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The map label for this gene is bfmBB [H]

Identifier: 159899112

GI number: 159899112

Start: 3327569

End: 3328888

Strand: Direct

Name: bfmBB [H]

Synonym: Haur_2593

Alternate gene names: 159899112

Gene position: 3327569-3328888 (Clockwise)

Preceding gene: 159899111

Following gene: 159899113

Centisome position: 52.43

GC content: 54.32

Gene sequence:

>1320_bases
ATGTCCGTCGAATTTAAGATGCCAAAGCTTGGTGAGAGCGTCACCGAGGGCACAGTTGGGCGCTGGCTCAAGCAACCAGG
CGAGAGCCTTGAACTCTATGAGCCAATGCTCGAAGTTACTACCGACAAAGTGGATACCGAAATTCCTTCGCCAGTCAATG
GGCGATTGCTCGAAATTCGGGTCAACGAAGGCGATACCGTGCCAGTTGGTACGATCATTGCGGTCTTGGAAGATAGTAGT
AGCGCTACGGTTGCCGCCGTTGGCGCACCAGCCGCGCCCGCCGTCGCCAGCCAAGCAAGCAGCAATAACGGCGGTGGCAA
TAGCTTTATGTCACCAGTCGTGGCTCGCTTGGTCAGCCAGCATAACCTCGATATTAAGCAAATTGCTGGCACTGGCAAAG
ATGGCCGCGTGACCAAGCAAGATGTTGAGCGCTTTATGGCTCAGGGTGATGCTGCCAAACCAGCACCGATGCCAATGCCA
ACGCCAACCGCGCCAACCCCAGCGCCGATGCCCGTCATCAAACCAAGCTCAACGCCAGCACCAATGCCGACGATTACTGG
CAGTTTGCTGGCCAAACCAGTGGCTTATACTCCGCCAGCCACGCCAGCCGCGCCGAGCATCGAGCCATTTGTTGGTGATG
AATTACAACCGTTGAGTGGTATGCGCCGCGCAATCGCTGAGCATATGGTGCGCTCAAAAGCAACCTCGCCACACGTTACC
ACAGTGATGGAAGTTGATCTCAGCACAATTATCGCGCATCGTGACCAACATAAGGCCGATTTCGAGCGGCAAGGGGTTAA
ATTAACCTTTACGCCCTATTTTGTGCAGGCAACTGTAGCAGGCTTACGAGCAGTGCCAATTGTCAATGCCACCTATACCG
ATGAAGGCATTTTGCTGCATAAATCGGTTAACGTTGGCATGGCCGTGGCCATTCCCGATGGCCTGATCGTGCCAGTGCTG
CATAACGCCGATGAAAAGAGCTTGCTGGGGCTTTCGCGCACAGTCAACGATTTGGCCGAACGCGCCCGCACGCGCCGCCT
AACCGCCGATGAAGTGAAAGACGGCACCTTTACAATTACAAATCACGGCGTAAGTGGCTCGCTATTTGCCATGCCAATCA
TCAACCAGCCTCAATCGGGCATTTTGGGGATTGGTGCAATCCAAAAGCGGCCTGTGGTCGTGACGATCAACGGAGCCGAT
GCAATCGCGATTCGGCCAATGTGCTACCTCAGTTTTACGTTTGACCATCGCTTGATCGACGGCGCAACCGCCGATAAATT
CTTGGCAACCGTCAAGCAACAACTTGAGCAATTTGCCTAG

Upstream 100 bases:

>100_bases
TAGTGGCGATTGGCTGTAGGCGATTGGCTATTGGATAGTGAAGATCCAACTTGGCTTTAGCTTATAGCCCATCAGTATCG
ATACCATAGAAAGGACTGGG

Downstream 100 bases:

>100_bases
ATTAACCCGAAACCCCCTTGCTGATTCGGATCAGCAAGGGGGTTTTGTTTAATCCAAAACCCTTCTCACAATTTCTGCAT
CTGCTATATACTTATCCTAA

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSVEFKMPKLGESVTEGTVGRWLKQPGESLELYEPMLEVTTDKVDTEIPSPVNGRLLEIRVNEGDTVPVGTIIAVLEDSS
SATVAAVGAPAAPAVASQASSNNGGGNSFMSPVVARLVSQHNLDIKQIAGTGKDGRVTKQDVERFMAQGDAAKPAPMPMP
TPTAPTPAPMPVIKPSSTPAPMPTITGSLLAKPVAYTPPATPAAPSIEPFVGDELQPLSGMRRAIAEHMVRSKATSPHVT
TVMEVDLSTIIAHRDQHKADFERQGVKLTFTPYFVQATVAGLRAVPIVNATYTDEGILLHKSVNVGMAVAIPDGLIVPVL
HNADEKSLLGLSRTVNDLAERARTRRLTADEVKDGTFTITNHGVSGSLFAMPIINQPQSGILGIGAIQKRPVVVTINGAD
AIAIRPMCYLSFTFDHRLIDGATADKFLATVKQQLEQFA

Sequences:

