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The map label for this gene is cofH [H]

Identifier: 159901219

GI number: 159901219

Start: 6012859

End: 6014058

Strand: Direct

Name: cofH [H]

Synonym: Haur_4707

Alternate gene names: 159901219

Gene position: 6012859-6014058 (Clockwise)

Preceding gene: 159901215

Following gene: 159901220

Centisome position: 94.74

GC content: 49.5

Gene sequence:

>1200_bases
ATGAATGTACAATCGCTTCTTGAAAAAGCAGTCAATGGTGAGCGTCTTTCGGCTGAGGAAGGGCTGTTTTTGTATCGTGA
AGCTGACCTGCTGGATTTAGGCATGGCTGCTGATGCGATTCGCCAACGGCTTGTGCCCGGAAACTTAGTGACGTATCTTG
TTGATCGCAACGTCAATTATAGTAATGTTTGCACGACCAATTGCCAATTCTGTGGTTTTTATCGCCCATTGGGTCACCCA
GAATCCTATGTGCAAACCTACGAAGAAATTGGTGCTCGTTTAGCCGAGCTTGTCGAATATGGTGGAACCCGCGTGCTGAT
GCAGGGTGGCCATCATCCTGAATTGCGGCTTTCGTGGTATACCGGCTTACTGAGCTATTTGCGCGAACATTATCCTAGCA
TCGAGCTTGATTGCTTCTCGCCTTCAGAAATCGATAATCTGGCTAAGGTCGAGAACATGAGCATTCGCGAGGTGTTGATT
GCGCTGAAGGAAGCTGGCTTGGCTGGCGTGCCTGGCGGCGGCGCTGAAATTCTCGATGATGAAGTGCGCCAACGGGTTAG
CCCACTCAAGCAAGATGCTGCTGGTTGGCTCGAAGTTCAGGGTACAGCGCAATCGCTGGGCATGGCCACCACCGCAACTA
TGGTGATTGGCTTCGGCGAGTCGCTTGAACAACGTATGAATCACCTTATGAAATTGCGTGATTTGCAGGATGTTTCGTTG
CGCGAGTATAACAATGGCTTTATTGCCTTTATTTCGTGGACCGTCCAGAAATCGGAGTTGACCTCGTTGGGTCGCTCGAA
GTTCTCGGACGACTACGGGGCAACCGCTGCCGAATATTTGCGCCACACCGCGCTTGCCCGAATTATGCTCGATAACTTTA
ATCATATTCAGGCTTCATGGGTAACTCAAGGACCAAAGATTGGCCAAGTTTCGCTGAAGTTTGGCATCGATGATTTTGGC
TCGACCATGCTTGAGGAAAATGTGGTTTCCAACGCCGCTCACGACACCTACCAATGTATGGGCGAACGCGAAATCCACGG
TTTTATTCGCGATGCTGGCTTTATTCCAGCCAAACGCGATACCCACTACAACTTGATTCAAGCTTTTGAAGACCCCAAAG
ATAGCGAAAACGTGCCATCGATGGGCATCAAACAGCCACGTCAAGCCAAGCAGGTCGAAATTCCATTAATGGTTAAATAG

Upstream 100 bases:

>100_bases
CAAAGCACCCTGAAATCGCCGTGTGAAATATGATACAATTGAAGCGACGGACTGCATTGAGTCAGCCCGCCACCAAAACC
AACGGGGGGCTTTTTTAATT

Downstream 100 bases:

>100_bases
GTTTTTCGGCGAACGCTATGGATGCTTGGTCGTCCATAGCGTTTGTATTTAAGCAACGAATGGCTGTGTGCGATGTTTAC
CTGCCGCCATATTTCCAAGC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNVQSLLEKAVNGERLSAEEGLFLYREADLLDLGMAADAIRQRLVPGNLVTYLVDRNVNYSNVCTTNCQFCGFYRPLGHP
ESYVQTYEEIGARLAELVEYGGTRVLMQGGHHPELRLSWYTGLLSYLREHYPSIELDCFSPSEIDNLAKVENMSIREVLI
ALKEAGLAGVPGGGAEILDDEVRQRVSPLKQDAAGWLEVQGTAQSLGMATTATMVIGFGESLEQRMNHLMKLRDLQDVSL
REYNNGFIAFISWTVQKSELTSLGRSKFSDDYGATAAEYLRHTALARIMLDNFNHIQASWVTQGPKIGQVSLKFGIDDFG
STMLEENVVSNAAHDTYQCMGEREIHGFIRDAGFIPAKRDTHYNLIQAFEDPKDSENVPSMGIKQPRQAKQVEIPLMVK

