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The map label for this gene is speE [H]

Identifier: 159900360

GI number: 159900360

Start: 4825494

End: 4827128

Strand: Direct

Name: speE [H]

Synonym: Haur_3843

Alternate gene names: 159900360

Gene position: 4825494-4827128 (Clockwise)

Preceding gene: 159900359

Following gene: 159900361

Centisome position: 76.03

GC content: 50.34

Gene sequence:

>1635_bases
ATGTTGTTAGTCTTTGGTGCGGGGATCTGTACGCTCGGCATCGAAATGACTGCTTCGCGGCTTTTGGCTCCTTACTTCGG
TACATCGCAGTTGATTTGGGCCAACGTCATTGGTTTAACTTTGCTCTATTTGACGATTGGCTATACCCTCGGCGGGCGTT
GGGCCGACCGTCGGCCTGAATTGGGCTTGCTTTGTACGATCATTTTGGTCGGGGCAATCACTGCTGCTGTGATCCCTTGG
ATCTCACGGCCAATTTTGAAGTGGTCGCTTGAGGCCTTTGCCACTCGTTCAGCGGGCTCGTTTATTGGCTCGTTGCTGGG
GGTTTTGGCCTTGTTCTCAGTGCCAATCACCTTGTTGGGCATGGTTAGCCCCTTCGCAGTACGCCTCGCGATTCGCGATG
CTGCCGATGCGGGCAAGGCGGCGGGCAACCTCTCGGCAATTTCGACGCTTGGCTCGATTCTCGGTACGTTCTTGCCGGTG
CTCTTCCTCATTCCCACGGTTGGCACAAATATGACAATTTATATTTTTGCCATGGGCTTGTTGGTGTTAATTTTGCCTGG
CTTCTGGTTGGAGCGGCGCAAGTTGCTGCCAGTCGCTTTGGTGGCCTTGATCGTAGTGGTGGCATTGGCATTTTGGCCGC
AAAGCGAGCCAATCAAGGTCGCCGATTGCCGTGGTTGTAGCCTGATTACTGAAGATGATTCGGCTTACAACTATATTCAG
ATTGTCGAACGGGGCAGCGATGCCAACAAAATGATCGGCTTGGTGCTCAACGAAGGCCAAGCCATCCACTCAATTTACTA
CCCAAAATTCGAGCAAACTAATAATGCTAGCGATTTGCTGACCAACGGGCCATGGGATTTTTATAATATTGCGCCTTACT
TCTACCCCAACCGTAGCATGGAAAGCGTCAACAGTATGGCCATGGTTGGCTCGGCGGCTGGCTCAGTGCCCAAACAATTT
TTAGCCATTTATGGCAAAGATTCAGTGATCGATGGAATTGAAATCGACCCACGAATTGTTGAACTAGGCCGTGAATACTT
CGCGATGCACGACGTGCAATATTATCAAGCACGCGGCGAAACTCCCCAGTTTCCTAATTTCAATACTTATGTTGCTGATG
GCCGAATTTTCTTCGCTGGCAACGACAATAAATATGATATTATCGGCATGGATGCCTACAAACAGCCCTATATTCCGTTT
CACCTCACCACCCGTGAGTTTTTTGAAACGGTACGCGACCATTTAGCTCCCAACGGAGTTGCTGTGGTTAATGCAGGCAA
GCCCAACACCGCTGCTGGCGGCGATTATCGCTTGGTCAATGTGTTGGCCAGCACCATGAGCAGCGTGTTTGCCCATGTCT
ATATGATCGATGTGCCGAATAATTTTAATACAATGTTGATTGGCATTCCTGGCGATGTTGGCGATGGCGTTGCTAACTTT
GAAGCCAATCTCGCAACCATTCAAGACCCGACTCTGCGCTATGTGATGGAGCAAGCACTAGCCAGCAATAGCAGCAAAAA
ACCAATTCGCCGTTGGGATGGCGTAGGCGAAAATCGGGTCTTTACCGACGACCTCGCCCCAGTTGAATCGGTGATCGACC
GAATTATTTTGGGGCAGGTTGATGAAGGCCAATAG

