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The map label for this gene is gdhA [H]

Identifier: 159900011

GI number: 159900011

Start: 4400538

End: 4401788

Strand: Reverse

Name: gdhA [H]

Synonym: Haur_3494

Alternate gene names: 159900011

Gene position: 4401788-4400538 (Counterclockwise)

Preceding gene: 159900012

Following gene: 159900010

Centisome position: 69.36

GC content: 52.12

Gene sequence:

>1251_bases
ATGATTGAAACCACAATGGCAACCGCCCAGCGCCAATTTGATATTGCCGCTGAATTGCTGGCCTTAGAACCGGAAGTTCG
CGATATGCTCCGCGAGCCACGGCGTGAGTTGATCGTGCATTTTCCGGTCAAGCTGGATAATGGGCGGGTGCGAACTTTAA
CTGGCTATCGTGTCCATCATAATATTACGCGTGGCCCAGCCCTAGGTGGCTTGCGCTTACAATCGTCGGCTACGCTCGAT
GAAATGCAAGCTTTGGCGATGTGGATGACCTGGAGTTGCGCGATTGTGCAAATTCCCTATGGTGGAGCCAAGGGCGCGAT
CGTTTGCGATCATCGCGAATTGACTAGTGGCGAACTTGAGCGGATTATTCGGCGTTATGTGACCGAAATTACTCCATTGA
TCGGCGCTGAACGTGATGTGATCATGCCCGACCTTAACACCAATGAGCAAACCATGGCTTGGATTATGGATACCTATTCG
ATGCATCATGGCTACACAATGCCATCGGTTGCAACCGGTAAACCAGTTCAAGTTGGCGGTTCACAGGGCCATGGCCAAGG
AACTGCCCGTGGTTTGTGCTATACCGTGCGCCAAGCAGCCAGCAAAATTGGCCTCGATTTGGCGGGAGCACGGGTGGTGA
TGCAAGGTTCCGGCTCGACTGGGCGTTTGGTGCTGCAATTCTTGGCGGAAATGGGCTGTCGGATTATCGCTGCCAGCGAT
GATACTACCGGCATTGTCGCTGAATCCGAGGCTGGAATCGATGTTGCTGCTTTGATTGAACATCGAGTTCAGACCGATGG
CGTAGCTAATTTGGCTGGCACAAAGCCGATTAGCCATAACGAATTGCTCGAATTACCATGCGATATTTTGGTGCTCGCAG
CAGGCCAAAACGATATTAGCGGAGCCAATGCTGGCCGAATTCAAGCTAAAATTGTGGTCGAATTAGCCAATGGTCCAATC
ACGCCAACCGGCGATTCGATTCTCGCCAACAAACAAATTATGGTTGTGCCCGATATCTTGGCCAATGCAGGCGGGATTGT
GGTTTCCTACTTCGAATGGGTCCAGGGCTTGCAAGAGTTTTTTTGGACCGAACGCGAAGTTCATGGCGAATTAGCCACGA
CCATGGAACGGGCCTTTAACGAGGTCTTTACCGTCGCCCAAGAACGTTTTGTGCCATTACGCACAGCGGCCTATCTGCTA
GCCGTTGATCGCGTCGTCCGTGCGATGGCGATGCGTGGGATTTATCCGTAG

Upstream 100 bases:

>100_bases
GGGCGCTCGCACGAACCATGCGGAGTGTGTTAAGATACATGCACTTAACGATTATGCCTAAAGCAGCGTTGCTTTACACT
CACTGCCGAGGAGTTTTACG

Downstream 100 bases:

>100_bases
TAGCTGGGGATCGGGGGATCGGTTGTTGGGGATCGGGATAGTTTCGTTCTCTCACCCCCCGCGGCGGGCGAGGGGAGTTG
TCGGCTCATGATAGGCTGCT

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MIETTMATAQRQFDIAAELLALEPEVRDMLREPRRELIVHFPVKLDNGRVRTLTGYRVHHNITRGPALGGLRLQSSATLD
EMQALAMWMTWSCAIVQIPYGGAKGAIVCDHRELTSGELERIIRRYVTEITPLIGAERDVIMPDLNTNEQTMAWIMDTYS
MHHGYTMPSVATGKPVQVGGSQGHGQGTARGLCYTVRQAASKIGLDLAGARVVMQGSGSTGRLVLQFLAEMGCRIIAASD
DTTGIVAESEAGIDVAALIEHRVQTDGVANLAGTKPISHNELLELPCDILVLAAGQNDISGANAGRIQAKIVVELANGPI
TPTGDSILANKQIMVVPDILANAGGIVVSYFEWVQGLQEFFWTEREVHGELATTMERAFNEVFTVAQERFVPLRTAAYLL
AVDRVVRAMAMRGIYP

Sequences:

>Translated_416_residues
MIETTMATAQRQFDIAAELLALEPEVRDMLREPRRELIVHFPVKLDNGRVRTLTGYRVHHNITRGPALGGLRLQSSATLD
EMQALAMWMTWSCAIVQIPYGGAKGAIVCDHRELTSGELERIIRRYVTEITPLIGAERDVIMPDLNTNEQTMAWIMDTYS
MHHGYTMPSVATGKPVQVGGSQGHGQGTARGLCYTVRQAASKIGLDLAGARVVMQGSGSTGRLVLQFLAEMGCRIIAASD
DTTGIVAESEAGIDVAALIEHRVQTDGVANLAGTKPISHNELLELPCDILVLAAGQNDISGANAGRIQAKIVVELANGPI
TPTGDSILANKQIMVVPDILANAGGIVVSYFEWVQGLQEFFWTEREVHGELATTMERAFNEVFTVAQERFVPLRTAAYLL
AVDRVVRAMAMRGIYP
>Mature_416_residues
MIETTMATAQRQFDIAAELLALEPEVRDMLREPRRELIVHFPVKLDNGRVRTLTGYRVHHNITRGPALGGLRLQSSATLD
EMQALAMWMTWSCAIVQIPYGGAKGAIVCDHRELTSGELERIIRRYVTEITPLIGAERDVIMPDLNTNEQTMAWIMDTYS
MHHGYTMPSVATGKPVQVGGSQGHGQGTARGLCYTVRQAASKIGLDLAGARVVMQGSGSTGRLVLQFLAEMGCRIIAASD
DTTGIVAESEAGIDVAALIEHRVQTDGVANLAGTKPISHNELLELPCDILVLAAGQNDISGANAGRIQAKIVVELANGPI
TPTGDSILANKQIMVVPDILANAGGIVVSYFEWVQGLQEFFWTEREVHGELATTMERAFNEVFTVAQERFVPLRTAAYLL
AVDRVVRAMAMRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=443, Percent_Identity=31.8284424379233, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4885281, Length=443, Percent_Identity=31.8284424379233, Blast_Score=231, Evalue=7e-61,
Organism=Escherichia coli, GI1788059, Length=375, Percent_Identity=29.3333333333333, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17544676, Length=337, Percent_Identity=35.9050445103858, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6319256, Length=388, Percent_Identity=28.6082474226804, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324951, Length=408, Percent_Identity=27.4509803921569, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45553475, Length=375, Percent_Identity=34.1333333333333, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45549226, Length=374, Percent_Identity=33.9572192513369, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24649283, Length=337, Percent_Identity=36.2017804154303, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24649460, Length=313, Percent_Identity=33.8658146964856, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45247; Mature: 45247

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETTMATAQRQFDIAAELLALEPEVRDMLREPRRELIVHFPVKLDNGRVRTLTGYRVHH
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEEEEEECCCEEEEEEEEEEEC
NITRGPALGGLRLQSSATLDEMQALAMWMTWSCAIVQIPYGGAKGAIVCDHRELTSGELE
CCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECCCCCCHHHHH
RIIRRYVTEITPLIGAERDVIMPDLNTNEQTMAWIMDTYSMHHGYTMPSVATGKPVQVGG
HHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
SQGHGQGTARGLCYTVRQAASKIGLDLAGARVVMQGSGSTGRLVLQFLAEMGCRIIAASD
CCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHCCCEEEEECC
DTTGIVAESEAGIDVAALIEHRVQTDGVANLAGTKPISHNELLELPCDILVLAAGQNDIS
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCC
GANAGRIQAKIVVELANGPITPTGDSILANKQIMVVPDILANAGGIVVSYFEWVQGLQEF
CCCCCEEEEEEEEEECCCCCCCCCCCCEECCEEEEECHHHCCCCCHHHHHHHHHHHHHHH
FWTEREVHGELATTMERAFNEVFTVAQERFVPLRTAAYLLAVDRVVRAMAMRGIYP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIETTMATAQRQFDIAAELLALEPEVRDMLREPRRELIVHFPVKLDNGRVRTLTGYRVHH
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEEEEEECCCEEEEEEEEEEEC
NITRGPALGGLRLQSSATLDEMQALAMWMTWSCAIVQIPYGGAKGAIVCDHRELTSGELE
CCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCEEECCCCCCHHHHH
RIIRRYVTEITPLIGAERDVIMPDLNTNEQTMAWIMDTYSMHHGYTMPSVATGKPVQVGG
HHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
SQGHGQGTARGLCYTVRQAASKIGLDLAGARVVMQGSGSTGRLVLQFLAEMGCRIIAASD
CCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHHHCCCEEEEECC
DTTGIVAESEAGIDVAALIEHRVQTDGVANLAGTKPISHNELLELPCDILVLAAGQNDIS
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCC
GANAGRIQAKIVVELANGPITPTGDSILANKQIMVVPDILANAGGIVVSYFEWVQGLQEF
CCCCCEEEEEEEEEECCCCCCCCCCCCEECCEEEEECHHHCCCCCHHHHHHHHHHHHHHH
FWTEREVHGELATTMERAFNEVFTVAQERFVPLRTAAYLLAVDRVVRAMAMRGIYP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]