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The map label for this gene is gca [H]

Identifier: 159901079

GI number: 159901079

Start: 5845049

End: 5846026

Strand: Reverse

Name: gca [H]

Synonym: Haur_4567

Alternate gene names: 159901079

Gene position: 5846026-5845049 (Counterclockwise)

Preceding gene: 159901081

Following gene: 159901078

Centisome position: 92.11

GC content: 49.59

Gene sequence:

>978_bases
ATGACCAATAAAGTCGCAGTGGTGACCGGAATTACCGGCCAAGATGGTTCGTATTTAGCTGAACATTTGCTGGCTCAAGG
CTACCGCGTAGTCGGGATGCTGCGGCGTTCCAGCACAGTTAATTTCGAGCGCATCAAACATATTCAAGATGATATCGAAC
TGGTCACGGGCGATTTGCTCGATGAAGTCTCGTTAATTAATATGCTGCAAACCTATCGACCTTCCGAGGTTTATAACTTA
GCAGCCCAAAGCTTTGTCCAAACGTCGTGGTCGCAACCAGTCTTTACTGGCGAAGCTACGGCCTTGGGCGTGACGCGGAT
TCTTGACGCGGTGCGCACGGTCGATCCCTCGATTCGCTTTTATCAAGCGAGTTCGTCGGAGATGTTTGGCAAAGTGGTTG
AAGTGCCGCAAACCGAAAATACGCCATTTTACCCACGCTCGCCGTATGGGGTTGCCAAAGTTTATGGCCACTGGATCACC
GTGAATTATCGTGAAAGCTACAATATGTTTGCTTGCTCAGGCATTTTGTTCAATCACGAAAGCCCACGCCGTGGCCGCGA
GTTTGTCACCCGCAAAATCACCGATGCAGTAGCGCGAATTAAGCTTGGCTTAGCGACTGAATTACGTTTGGGCAATCTCG
AAGCCCAGCGCGATTGGGGCTTTGCAGGCGATTATGTCAAAGCCATGTATTTGATGTTGCAACAAGATCAGCCCGATGAT
TTTGTGGTTTCAACTGGCGAAACCCACTCAGTACGCCGCTTCTGCGAGATCGCGTTTGGCCATGTTGGCTTAAATTATGC
CGACTATGTAAAAATCGACGAACGCTTTATGCGGCCTGCCGAAGTGGACTTGTTGATTGGCGACCCAGCCAAGGCCAAGC
GTATTTTAGGTTGGCAACCAGAAACGCCCTTCGAGCAACTGGTGACCATGATGGTCGAAGCTGACCTCGAATTATTGAAA
CATGAATACCGTATCTAA

Upstream 100 bases:

>100_bases
ACATTCGCCAAGGTGATTGCTAAGCCACTTAGCAGCCACCCCAACCATATCGAGCTATGCTAGAACGATATGAATCTAGC
CTAGATCGAATGAGGATAGC

Downstream 100 bases:

>100_bases
AGGCAAAAGGCAGAAGTAAAAAGTCAAAAAGTACGATTACTGAATGAAGATGGCTCTTTGATTTTCATTGCGGCAGAAGT
AAGAAGCCAAAAATAGATCT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTNKVAVVTGITGQDGSYLAEHLLAQGYRVVGMLRRSSTVNFERIKHIQDDIELVTGDLLDEVSLINMLQTYRPSEVYNL
AAQSFVQTSWSQPVFTGEATALGVTRILDAVRTVDPSIRFYQASSSEMFGKVVEVPQTENTPFYPRSPYGVAKVYGHWIT
VNYRESYNMFACSGILFNHESPRRGREFVTRKITDAVARIKLGLATELRLGNLEAQRDWGFAGDYVKAMYLMLQQDQPDD
FVVSTGETHSVRRFCEIAFGHVGLNYADYVKIDERFMRPAEVDLLIGDPAKAKRILGWQPETPFEQLVTMMVEADLELLK
HEYRI