Upstream 100 bases:

>100_bases
ATTGATTTTTTAGTCATGCCGTGCCGTGGATGCAACGCCCAGTGGGTGGCTTCCACGGCTTTTTGTTGAAAGGATTCAAG
CATTGGCACGTCGTTACCTT

Downstream 100 bases:

>100_bases
TATGTCAACCAGCGATTCAGATTCACAAGAGATTAAATTGCGCCGCGCCAAGCGCCCCAAGGGCATTGTGCTGATGGTAC
TAGCCTTTTATGGAGCCGCC

Product: spermidine synthase-like protein

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MLLVFGAGICTLGIEMTASRLLAPYFGTSQLIWANVIGLTLLYLTIGYTLGGRWADRRPELGLLCTIILVGAITAAVIPW
ISRPILKWSLEAFATRSAGSFIGSLLGVLALFSVPITLLGMVSPFAVRLAIRDAADAGKAAGNLSAISTLGSILGTFLPV
LFLIPTVGTNMTIYIFAMGLLVLILPGFWLERRKLLPVALVALIVVVALAFWPQSEPIKVADCRGCSLITEDDSAYNYIQ
IVERGSDANKMIGLVLNEGQAIHSIYYPKFEQTNNASDLLTNGPWDFYNIAPYFYPNRSMESVNSMAMVGSAAGSVPKQF
LAIYGKDSVIDGIEIDPRIVELGREYFAMHDVQYYQARGETPQFPNFNTYVADGRIFFAGNDNKYDIIGMDAYKQPYIPF
HLTTREFFETVRDHLAPNGVAVVNAGKPNTAAGGDYRLVNVLASTMSSVFAHVYMIDVPNNFNTMLIGIPGDVGDGVANF
EANLATIQDPTLRYVMEQALASNSSKKPIRRWDGVGENRVFTDDLAPVESVIDRIILGQVDEGQ

Sequences:

>Translated_544_residues
MLLVFGAGICTLGIEMTASRLLAPYFGTSQLIWANVIGLTLLYLTIGYTLGGRWADRRPELGLLCTIILVGAITAAVIPW
ISRPILKWSLEAFATRSAGSFIGSLLGVLALFSVPITLLGMVSPFAVRLAIRDAADAGKAAGNLSAISTLGSILGTFLPV
LFLIPTVGTNMTIYIFAMGLLVLILPGFWLERRKLLPVALVALIVVVALAFWPQSEPIKVADCRGCSLITEDDSAYNYIQ
IVERGSDANKMIGLVLNEGQAIHSIYYPKFEQTNNASDLLTNGPWDFYNIAPYFYPNRSMESVNSMAMVGSAAGSVPKQF
LAIYGKDSVIDGIEIDPRIVELGREYFAMHDVQYYQARGETPQFPNFNTYVADGRIFFAGNDNKYDIIGMDAYKQPYIPF
HLTTREFFETVRDHLAPNGVAVVNAGKPNTAAGGDYRLVNVLASTMSSVFAHVYMIDVPNNFNTMLIGIPGDVGDGVANF
EANLATIQDPTLRYVMEQALASNSSKKPIRRWDGVGENRVFTDDLAPVESVIDRIILGQVDEGQ
>Mature_544_residues
MLLVFGAGICTLGIEMTASRLLAPYFGTSQLIWANVIGLTLLYLTIGYTLGGRWADRRPELGLLCTIILVGAITAAVIPW
ISRPILKWSLEAFATRSAGSFIGSLLGVLALFSVPITLLGMVSPFAVRLAIRDAADAGKAAGNLSAISTLGSILGTFLPV
LFLIPTVGTNMTIYIFAMGLLVLILPGFWLERRKLLPVALVALIVVVALAFWPQSEPIKVADCRGCSLITEDDSAYNYIQ
IVERGSDANKMIGLVLNEGQAIHSIYYPKFEQTNNASDLLTNGPWDFYNIAPYFYPNRSMESVNSMAMVGSAAGSVPKQF
LAIYGKDSVIDGIEIDPRIVELGREYFAMHDVQYYQARGETPQFPNFNTYVADGRIFFAGNDNKYDIIGMDAYKQPYIPF
HLTTREFFETVRDHLAPNGVAVVNAGKPNTAAGGDYRLVNVLASTMSSVFAHVYMIDVPNNFNTMLIGIPGDVGDGVANF
EANLATIQDPTLRYVMEQALASNSSKKPIRRWDGVGENRVFTDDLAPVESVIDRIILGQVDEGQ

