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The map label for this gene is leuC [H]

Identifier: 159900954

GI number: 159900954

Start: 5681652

End: 5683067

Strand: Reverse

Name: leuC [H]

Synonym: Haur_4441

Alternate gene names: 159900954

Gene position: 5683067-5681652 (Counterclockwise)

Preceding gene: 159900955

Following gene: 159900953

Centisome position: 89.55

GC content: 54.1

Gene sequence:

>1416_bases
ATGGCCAAGACATTATTTGAGAAAATCTGGGATGCCCATGTTGTTCAAGCTGCTGACGCTGAATCGCCAGCAACTTTATA
TATCGATTTGCACTTGGTACACGAAGTAACCTCGCCGCAAGCCTTTACGATGTTGCGCGAACGTGGTTTGACCGTGCGTC
GCCCAGCCCAAACCCTCGCCACCATGGACCATAGCACCCCAACCACGCCACGCGGCGCTGATGGGATTATTCCAGTGACC
GATGCAATTGCTCGCAAACAGCTTGATCAATTGATCAAAAATTGTAGCGATTTTGGCATACCATTGTACAACTTGGGCAC
TGAAAACCAAGGCATTGTGCACGTAATCGGGCCAGAACAAGGCTACACCCAACCAGGTATGACGATTGTGTGTGGCGATT
CGCACACCAGCACCCACGGCGCATTCGGAGCCTTGGCCTTCGGCATTGGCACCAGCGAAGTTGGTCACGTGTTGGCAACT
CAATGTTTGTTGCAGCAAAAACCTCAAACCGCCGAAATTCGCATCGATGGCACGCTGCGTCCAGGTGTGACCGCCAAAGA
CATCATCTTGGCGATTATTGCCAAAATTGGGGTTGGCGGCGGCACGGGCTATGTACTTGAATACACTGGCTCGGCGATTC
GCGCCCTGACCATGGAAGAACGCATGACGATTTGTAATATGTCGATCGAAGGTGGAGCACGGGCTGGCTTGATCGCGCCT
GATGAAACCACCTTTGCTTGGCTGAAAGATCGGCCACATACGCCCAAGGGTGAAGCATGGGATGCTGCGGTCGAATATTG
GCGCACGTTGCCCAGCGACGAAGGCGCAACCTACGATTTGCAAGTTGTCTTGAATGCTGACGAATTGGCTCCGATGATCA
CCTATGGCACGAATCCCGGCATGGGCATTCCGGTCACTGGCAATGTGCCAGCACCCAGCGATTTGGCCGATGATAGCCAA
CGCATGGCCTTGGATAAAGCCTTGAATTACATGGGCTTGCAACCAAACCAATCGCTGATCGGCCAAAAAGTCGATGTAGT
GTTCCTTGGCTCCTGCACCAACTCGCGGATTTCGGATTTACGGGCGGCGGCCAAAGTGATCGAAGGCAAAAAAGTTGCCG
ACGGCCTACGCATGTTGGTCGTGCCTGGCTCACAACAAGTCAAGCGCCAAGCCGAAGCTGAAGGCTTAGACCAAATTTTC
CGCGCGGCAGGCGCTGAATGGCGTGAAGCTGGCTGCTCGATGTGTATTGCCATGAACGGCGATCAACTGCAACCAGGCCA
ATATGCAGTTAGCACCAGCAACCGCAACTTTGAAGGCCGCCAAGGCAAGGGCGGGCGCACCTTCCTCGCCAGCCCGCTGA
CCGCTGCCGCTACCGCAATCAACGGCCATATCGTCGATGTACGCGAAATCTTGTAA

Upstream 100 bases:

>100_bases
AGAACGTTGACCAAATAGCTATAGCCGTGCAGACATTACAAACCGTCCGATAGCCAATAGCCATCAGCCTATAACCTCGC
AATCAAATTTGGAGAACCCA

Downstream 100 bases:

>100_bases
AGTCAAAAGGCAGAAGGCAAAAATCAAAATAATCTGTGGTTTTGACTTTTGCCTTTGATACTGTTTATTTTGCCTTTTGA
ACTTTGACTTTTGACTTTAC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAKTLFEKIWDAHVVQAADAESPATLYIDLHLVHEVTSPQAFTMLRERGLTVRRPAQTLATMDHSTPTTPRGADGIIPVT
DAIARKQLDQLIKNCSDFGIPLYNLGTENQGIVHVIGPEQGYTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLAT
QCLLQQKPQTAEIRIDGTLRPGVTAKDIILAIIAKIGVGGGTGYVLEYTGSAIRALTMEERMTICNMSIEGGARAGLIAP
DETTFAWLKDRPHTPKGEAWDAAVEYWRTLPSDEGATYDLQVVLNADELAPMITYGTNPGMGIPVTGNVPAPSDLADDSQ
RMALDKALNYMGLQPNQSLIGQKVDVVFLGSCTNSRISDLRAAAKVIEGKKVADGLRMLVVPGSQQVKRQAEAEGLDQIF
RAAGAEWREAGCSMCIAMNGDQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATAINGHIVDVREIL

Sequences:

>Translated_471_residues
MAKTLFEKIWDAHVVQAADAESPATLYIDLHLVHEVTSPQAFTMLRERGLTVRRPAQTLATMDHSTPTTPRGADGIIPVT
DAIARKQLDQLIKNCSDFGIPLYNLGTENQGIVHVIGPEQGYTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLAT
QCLLQQKPQTAEIRIDGTLRPGVTAKDIILAIIAKIGVGGGTGYVLEYTGSAIRALTMEERMTICNMSIEGGARAGLIAP
DETTFAWLKDRPHTPKGEAWDAAVEYWRTLPSDEGATYDLQVVLNADELAPMITYGTNPGMGIPVTGNVPAPSDLADDSQ
RMALDKALNYMGLQPNQSLIGQKVDVVFLGSCTNSRISDLRAAAKVIEGKKVADGLRMLVVPGSQQVKRQAEAEGLDQIF
RAAGAEWREAGCSMCIAMNGDQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATAINGHIVDVREIL
>Mature_470_residues
AKTLFEKIWDAHVVQAADAESPATLYIDLHLVHEVTSPQAFTMLRERGLTVRRPAQTLATMDHSTPTTPRGADGIIPVTD
AIARKQLDQLIKNCSDFGIPLYNLGTENQGIVHVIGPEQGYTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLATQ
CLLQQKPQTAEIRIDGTLRPGVTAKDIILAIIAKIGVGGGTGYVLEYTGSAIRALTMEERMTICNMSIEGGARAGLIAPD
ETTFAWLKDRPHTPKGEAWDAAVEYWRTLPSDEGATYDLQVVLNADELAPMITYGTNPGMGIPVTGNVPAPSDLADDSQR
MALDKALNYMGLQPNQSLIGQKVDVVFLGSCTNSRISDLRAAAKVIEGKKVADGLRMLVVPGSQQVKRQAEAEGLDQIFR
AAGAEWREAGCSMCIAMNGDQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATAINGHIVDVREIL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=391, Percent_Identity=27.6214833759591, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=26.865671641791, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI41352693, Length=378, Percent_Identity=25.1322751322751, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=61.2765957446808, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=26.9230769230769, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI2367097, Length=115, Percent_Identity=35.6521739130435, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=475, Percent_Identity=28.8421052631579, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=29.1970802919708, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=30.0653594771242, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=382, Percent_Identity=26.7015706806283, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=55.6025369978858, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6320440, Length=460, Percent_Identity=28.9130434782609, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323335, Length=378, Percent_Identity=29.3650793650794, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=397, Percent_Identity=27.455919395466, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=26.5822784810127, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281365315, Length=479, Percent_Identity=25.4697286012526, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=479, Percent_Identity=25.4697286012526, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=390, Percent_Identity=26.4102564102564, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=26.5789473684211, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=25.3298153034301, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50305; Mature: 50174

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLFEKIWDAHVVQAADAESPATLYIDLHLVHEVTSPQAFTMLRERGLTVRRPAQTLA
CCHHHHHHHHHHHHEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCEECCHHHHH
TMDHSTPTTPRGADGIIPVTDAIARKQLDQLIKNCSDFGIPLYNLGTENQGIVHVIGPEQ
HCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCC
GYTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLATQCLLQQKPQTAEIRIDGTLR
CCCCCCCEEEECCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PGVTAKDIILAIIAKIGVGGGTGYVLEYTGSAIRALTMEERMTICNMSIEGGARAGLIAP
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHEEEEEEECCCCCCCCCCC
DETTFAWLKDRPHTPKGEAWDAAVEYWRTLPSDEGATYDLQVVLNADELAPMITYGTNPG
CCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCEEEEECCCCC
MGIPVTGNVPAPSDLADDSQRMALDKALNYMGLQPNQSLIGQKVDVVFLGSCTNSRISDL
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEECCCCHHHHHH
RAAAKVIEGKKVADGLRMLVVPGSQQVKRQAEAEGLDQIFRAAGAEWREAGCSMCIAMNG
HHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECC
DQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATAINGHIVDVREIL
CCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCEEEEHHHHC
>Mature Secondary Structure 
AKTLFEKIWDAHVVQAADAESPATLYIDLHLVHEVTSPQAFTMLRERGLTVRRPAQTLA
CHHHHHHHHHHHHEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCEECCHHHHH
TMDHSTPTTPRGADGIIPVTDAIARKQLDQLIKNCSDFGIPLYNLGTENQGIVHVIGPEQ
HCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCC
GYTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVGHVLATQCLLQQKPQTAEIRIDGTLR
CCCCCCCEEEECCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PGVTAKDIILAIIAKIGVGGGTGYVLEYTGSAIRALTMEERMTICNMSIEGGARAGLIAP
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHEEEEEEECCCCCCCCCCC
DETTFAWLKDRPHTPKGEAWDAAVEYWRTLPSDEGATYDLQVVLNADELAPMITYGTNPG
CCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCEEEEECCCCC
MGIPVTGNVPAPSDLADDSQRMALDKALNYMGLQPNQSLIGQKVDVVFLGSCTNSRISDL
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEECCCCHHHHHH
RAAAKVIEGKKVADGLRMLVVPGSQQVKRQAEAEGLDQIFRAAGAEWREAGCSMCIAMNG
HHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECC
DQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATAINGHIVDVREIL
CCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCEEEEHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA