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The map label for this gene is gacA [H]

Identifier: 159901124

GI number: 159901124

Start: 5897797

End: 5899050

Strand: Direct

Name: gacA [H]

Synonym: Haur_4612

Alternate gene names: 159901124

Gene position: 5897797-5899050 (Clockwise)

Preceding gene: 159901123

Following gene: 159901125

Centisome position: 92.93

GC content: 48.25

Gene sequence:

>1254_bases
ATGGCTGAGTCCGATAAAATTCGCGTACTAATTGTTGACGATATTGCAGATACTCGTGATAACCTCGAAAAATTATTGTT
TTTTGAGAAAGATATGCAAGTTGTCGGCAAAGCAGCAACGGGGCGCGAGGCCGTGACGCAGGCCAAGCAAATTCAACCCG
ATGTTGTCTTGATGGATATCAACATGCCCGATATGGATGGCATTGCAGCAACTGAGGCAATCATGGCGCAGGTGCCAAAT
ACGCAAGTCATTATGATGAGCGTGCAAGGCGAAACCGATTACCTGCGCCGTGCAATGTTGGCTGGTGCGCGTCAGTTTCT
CACGAAACCAGTTGGTGGCGATGAACTCGCCAGTAGCATCCGCGAAGTTTATCGCTTGCAGCAAACCCAACGACGCTTTG
TGGTAGCGGCCCAACAGGTTGAAGAACATGATCAATCAACTGGCCAAATTATTGCTGTCTATAGCCCCAAAGGTGGCACT
GGCAAGAGTGCAATTGCCTCAAATTTGGCGGTTGCATTAAAATTATTGCCTGGTAATCGTAAAATTTGTTTGGTCGATGC
AAGCTTGCTGTTTGGCGATATTGCGGTGATGTTCAACATCAACAGTTCCAAAACGATCAACGATCTCACCTCGCGGATTG
ACGATCTTGATAAGGATTTGCTGAATGATGTGATGACGACCCATGCCTCACAGATCAAGGTGTTGTTGGCTCCGGCTAAC
CCACAAATGGGCGAACTAGTCACGGCTGATCATGTCCGAACAGTGCTTGAAGCCCTGCGGCGAGAGTATGATTATGTGGT
GGTCGATACCCAATCATCCTTCCAAGATCAAACCATGGCCGTATTAGATGCAGCCCATCGGATTGTTTTGTTGATGACGA
TGGAACTCTCGTCGATTAAGAATATTCGCCAGTTTTTGGAAGTGGCCGAGTTGCTTGGCTACAACGATGAAAAACTGGTG
TTGGTCTTGAACAAAGCCGATGCTAAATTTGGCATTCGGGTCGATCAAGTCGAGGCCAATATTCAACATAAGGTTGCGGC
CCAAATTGGCAATGCGCCATTTGAAATGGTCAATGCGATCAACCGCGGCGTACCTTTAATTATCGATCAGCCCCGGCATC
AAATTTCAATCGATGTGGCCAACCTAGCGTATCTCATCTCGGGAACAACCCGTACCAGCCGCGAAGGCGCACGCCCGCAA
CAACCGAAAAAAGAAGAACCGAAGGGTCTCTTCGCCCGATTGACCAAACGTTAG

Upstream 100 bases:

>100_bases
GCAGTTGTTATTCCATTCCGATTACCAAACGCACCACAACAACAACCATTGCCAACAGCAGTGCCAACTCAAACGCCTTA
ATACAACCAAGGAGAATTGC

Downstream 100 bases:

>100_bases
ACGCATGGAGGGTGGCTTATGTCGCTCCTAAAGCGTATTGCAGGAAATACGTCCCCATCCTCGGCTTCCGAACCTACAGC
AGCAGCACAATCGAGTGCTG

Product: response regulator receiver protein

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAESDKIRVLIVDDIADTRDNLEKLLFFEKDMQVVGKAATGREAVTQAKQIQPDVVLMDINMPDMDGIAATEAIMAQVPN
TQVIMMSVQGETDYLRRAMLAGARQFLTKPVGGDELASSIREVYRLQQTQRRFVVAAQQVEEHDQSTGQIIAVYSPKGGT
GKSAIASNLAVALKLLPGNRKICLVDASLLFGDIAVMFNINSSKTINDLTSRIDDLDKDLLNDVMTTHASQIKVLLAPAN
PQMGELVTADHVRTVLEALRREYDYVVVDTQSSFQDQTMAVLDAAHRIVLLMTMELSSIKNIRQFLEVAELLGYNDEKLV
LVLNKADAKFGIRVDQVEANIQHKVAAQIGNAPFEMVNAINRGVPLIIDQPRHQISIDVANLAYLISGTTRTSREGARPQ
QPKKEEPKGLFARLTKR