>Translated_439_residues
MSVEFKMPKLGESVTEGTVGRWLKQPGESLELYEPMLEVTTDKVDTEIPSPVNGRLLEIRVNEGDTVPVGTIIAVLEDSS
SATVAAVGAPAAPAVASQASSNNGGGNSFMSPVVARLVSQHNLDIKQIAGTGKDGRVTKQDVERFMAQGDAAKPAPMPMP
TPTAPTPAPMPVIKPSSTPAPMPTITGSLLAKPVAYTPPATPAAPSIEPFVGDELQPLSGMRRAIAEHMVRSKATSPHVT
TVMEVDLSTIIAHRDQHKADFERQGVKLTFTPYFVQATVAGLRAVPIVNATYTDEGILLHKSVNVGMAVAIPDGLIVPVL
HNADEKSLLGLSRTVNDLAERARTRRLTADEVKDGTFTITNHGVSGSLFAMPIINQPQSGILGIGAIQKRPVVVTINGAD
AIAIRPMCYLSFTFDHRLIDGATADKFLATVKQQLEQFA
>Mature_438_residues
SVEFKMPKLGESVTEGTVGRWLKQPGESLELYEPMLEVTTDKVDTEIPSPVNGRLLEIRVNEGDTVPVGTIIAVLEDSSS
ATVAAVGAPAAPAVASQASSNNGGGNSFMSPVVARLVSQHNLDIKQIAGTGKDGRVTKQDVERFMAQGDAAKPAPMPMPT
PTAPTPAPMPVIKPSSTPAPMPTITGSLLAKPVAYTPPATPAAPSIEPFVGDELQPLSGMRRAIAEHMVRSKATSPHVTT
VMEVDLSTIIAHRDQHKADFERQGVKLTFTPYFVQATVAGLRAVPIVNATYTDEGILLHKSVNVGMAVAIPDGLIVPVLH
NADEKSLLGLSRTVNDLAERARTRRLTADEVKDGTFTITNHGVSGSLFAMPIINQPQSGILGIGAIQKRPVVVTINGADA
IAIRPMCYLSFTFDHRLIDGATADKFLATVKQQLEQFA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=450, Percent_Identity=27.7777777777778, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI19923748, Length=244, Percent_Identity=38.5245901639344, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI31711992, Length=466, Percent_Identity=28.1115879828326, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI203098816, Length=472, Percent_Identity=25, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI203098753, Length=472, Percent_Identity=25, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=29.0123456790123, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786946, Length=439, Percent_Identity=34.624145785877, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1786305, Length=454, Percent_Identity=26.6519823788546, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=447, Percent_Identity=26.3982102908277, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=474, Percent_Identity=27.2151898734177, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=341, Percent_Identity=29.3255131964809, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=437, Percent_Identity=31.350114416476, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI18859875, Length=455, Percent_Identity=27.9120879120879, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=37.5565610859729, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=30.1801801801802, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=30.1801801801802, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46538; Mature: 46407

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEFKMPKLGESVTEGTVGRWLKQPGESLELYEPMLEVTTDKVDTEIPSPVNGRLLEIR
CCCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
VNEGDTVPVGTIIAVLEDSSSATVAAVGAPAAPAVASQASSNNGGGNSFMSPVVARLVSQ
ECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
HNLDIKQIAGTGKDGRVTKQDVERFMAQGDAAKPAPMPMPTPTAPTPAPMPVIKPSSTPA
CCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCC
PMPTITGSLLAKPVAYTPPATPAAPSIEPFVGDELQPLSGMRRAIAEHMVRSKATSPHVT
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
TVMEVDLSTIIAHRDQHKADFERQGVKLTFTPYFVQATVAGLRAVPIVNATYTDEGILLH
EEEEEHHHHHHHHCCCHHHHHHHCCEEEEECHHHHHHHHHHHEEEEEEEEEECCCCEEEE
KSVNVGMAVAIPDGLIVPVLHNADEKSLLGLSRTVNDLAERARTRRLTADEVKDGTFTIT
EECCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEE
NHGVSGSLFAMPIINQPQSGILGIGAIQKRPVVVTINGADAIAIRPMCYLSFTFDHRLID
ECCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEEECEEEEEEEEECCEEEC
GATADKFLATVKQQLEQFA
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVEFKMPKLGESVTEGTVGRWLKQPGESLELYEPMLEVTTDKVDTEIPSPVNGRLLEIR
CCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
VNEGDTVPVGTIIAVLEDSSSATVAAVGAPAAPAVASQASSNNGGGNSFMSPVVARLVSQ
ECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
HNLDIKQIAGTGKDGRVTKQDVERFMAQGDAAKPAPMPMPTPTAPTPAPMPVIKPSSTPA
CCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCC
PMPTITGSLLAKPVAYTPPATPAAPSIEPFVGDELQPLSGMRRAIAEHMVRSKATSPHVT
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
TVMEVDLSTIIAHRDQHKADFERQGVKLTFTPYFVQATVAGLRAVPIVNATYTDEGILLH
EEEEEHHHHHHHHCCCHHHHHHHCCEEEEECHHHHHHHHHHHEEEEEEEEEECCCCEEEE
KSVNVGMAVAIPDGLIVPVLHNADEKSLLGLSRTVNDLAERARTRRLTADEVKDGTFTIT
EECCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEE
NHGVSGSLFAMPIINQPQSGILGIGAIQKRPVVVTINGADAIAIRPMCYLSFTFDHRLID
ECCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEEECEEEEEEEEECCEEEC
GATADKFLATVKQQLEQFA
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]