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The map label for this gene is ysgA [H]

Identifier: 159899036

GI number: 159899036

Start: 3214513

End: 3215313

Strand: Reverse

Name: ysgA [H]

Synonym: Haur_2517

Alternate gene names: 159899036

Gene position: 3215313-3214513 (Counterclockwise)

Preceding gene: 159899037

Following gene: 159899030

Centisome position: 50.66

GC content: 51.69

Gene sequence:

>801_bases
GTGGAGCTAGAGCAAACCGTTTTACCCGATATTGTCAAAGAAATTCGTCAATTGCGCTCGCGCGATGCCCGCGACCGAAC
GGGAACGTACTATATTGAAGGGGCGCGAATCGTTGCCCAAGCAATTAATGCTGGTTTGCCGCTGGAAATTGGGGCGATCT
CGCTGGAATTGCTCTCACGCACTGAGCATTCTGCTGGCACTGTCAGTGCTTTGCGCAAAGCTGCCAAACAAATGGTTGAA
CTTTCGGCGAGTGCCTTTGCAGGCATTTCATTTAAAGAAAATCTGCAAGGCATCGGGGCCGTGGTCAAATTGCAGCAAGA
GCCGCTGAGCAATGTTCAATTGACTGATCGGCCTTGGGTGGCCTTGAATGGAGTTGGTAACCCTGGCAACTTGGGCGCGA
TTATGCGCACCTGTGATGCAGTTGGTTGCGAAGGCCTAATTTTGATTGGCGACACGACCGATCCTTATCATCCGGCAGCG
ATTCGCGCCAGTATGGGGTCATTGTTTGCTTTGCGTATGGTGCGCACAACCTTCGATGAATTTGCCGCGTGGAAACAAAC
CAATGGCTATCATGTGATTGGCACGACCCCCGATGTTGAGCAGGAATACCAAACCATCAGCTACCCAAGCCCCGCGATTT
TGCTGATGGGCAGTGAACGCTTGGGCTTATCGGTTGGTGAGCAAAGCGTCTGTGATACCTTGGTGCGCATTCCCATGGCT
GGCACCTGCGATTCGCTGAATTTAGGGGTGGCGACTAGCATTGTGTTGTATGAAATGTTTCGTCAACAACGCCTAGTCTA
A

Upstream 100 bases:

>100_bases
CCCGCCAACAAGCCCACAACGCCCGGCCCGACCGCCTGCCAAACGCCCAGAACGCTCGCATCAAGCGCGTAAACCTGTGT
CGCAAGGGCAAAAACCAACC

Downstream 100 bases:

>100_bases
TCGAACCTTGCTTAAGTTTGTAATCCGACTGGCTGAATTAAGCTAGTCGGATTTTTTAGGGCTAAAACTACAAACGGCCC
AGTTGGGCCGCTTGTAGGAG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MELEQTVLPDIVKEIRQLRSRDARDRTGTYYIEGARIVAQAINAGLPLEIGAISLELLSRTEHSAGTVSALRKAAKQMVE
LSASAFAGISFKENLQGIGAVVKLQQEPLSNVQLTDRPWVALNGVGNPGNLGAIMRTCDAVGCEGLILIGDTTDPYHPAA
IRASMGSLFALRMVRTTFDEFAAWKQTNGYHVIGTTPDVEQEYQTISYPSPAILLMGSERLGLSVGEQSVCDTLVRIPMA
GTCDSLNLGVATSIVLYEMFRQQRLV

Sequences:

>Translated_266_residues
MELEQTVLPDIVKEIRQLRSRDARDRTGTYYIEGARIVAQAINAGLPLEIGAISLELLSRTEHSAGTVSALRKAAKQMVE
LSASAFAGISFKENLQGIGAVVKLQQEPLSNVQLTDRPWVALNGVGNPGNLGAIMRTCDAVGCEGLILIGDTTDPYHPAA
IRASMGSLFALRMVRTTFDEFAAWKQTNGYHVIGTTPDVEQEYQTISYPSPAILLMGSERLGLSVGEQSVCDTLVRIPMA
GTCDSLNLGVATSIVLYEMFRQQRLV
>Mature_266_residues
MELEQTVLPDIVKEIRQLRSRDARDRTGTYYIEGARIVAQAINAGLPLEIGAISLELLSRTEHSAGTVSALRKAAKQMVE
LSASAFAGISFKENLQGIGAVVKLQQEPLSNVQLTDRPWVALNGVGNPGNLGAIMRTCDAVGCEGLILIGDTTDPYHPAA
IRASMGSLFALRMVRTTFDEFAAWKQTNGYHVIGTTPDVEQEYQTISYPSPAILLMGSERLGLSVGEQSVCDTLVRIPMA
GTCDSLNLGVATSIVLYEMFRQQRLV

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=175, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790083, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788935, Length=150, Percent_Identity=29.3333333333333, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28798; Mature: 28798

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEQTVLPDIVKEIRQLRSRDARDRTGTYYIEGARIVAQAINAGLPLEIGAISLELLSR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCCCEEEHHHHHHHHHH
TEHSAGTVSALRKAAKQMVELSASAFAGISFKENLQGIGAVVKLQQEPLSNVQLTDRPWV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCEECCCCEE
ALNGVGNPGNLGAIMRTCDAVGCEGLILIGDTTDPYHPAAIRASMGSLFALRMVRTTFDE
EEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAAWKQTNGYHVIGTTPDVEQEYQTISYPSPAILLMGSERLGLSVGEQSVCDTLVRIPMA
HHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCC
GTCDSLNLGVATSIVLYEMFRQQRLV
CCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELEQTVLPDIVKEIRQLRSRDARDRTGTYYIEGARIVAQAINAGLPLEIGAISLELLSR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCCCEEEHHHHHHHHHH
TEHSAGTVSALRKAAKQMVELSASAFAGISFKENLQGIGAVVKLQQEPLSNVQLTDRPWV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCEECCCCEE
ALNGVGNPGNLGAIMRTCDAVGCEGLILIGDTTDPYHPAAIRASMGSLFALRMVRTTFDE
EEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAAWKQTNGYHVIGTTPDVEQEYQTISYPSPAILLMGSERLGLSVGEQSVCDTLVRIPMA
HHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCC
GTCDSLNLGVATSIVLYEMFRQQRLV
CCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]