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The map label for this gene is leuD

Identifier: 159900953

GI number: 159900953

Start: 5680944

End: 5681534

Strand: Reverse

Name: leuD

Synonym: Haur_4440

Alternate gene names: 159900953

Gene position: 5681534-5680944 (Counterclockwise)

Preceding gene: 159900954

Following gene: 159900952

Centisome position: 89.52

GC content: 47.55

Gene sequence:

>591_bases
ATGCAACCAATTAATACTTTTCAGGCCAAAGCAGTGGCCTTACCAATTGAAAATATCGACACCGATCAGATCATTCCGGC
CCGCTATTTAAAGGTTACCGATAAAAATGGCTTGGGTGAAGCATTGTTTACCGACTGGCGCGGTGAGCCAGATTTTGTGT
TGAACCAACCCTATGCTCAAGGCGCTGGCGTGCTAATTGCAGGCCATAACTTTGGCTGTGGATCTTCGCGTGAACATGCA
CCGTGGGCCTTGCAAGGCTTTGGCTTCCAAGCAGTGATCAGCACCTATTTTGCTGATATTTTCAAAGGCAATGCTTTGAA
AAATGGCCTTTTGCCAATCGTGGTCGATGCACCAACCTTGGCTCGTTTGACCGAGCAATGCCTAGCTAACCAAACCATCG
ATGTCAGCGTTGACCTTGAAAACCAACAAGTGCATGTGGCAGGCGAAACCATCAGCTTCCCAATTGATGCCTTCTCCAAA
CATTGCTTATTGCATGGAGTCGATCAATTAGGCTATATTCAAGCCCAAGAAACTGCAATCCAAGCCTATGAAGCTAGCCA
TGCCGCCCGCGTCAATACGGTTGGAGCATAA

Upstream 100 bases:

>100_bases
CAAAAATCAAAATAATCTGTGGTTTTGACTTTTGCCTTTGATACTGTTTATTTTGCCTTTTGAACTTTGACTTTTGACTT
TACATATGGGGTTTTTAAAA

Downstream 100 bases:

>100_bases
TAAGGCTTGGTAGAGTGACGTTCCCTCATCCCCAACCCTTCTCCCATGCGTGAGAGAAGGGAGCTTTGGCTTTGTGGTTC
CCCCCTCGCTCGCAGGCGGG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MQPINTFQAKAVALPIENIDTDQIIPARYLKVTDKNGLGEALFTDWRGEPDFVLNQPYAQGAGVLIAGHNFGCGSSREHA
PWALQGFGFQAVISTYFADIFKGNALKNGLLPIVVDAPTLARLTEQCLANQTIDVSVDLENQQVHVAGETISFPIDAFSK
HCLLHGVDQLGYIQAQETAIQAYEASHAARVNTVGA

Sequences:

>Translated_196_residues
MQPINTFQAKAVALPIENIDTDQIIPARYLKVTDKNGLGEALFTDWRGEPDFVLNQPYAQGAGVLIAGHNFGCGSSREHA
PWALQGFGFQAVISTYFADIFKGNALKNGLLPIVVDAPTLARLTEQCLANQTIDVSVDLENQQVHVAGETISFPIDAFSK
HCLLHGVDQLGYIQAQETAIQAYEASHAARVNTVGA
>Mature_196_residues
MQPINTFQAKAVALPIENIDTDQIIPARYLKVTDKNGLGEALFTDWRGEPDFVLNQPYAQGAGVLIAGHNFGCGSSREHA
PWALQGFGFQAVISTYFADIFKGNALKNGLLPIVVDAPTLARLTEQCLANQTIDVSVDLENQQVHVAGETISFPIDAFSK
HCLLHGVDQLGYIQAQETAIQAYEASHAARVNTVGA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=189, Percent_Identity=46.5608465608466, Blast_Score=173, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=40.2010050251256, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_HERA2 (A9AZ32)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547200.1
- ProteinModelPortal:   A9AZ32
- SMR:   A9AZ32
- GeneID:   5736291
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4440
- HOGENOM:   HBG304838
- OMA:   DEISITM
- BioCyc:   HAUR316274:HAUR_4440-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21164; Mature: 21164

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPINTFQAKAVALPIENIDTDQIIPARYLKVTDKNGLGEALFTDWRGEPDFVLNQPYAQ
CCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCEEECCCCCC
GAGVLIAGHNFGCGSSREHAPWALQGFGFQAVISTYFADIFKGNALKNGLLPIVVDAPTL
CCEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHH
ARLTEQCLANQTIDVSVDLENQQVHVAGETISFPIDAFSKHCLLHGVDQLGYIQAQETAI
HHHHHHHHCCCCEEEEEECCCCEEEEECCEEECCHHHHHHHHHHHCCCHHCEEEHHHHHH
QAYEASHAARVNTVGA
HHHHCCCCEEEEECCC
>Mature Secondary Structure
MQPINTFQAKAVALPIENIDTDQIIPARYLKVTDKNGLGEALFTDWRGEPDFVLNQPYAQ
CCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCEEECCCCCC
GAGVLIAGHNFGCGSSREHAPWALQGFGFQAVISTYFADIFKGNALKNGLLPIVVDAPTL
CCEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHH
ARLTEQCLANQTIDVSVDLENQQVHVAGETISFPIDAFSKHCLLHGVDQLGYIQAQETAI
HHHHHHHHCCCCEEEEEECCCCEEEEECCEEECCHHHHHHHHHHHCCCHHCEEEHHHHHH
QAYEASHAARVNTVGA
HHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA