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The map label for this gene is leuB [H]

Identifier: 159900952

GI number: 159900952

Start: 5679707

End: 5680780

Strand: Reverse

Name: leuB [H]

Synonym: Haur_4439

Alternate gene names: 159900952

Gene position: 5680780-5679707 (Counterclockwise)

Preceding gene: 159900953

Following gene: 159900944

Centisome position: 89.51

GC content: 55.68

Gene sequence:

>1074_bases
ATGCAGGCAACAATTGCTTTATTACCAGGCGATGGCATCGGCCCCGAAGTTGTCGCTGAGGGGGTTGAGGTGCTCAAGGC
CGTCGGTGAACGCTACGGCCATAGTTTTAGTTTCAGCGAAGGCTTAATCGGTGGTTGTGCAATCGATGCCCACGGCACAG
CGTTGCCCGAAGCCACAATCGAAATCTGCCGAGCCGCCGATGCCGTGCTGCTGGGCGCGGTTGGCGGCCCCAAGTGGGAT
GATCCCAAGGCCAAAGTTCGACCAGAACAAGGCTTGTTGGGCATTCGCAAAACCTTGGGCTTGTATGCCAATTTGCGGCC
TGTGACCGTCACGCCCGCCTTAGCTAGCCGCTCACCATTGCGCCCCGATTTGGTCGAAGGGGTCGATTTGTTGGTAGTGC
GCGAATTAACTGGCGGGATTTACTTCGGCGATAAAACCTGTGTCTACATTCGCCCCGACGAAGAACAAGCTGTCGATACC
TGTGTCTATACCACCAGCGAAATCGAGCGCGTGGTGCGGCGGGCTTGTGAATTGGCACGGACTCGCCGTGGCAAAGTTAC
CTCGGTCGATAAAGCCAATGTCTTGGAAACCTCACGCTTATGGCGGCGCGTCGCCAGCCGAGTGGTTGCCGAAGAATTCC
CTGATATTCAGCTTGAGCATATTTTGGTCGATGCCTGTGCGATGCACTTGTTGCGCCGCCCTGCCGACTTCGATGTGATT
GTGACTGAAAATATGTTTGGCGATATTCTGACCGACGAAGCGTCGATGTTGGCTGGCTCGTTGGGCTTGTTGCCATCGGC
CTCGCTTGGTGCGGGCAAAGCTGGTTTGTACGAGCCAATTCACGGCAGCGCTCCCGATATTGCTGGCAAAGGCGTGGCCA
ATCCACTGGCGACAATTTTGAGCGTAGCCTTGCTGTTGCGCTATTCCTTGGGCTTAGATACCGAGGCTGAGGCAGTCGAA
AAAGCCGTATATGCAACGATCGATGCAGGCATTTTAACTGGCGATTTAGCGCCTGCAGGCCAAGGCCGCTCAACCAAAGA
AGTTGGGGCAGCGGTGTTGGAACGTTTGGGCTAA

Upstream 100 bases:

>100_bases
CTTTGGCTTTGTGGTTCCCCCCTCGCTCGCAGGCGGGAGAGGGGGCTAGGGGGTGAGGGAACGCCACCGCTTGTTGGTTA
AATTAGCAAGGAGTGTTTTG

Downstream 100 bases:

>100_bases
GCATCAATTACGCCCCTGGTTCAATGGCGAGCTAGGGGCGTTGGCATTTTAGGCTTGTTACCCCAGCAATTCGTATAACC
CCAAAACGCCCAAACCAGTG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQATIALLPGDGIGPEVVAEGVEVLKAVGERYGHSFSFSEGLIGGCAIDAHGTALPEATIEICRAADAVLLGAVGGPKWD
DPKAKVRPEQGLLGIRKTLGLYANLRPVTVTPALASRSPLRPDLVEGVDLLVVRELTGGIYFGDKTCVYIRPDEEQAVDT
CVYTTSEIERVVRRACELARTRRGKVTSVDKANVLETSRLWRRVASRVVAEEFPDIQLEHILVDACAMHLLRRPADFDVI
VTENMFGDILTDEASMLAGSLGLLPSASLGAGKAGLYEPIHGSAPDIAGKGVANPLATILSVALLLRYSLGLDTEAEAVE
KAVYATIDAGILTGDLAPAGQGRSTKEVGAAVLERLG

Sequences:

>Translated_357_residues
MQATIALLPGDGIGPEVVAEGVEVLKAVGERYGHSFSFSEGLIGGCAIDAHGTALPEATIEICRAADAVLLGAVGGPKWD
DPKAKVRPEQGLLGIRKTLGLYANLRPVTVTPALASRSPLRPDLVEGVDLLVVRELTGGIYFGDKTCVYIRPDEEQAVDT
CVYTTSEIERVVRRACELARTRRGKVTSVDKANVLETSRLWRRVASRVVAEEFPDIQLEHILVDACAMHLLRRPADFDVI
VTENMFGDILTDEASMLAGSLGLLPSASLGAGKAGLYEPIHGSAPDIAGKGVANPLATILSVALLLRYSLGLDTEAEAVE
KAVYATIDAGILTGDLAPAGQGRSTKEVGAAVLERLG
>Mature_357_residues
MQATIALLPGDGIGPEVVAEGVEVLKAVGERYGHSFSFSEGLIGGCAIDAHGTALPEATIEICRAADAVLLGAVGGPKWD
DPKAKVRPEQGLLGIRKTLGLYANLRPVTVTPALASRSPLRPDLVEGVDLLVVRELTGGIYFGDKTCVYIRPDEEQAVDT
CVYTTSEIERVVRRACELARTRRGKVTSVDKANVLETSRLWRRVASRVVAEEFPDIQLEHILVDACAMHLLRRPADFDVI
VTENMFGDILTDEASMLAGSLGLLPSASLGAGKAGLYEPIHGSAPDIAGKGVANPLATILSVALLLRYSLGLDTEAEAVE
KAVYATIDAGILTGDLAPAGQGRSTKEVGAAVLERLG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=34.1176470588235, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=29.2613636363636, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178821, Length=324, Percent_Identity=27.1604938271605, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI28178816, Length=324, Percent_Identity=27.1604938271605, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178838, Length=333, Percent_Identity=29.1291291291291, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178819, Length=180, Percent_Identity=30, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI87081683, Length=347, Percent_Identity=52.1613832853026, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788101, Length=345, Percent_Identity=35.3623188405797, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=26.6009852216749, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=32.6815642458101, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=29.5454545454545, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=364, Percent_Identity=30.4945054945055, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=28.1767955801105, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=48.9010989010989, Blast_Score=318, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=37.5, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=367, Percent_Identity=33.5149863760218, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=364, Percent_Identity=30.2197802197802, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=35.5685131195335, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=35.5685131195335, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=30.9248554913295, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=29.0960451977401, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=29.0960451977401, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078635, Length=362, Percent_Identity=27.6243093922652, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078633, Length=362, Percent_Identity=27.6243093922652, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24650122, Length=362, Percent_Identity=27.6243093922652, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078637, Length=362, Percent_Identity=27.6243093922652, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078639, Length=360, Percent_Identity=27.7777777777778, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20130355, Length=225, Percent_Identity=27.5555555555556, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37672; Mature: 37672

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATIALLPGDGIGPEVVAEGVEVLKAVGERYGHSFSFSEGLIGGCAIDAHGTALPEATI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHH
EICRAADAVLLGAVGGPKWDDPKAKVRPEQGLLGIRKTLGLYANLRPVTVTPALASRSPL
HHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHCCCCC
RPDLVEGVDLLVVRELTGGIYFGDKTCVYIRPDEEQAVDTCVYTTSEIERVVRRACELAR
CCHHHCCHHHEEEHHHHCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRGKVTSVDKANVLETSRLWRRVASRVVAEEFPDIQLEHILVDACAMHLLRRPADFDVI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
VTENMFGDILTDEASMLAGSLGLLPSASLGAGKAGLYEPIHGSAPDIAGKGVANPLATIL
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SVALLLRYSLGLDTEAEAVEKAVYATIDAGILTGDLAPAGQGRSTKEVGAAVLERLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQATIALLPGDGIGPEVVAEGVEVLKAVGERYGHSFSFSEGLIGGCAIDAHGTALPEATI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHH
EICRAADAVLLGAVGGPKWDDPKAKVRPEQGLLGIRKTLGLYANLRPVTVTPALASRSPL
HHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHCCCCC
RPDLVEGVDLLVVRELTGGIYFGDKTCVYIRPDEEQAVDTCVYTTSEIERVVRRACELAR
CCHHHCCHHHEEEHHHHCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRGKVTSVDKANVLETSRLWRRVASRVVAEEFPDIQLEHILVDACAMHLLRRPADFDVI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
VTENMFGDILTDEASMLAGSLGLLPSASLGAGKAGLYEPIHGSAPDIAGKGVANPLATIL
EECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SVALLLRYSLGLDTEAEAVEKAVYATIDAGILTGDLAPAGQGRSTKEVGAAVLERLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA