTTLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
NILQNAVQALEHTGGEILLRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYP
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNIARLHSGRIDCLSSAGHTEFIISLPILGAK
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQILGVEAQLL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEHHHHHHHH
RDAVTAGQGLTVNTAALVTLDGQHHTVDLTLIPLDDEAELSLLELRQVDQQRRIHQQLSQ
HHHHHCCCCCEEEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPALKEFTTLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
NILQNAVQALEHTGGEILLRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYP
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNIARLHSGRIDCLSSAGHTEFIISLPILGAK
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]