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 59332; Mature: 59332

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVFGAGICTLGIEMTASRLLAPYFGTSQLIWANVIGLTLLYLTIGYTLGGRWADRRPE
CEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGLLCTIILVGAITAAVIPWISRPILKWSLEAFATRSAGSFIGSLLGVLALFSVPITLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVSPFAVRLAIRDAADAGKAAGNLSAISTLGSILGTFLPVLFLIPTVGTNMTIYIFAMGL
HHCHHHEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVLILPGFWLERRKLLPVALVALIVVVALAFWPQSEPIKVADCRGCSLITEDDSAYNYIQ
HHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCEEE
IVERGSDANKMIGLVLNEGQAIHSIYYPKFEQTNNASDLLTNGPWDFYNIAPYFYPNRSM
EEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEECCCEECCCCCH
ESVNSMAMVGSAAGSVPKQFLAIYGKDSVIDGIEIDPRIVELGREYFAMHDVQYYQARGE
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TPQFPNFNTYVADGRIFFAGNDNKYDIIGMDAYKQPYIPFHLTTREFFETVRDHLAPNGV
CCCCCCCCEEEECCEEEECCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCE
AVVNAGKPNTAAGGDYRLVNVLASTMSSVFAHVYMIDVPNNFNTMLIGIPGDVGDGVANF
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCC
EANLATIQDPTLRYVMEQALASNSSKKPIRRWDGVGENRVFTDDLAPVESVIDRIILGQV
CCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCC
DEGQ
CCCC
>Mature Secondary Structure
MLLVFGAGICTLGIEMTASRLLAPYFGTSQLIWANVIGLTLLYLTIGYTLGGRWADRRPE
CEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGLLCTIILVGAITAAVIPWISRPILKWSLEAFATRSAGSFIGSLLGVLALFSVPITLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVSPFAVRLAIRDAADAGKAAGNLSAISTLGSILGTFLPVLFLIPTVGTNMTIYIFAMGL
HHCHHHEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVLILPGFWLERRKLLPVALVALIVVVALAFWPQSEPIKVADCRGCSLITEDDSAYNYIQ
HHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCEEE
IVERGSDANKMIGLVLNEGQAIHSIYYPKFEQTNNASDLLTNGPWDFYNIAPYFYPNRSM
EEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCEECCCEECCCCCH
ESVNSMAMVGSAAGSVPKQFLAIYGKDSVIDGIEIDPRIVELGREYFAMHDVQYYQARGE
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TPQFPNFNTYVADGRIFFAGNDNKYDIIGMDAYKQPYIPFHLTTREFFETVRDHLAPNGV
CCCCCCCCEEEECCEEEECCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCE
AVVNAGKPNTAAGGDYRLVNVLASTMSSVFAHVYMIDVPNNFNTMLIGIPGDVGDGVANF
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCCCCCCCCCCC
EANLATIQDPTLRYVMEQALASNSSKKPIRRWDGVGENRVFTDDLAPVESVIDRIILGQV
CCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCC
DEGQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]