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The map label for this gene is murE [H]

Identifier: 159896617

GI number: 159896617

Start: 109251

End: 110795

Strand: Direct

Name: murE [H]

Synonym: Haur_0084

Alternate gene names: 159896617

Gene position: 109251-110795 (Clockwise)

Preceding gene: 159896616

Following gene: 159896621

Centisome position: 1.72

GC content: 53.14

Gene sequence:

>1545_bases
ATGAAATCGTTCGCTGAACTTTTGCTGCATGTTACCCATCAAGCTTCTGGCGATCACCACGTTGCAATTAGCAATGTAGC
TTACGATTCGCGCAAAGTTACACCTGGAGCGCTGTTTGTGGCGATCAAAGGGGTACATGTTGATGGTCATCGTTATATTG
CAACGGCCTTGGAGCAAGGCGCGGTGGCAGTGGTCTATGATGATCCTCATCAAGCGCCGCCTGCCACTATTCCCTCGGCT
TTGGTTGCCGATGCCAAGGTTGCGCTTTCGCCGATTGCCGCCGCCTTTTACGATTACCCCGCCCAGCAATTAGAAGTGGT
AGGCATTACCGGCTCAAAGGGCAAAACCACCACCACCTTTTTAACCGCAGCCGCCCTAGAAGCTAGCGGCGAGTTGGTGG
GCTTTATGAGCACTGTCGATTTTAAAATTGGCCAGCGCCAATGGGCCAACAAAACCCGCCAATCGACCCCCGAAGCCTTG
GAAATTCAGGCCATGTTGCGCGAGATGGTCGAGGCTGGTTGTCGCTATGCTGTGGTCGAAAGCACGTCGCATGGCCTCTC
GCCCCGTTGGAATCGGCTGGGCGATTGTGCCTATGATGTGGCCTTGATTACCAACGTTACCCACGAACACCTTGATTATC
ATGGCACCGTTGAGCAATATCGTGCTGATAAAGCTCAATTATTTCAGTTGCTCGCCACTAGCCCCAGCCAAAAATCGATT
CTTGGCCAACCAACGCCAGTCGAAAAAGTCGCAATCGTTAATGCCGATGATCCCCATGCGGCCCAATTTCGGGCAGCGGC
TGGCGAGCAAGTGCGGCATTTGAGCTATGCAATTCATGCTGAGGCTGATCTTCGGGCTACCGAAATTGTCTCAACTAGCA
AGGGCTTGCGGTTTACCGCAATTACGCCGCAAGGTAACTTTGCGCTCAATTTGCAATTGGTTGGCACATTTTATGTTTAC
AACGTGTTAGCAGCGCTCAGTGTCTGTTTGGCGCGAGGCGTTGATCTGGCGGCGGCGATTCAGCGGCTTGAGGCAATTCC
AGGCATCAACGGTCGCATGGAGCAAGTTGAGCTTGGCCAGCCATTTAGTGTGCTAGTCGATTATGCCCATAACCCCGATT
CGTTTGAGCAAGTGATGAGTATGCTGCGGCCATTGACCCAAGGCCGTTTGATTGCGGTATTTGGTTCAGCAGGCGAGCGC
GATCTTGCCAAGCGCCCGATTCAAGGCCAAATTGCGGCCAAATGGTGCGATCTGCTCTATCTGACCGATGAAGATCCCCG
AGCCGAGGATTCGCTACAGATTTTGCAGGAAATTGCTGCTGGCGCAAAGGCTGAGGGCAAAATTATCGATCAAAATTGCT
GGCTGATTCCCGATCGGCGAACGGCCATCGAGCAAGCGCTGGCAACCGCCCAAGCTGGCGATGTGGTGCTGTTGCTAGGC
AAAGGCCACGAAGGCAACATCATTTATGCCAGCGGCGATATCGAATGGAACGAGCATGCCGAGGCTGAACGCGCATTGCA
AGGCTTGGGCTACATCAAACGATAA

Upstream 100 bases:

>100_bases
GGGCGGTCAACCAGTCCGCTATCTGCCAGCCTATGATCGCGTTTTCTTGCGTCCGGCGTATTGGCTTTGGCAGCGTCGCC
GCTCTGAATAGGAACAATCA

Downstream 100 bases:

>100_bases
ATTCGTTTGAGCCGATCGAGTTCGGTGTGGGTCACTTCTGTCAGGAACGGCTTAAGATCCTGAACTGGTAAAAGGGCGTG
ATAGCCACGGCGTAAAAAGC

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKSFAELLLHVTHQASGDHHVAISNVAYDSRKVTPGALFVAIKGVHVDGHRYIATALEQGAVAVVYDDPHQAPPATIPSA
LVADAKVALSPIAAAFYDYPAQQLEVVGITGSKGKTTTTFLTAAALEASGELVGFMSTVDFKIGQRQWANKTRQSTPEAL
EIQAMLREMVEAGCRYAVVESTSHGLSPRWNRLGDCAYDVALITNVTHEHLDYHGTVEQYRADKAQLFQLLATSPSQKSI
LGQPTPVEKVAIVNADDPHAAQFRAAAGEQVRHLSYAIHAEADLRATEIVSTSKGLRFTAITPQGNFALNLQLVGTFYVY
NVLAALSVCLARGVDLAAAIQRLEAIPGINGRMEQVELGQPFSVLVDYAHNPDSFEQVMSMLRPLTQGRLIAVFGSAGER
DLAKRPIQGQIAAKWCDLLYLTDEDPRAEDSLQILQEIAAGAKAEGKIIDQNCWLIPDRRTAIEQALATAQAGDVVLLLG
KGHEGNIIYASGDIEWNEHAEAERALQGLGYIKR