Sequences:

>Translated_417_residues
MAESDKIRVLIVDDIADTRDNLEKLLFFEKDMQVVGKAATGREAVTQAKQIQPDVVLMDINMPDMDGIAATEAIMAQVPN
TQVIMMSVQGETDYLRRAMLAGARQFLTKPVGGDELASSIREVYRLQQTQRRFVVAAQQVEEHDQSTGQIIAVYSPKGGT
GKSAIASNLAVALKLLPGNRKICLVDASLLFGDIAVMFNINSSKTINDLTSRIDDLDKDLLNDVMTTHASQIKVLLAPAN
PQMGELVTADHVRTVLEALRREYDYVVVDTQSSFQDQTMAVLDAAHRIVLLMTMELSSIKNIRQFLEVAELLGYNDEKLV
LVLNKADAKFGIRVDQVEANIQHKVAAQIGNAPFEMVNAINRGVPLIIDQPRHQISIDVANLAYLISGTTRTSREGARPQ
QPKKEEPKGLFARLTKR
>Mature_416_residues
AESDKIRVLIVDDIADTRDNLEKLLFFEKDMQVVGKAATGREAVTQAKQIQPDVVLMDINMPDMDGIAATEAIMAQVPNT
QVIMMSVQGETDYLRRAMLAGARQFLTKPVGGDELASSIREVYRLQQTQRRFVVAAQQVEEHDQSTGQIIAVYSPKGGTG
KSAIASNLAVALKLLPGNRKICLVDASLLFGDIAVMFNINSSKTINDLTSRIDDLDKDLLNDVMTTHASQIKVLLAPANP
QMGELVTADHVRTVLEALRREYDYVVVDTQSSFQDQTMAVLDAAHRIVLLMTMELSSIKNIRQFLEVAELLGYNDEKLVL
VLNKADAKFGIRVDQVEANIQHKVAAQIGNAPFEMVNAINRGVPLIIDQPRHQISIDVANLAYLISGTTRTSREGARPQQ
PKKEEPKGLFARLTKR

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=136, Percent_Identity=36.0294117647059, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=35.7142857142857, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790102, Length=118, Percent_Identity=34.7457627118644, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 46062; Mature: 45931

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESDKIRVLIVDDIADTRDNLEKLLFFEKDMQVVGKAATGREAVTQAKQIQPDVVLMDI
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEC
NMPDMDGIAATEAIMAQVPNTQVIMMSVQGETDYLRRAMLAGARQFLTKPVGGDELASSI
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
REVYRLQQTQRRFVVAAQQVEEHDQSTGQIIAVYSPKGGTGKSAIASNLAVALKLLPGNR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHEEEEEECCCCC
KICLVDASLLFGDIAVMFNINSSKTINDLTSRIDDLDKDLLNDVMTTHASQIKVLLAPAN
EEEEEEHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PQMGELVTADHVRTVLEALRREYDYVVVDTQSSFQDQTMAVLDAAHRIVLLMTMELSSIK
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEHHHHHHHH
NIRQFLEVAELLGYNDEKLVLVLNKADAKFGIRVDQVEANIQHKVAAQIGNAPFEMVNAI
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHH
NRGVPLIIDQPRHQISIDVANLAYLISGTTRTSREGARPQQPKKEEPKGLFARLTKR
HCCCCEEEECCCCEEEEEHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
AESDKIRVLIVDDIADTRDNLEKLLFFEKDMQVVGKAATGREAVTQAKQIQPDVVLMDI
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEC
NMPDMDGIAATEAIMAQVPNTQVIMMSVQGETDYLRRAMLAGARQFLTKPVGGDELASSI
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
REVYRLQQTQRRFVVAAQQVEEHDQSTGQIIAVYSPKGGTGKSAIASNLAVALKLLPGNR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHEEEEEECCCCC
KICLVDASLLFGDIAVMFNINSSKTINDLTSRIDDLDKDLLNDVMTTHASQIKVLLAPAN
EEEEEEHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PQMGELVTADHVRTVLEALRREYDYVVVDTQSSFQDQTMAVLDAAHRIVLLMTMELSSIK
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEHHHHHHHH
NIRQFLEVAELLGYNDEKLVLVLNKADAKFGIRVDQVEANIQHKVAAQIGNAPFEMVNAI
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHH
NRGVPLIIDQPRHQISIDVANLAYLISGTTRTSREGARPQQPKKEEPKGLFARLTKR
HCCCCEEEECCCCEEEEEHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]