Sequences:

>Translated_325_residues
MTNKVAVVTGITGQDGSYLAEHLLAQGYRVVGMLRRSSTVNFERIKHIQDDIELVTGDLLDEVSLINMLQTYRPSEVYNL
AAQSFVQTSWSQPVFTGEATALGVTRILDAVRTVDPSIRFYQASSSEMFGKVVEVPQTENTPFYPRSPYGVAKVYGHWIT
VNYRESYNMFACSGILFNHESPRRGREFVTRKITDAVARIKLGLATELRLGNLEAQRDWGFAGDYVKAMYLMLQQDQPDD
FVVSTGETHSVRRFCEIAFGHVGLNYADYVKIDERFMRPAEVDLLIGDPAKAKRILGWQPETPFEQLVTMMVEADLELLK
HEYRI
>Mature_324_residues
TNKVAVVTGITGQDGSYLAEHLLAQGYRVVGMLRRSSTVNFERIKHIQDDIELVTGDLLDEVSLINMLQTYRPSEVYNLA
AQSFVQTSWSQPVFTGEATALGVTRILDAVRTVDPSIRFYQASSSEMFGKVVEVPQTENTPFYPRSPYGVAKVYGHWITV
NYRESYNMFACSGILFNHESPRRGREFVTRKITDAVARIKLGLATELRLGNLEAQRDWGFAGDYVKAMYLMLQQDQPDDF
VVSTGETHSVRRFCEIAFGHVGLNYADYVKIDERFMRPAEVDLLIGDPAKAKRILGWQPETPFEQLVTMMVEADLELLKH
EYRI

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=51.3119533527697, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=50.2824858757062, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1788353, Length=340, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994969, Length=278, Percent_Identity=24.1007194244604, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=50.8720930232558, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=51.1627906976744, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=51.1627906976744, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=51.1627906976744, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=51.1627906976744, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=51.1627906976744, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24158427, Length=345, Percent_Identity=52.1739130434783, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36899; Mature: 36767

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVAVVTGITGQDGSYLAEHLLAQGYRVVGMLRRSSTVNFERIKHIQDDIELVTGDLL
CCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DEVSLINMLQTYRPSEVYNLAAQSFVQTSWSQPVFTGEATALGVTRILDAVRTVDPSIRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
YQASSSEMFGKVVEVPQTENTPFYPRSPYGVAKVYGHWITVNYRESYNMFACSGILFNHE
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCEEEEEEEEECCC
SPRRGREFVTRKITDAVARIKLGLATELRLGNLEAQRDWGFAGDYVKAMYLMLQQDQPDD
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FVVSTGETHSVRRFCEIAFGHVGLNYADYVKIDERFMRPAEVDLLIGDPAKAKRILGWQP
EEEECCCHHHHHHHHHHHHHHCCCCHHHHEEHHHHHCCCCCEEEEECCCHHHHHHCCCCC
ETPFEQLVTMMVEADLELLKHEYRI
CCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNKVAVVTGITGQDGSYLAEHLLAQGYRVVGMLRRSSTVNFERIKHIQDDIELVTGDLL
CCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
DEVSLINMLQTYRPSEVYNLAAQSFVQTSWSQPVFTGEATALGVTRILDAVRTVDPSIRF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
YQASSSEMFGKVVEVPQTENTPFYPRSPYGVAKVYGHWITVNYRESYNMFACSGILFNHE
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCEEEEEEEEECCC
SPRRGREFVTRKITDAVARIKLGLATELRLGNLEAQRDWGFAGDYVKAMYLMLQQDQPDD
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FVVSTGETHSVRRFCEIAFGHVGLNYADYVKIDERFMRPAEVDLLIGDPAKAKRILGWQP
EEEECCCHHHHHHHHHHHHHHCCCCHHHHEEHHHHHCCCCCEEEEECCCHHHHHHCCCCC
ETPFEQLVTMMVEADLELLKHEYRI
CCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]