Sequences:

>Translated_399_residues
MNVQSLLEKAVNGERLSAEEGLFLYREADLLDLGMAADAIRQRLVPGNLVTYLVDRNVNYSNVCTTNCQFCGFYRPLGHP
ESYVQTYEEIGARLAELVEYGGTRVLMQGGHHPELRLSWYTGLLSYLREHYPSIELDCFSPSEIDNLAKVENMSIREVLI
ALKEAGLAGVPGGGAEILDDEVRQRVSPLKQDAAGWLEVQGTAQSLGMATTATMVIGFGESLEQRMNHLMKLRDLQDVSL
REYNNGFIAFISWTVQKSELTSLGRSKFSDDYGATAAEYLRHTALARIMLDNFNHIQASWVTQGPKIGQVSLKFGIDDFG
STMLEENVVSNAAHDTYQCMGEREIHGFIRDAGFIPAKRDTHYNLIQAFEDPKDSENVPSMGIKQPRQAKQVEIPLMVK
>Mature_399_residues
MNVQSLLEKAVNGERLSAEEGLFLYREADLLDLGMAADAIRQRLVPGNLVTYLVDRNVNYSNVCTTNCQFCGFYRPLGHP
ESYVQTYEEIGARLAELVEYGGTRVLMQGGHHPELRLSWYTGLLSYLREHYPSIELDCFSPSEIDNLAKVENMSIREVLI
ALKEAGLAGVPGGGAEILDDEVRQRVSPLKQDAAGWLEVQGTAQSLGMATTATMVIGFGESLEQRMNHLMKLRDLQDVSL
REYNNGFIAFISWTVQKSELTSLGRSKFSDDYGATAAEYLRHTALARIMLDNFNHIQASWVTQGPKIGQVSLKFGIDDFG
STMLEENVVSNAAHDTYQCMGEREIHGFIRDAGFIPAKRDTHYNLIQAFEDPKDSENVPSMGIKQPRQAKQVEIPLMVK

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44565; Mature: 44565

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQSLLEKAVNGERLSAEEGLFLYREADLLDLGMAADAIRQRLVPGNLVTYLVDRNVNY
CCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
SNVCTTNCQFCGFYRPLGHPESYVQTYEEIGARLAELVEYGGTRVLMQGGHHPELRLSWY
CCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHH
TGLLSYLREHYPSIELDCFSPSEIDNLAKVENMSIREVLIALKEAGLAGVPGGGAEILDD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
EVRQRVSPLKQDAAGWLEVQGTAQSLGMATTATMVIGFGESLEQRMNHLMKLRDLQDVSL
HHHHHHHHHHHHCCCEEEEECCHHHHCHHHHHHHEEECCHHHHHHHHHHHHHHCCCCCHH
REYNNGFIAFISWTVQKSELTSLGRSKFSDDYGATAAEYLRHTALARIMLDNFNHIQASW
HHCCCCEEEEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
VTQGPKIGQVSLKFGIDDFGSTMLEENVVSNAAHDTYQCMGEREIHGFIRDAGFIPAKRD
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCC
THYNLIQAFEDPKDSENVPSMGIKQPRQAKQVEIPLMVK
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MNVQSLLEKAVNGERLSAEEGLFLYREADLLDLGMAADAIRQRLVPGNLVTYLVDRNVNY
CCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
SNVCTTNCQFCGFYRPLGHPESYVQTYEEIGARLAELVEYGGTRVLMQGGHHPELRLSWY
CCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHH
TGLLSYLREHYPSIELDCFSPSEIDNLAKVENMSIREVLIALKEAGLAGVPGGGAEILDD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
EVRQRVSPLKQDAAGWLEVQGTAQSLGMATTATMVIGFGESLEQRMNHLMKLRDLQDVSL
HHHHHHHHHHHHCCCEEEEECCHHHHCHHHHHHHEEECCHHHHHHHHHHHHHHCCCCCHH
REYNNGFIAFISWTVQKSELTSLGRSKFSDDYGATAAEYLRHTALARIMLDNFNHIQASW
HHCCCCEEEEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
VTQGPKIGQVSLKFGIDDFGSTMLEENVVSNAAHDTYQCMGEREIHGFIRDAGFIPAKRD
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCC
THYNLIQAFEDPKDSENVPSMGIKQPRQAKQVEIPLMVK
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]