Sequences:

>Translated_514_residues
MKSFAELLLHVTHQASGDHHVAISNVAYDSRKVTPGALFVAIKGVHVDGHRYIATALEQGAVAVVYDDPHQAPPATIPSA
LVADAKVALSPIAAAFYDYPAQQLEVVGITGSKGKTTTTFLTAAALEASGELVGFMSTVDFKIGQRQWANKTRQSTPEAL
EIQAMLREMVEAGCRYAVVESTSHGLSPRWNRLGDCAYDVALITNVTHEHLDYHGTVEQYRADKAQLFQLLATSPSQKSI
LGQPTPVEKVAIVNADDPHAAQFRAAAGEQVRHLSYAIHAEADLRATEIVSTSKGLRFTAITPQGNFALNLQLVGTFYVY
NVLAALSVCLARGVDLAAAIQRLEAIPGINGRMEQVELGQPFSVLVDYAHNPDSFEQVMSMLRPLTQGRLIAVFGSAGER
DLAKRPIQGQIAAKWCDLLYLTDEDPRAEDSLQILQEIAAGAKAEGKIIDQNCWLIPDRRTAIEQALATAQAGDVVLLLG
KGHEGNIIYASGDIEWNEHAEAERALQGLGYIKR
>Mature_514_residues
MKSFAELLLHVTHQASGDHHVAISNVAYDSRKVTPGALFVAIKGVHVDGHRYIATALEQGAVAVVYDDPHQAPPATIPSA
LVADAKVALSPIAAAFYDYPAQQLEVVGITGSKGKTTTTFLTAAALEASGELVGFMSTVDFKIGQRQWANKTRQSTPEAL
EIQAMLREMVEAGCRYAVVESTSHGLSPRWNRLGDCAYDVALITNVTHEHLDYHGTVEQYRADKAQLFQLLATSPSQKSI
LGQPTPVEKVAIVNADDPHAAQFRAAAGEQVRHLSYAIHAEADLRATEIVSTSKGLRFTAITPQGNFALNLQLVGTFYVY
NVLAALSVCLARGVDLAAAIQRLEAIPGINGRMEQVELGQPFSVLVDYAHNPDSFEQVMSMLRPLTQGRLIAVFGSAGER
DLAKRPIQGQIAAKWCDLLYLTDEDPRAEDSLQILQEIAAGAKAEGKIIDQNCWLIPDRRTAIEQALATAQAGDVVLLLG
KGHEGNIIYASGDIEWNEHAEAERALQGLGYIKR

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=496, Percent_Identity=31.8548387096774, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786274, Length=383, Percent_Identity=27.6762402088773, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 55588; Mature: 55588

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFAELLLHVTHQASGDHHVAISNVAYDSRKVTPGALFVAIKGVHVDGHRYIATALEQG
CCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHHHHCC
AVAVVYDDPHQAPPATIPSALVADAKVALSPIAAAFYDYPAQQLEVVGITGSKGKTTTTF
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHE
LTAAALEASGELVGFMSTVDFKIGQRQWANKTRQSTPEALEIQAMLREMVEAGCRYAVVE
EEHHHHHCCCCEEEEEEHHCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
STSHGLSPRWNRLGDCAYDVALITNVTHEHLDYHGTVEQYRADKAQLFQLLATSPSQKSI
CCCCCCCCCHHHHCCHHHHHHHHHHCHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCC
LGQPTPVEKVAIVNADDPHAAQFRAAAGEQVRHLSYAIHAEADLRATEIVSTSKGLRFTA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCEEEE
ITPQGNFALNLQLVGTFYVYNVLAALSVCLARGVDLAAAIQRLEAIPGINGRMEQVELGQ
ECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCC
PFSVLVDYAHNPDSFEQVMSMLRPLTQGRLIAVFGSAGERDLAKRPIQGQIAAKWCDLLY
CHHHHEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCHHHEEEEEEEE
LTDEDPRAEDSLQILQEIAAGAKAEGKIIDQNCWLIPDRRTAIEQALATAQAGDVVLLLG
EECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCCHHHHHHHHHHHCCCCCEEEEEE
KGHEGNIIYASGDIEWNEHAEAERALQGLGYIKR
CCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSFAELLLHVTHQASGDHHVAISNVAYDSRKVTPGALFVAIKGVHVDGHRYIATALEQG
CCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHHHHCC
AVAVVYDDPHQAPPATIPSALVADAKVALSPIAAAFYDYPAQQLEVVGITGSKGKTTTTF
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHE
LTAAALEASGELVGFMSTVDFKIGQRQWANKTRQSTPEALEIQAMLREMVEAGCRYAVVE
EEHHHHHCCCCEEEEEEHHCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
STSHGLSPRWNRLGDCAYDVALITNVTHEHLDYHGTVEQYRADKAQLFQLLATSPSQKSI
CCCCCCCCCHHHHCCHHHHHHHHHHCHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCC
LGQPTPVEKVAIVNADDPHAAQFRAAAGEQVRHLSYAIHAEADLRATEIVSTSKGLRFTA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCEEEE
ITPQGNFALNLQLVGTFYVYNVLAALSVCLARGVDLAAAIQRLEAIPGINGRMEQVELGQ
ECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCC
PFSVLVDYAHNPDSFEQVMSMLRPLTQGRLIAVFGSAGERDLAKRPIQGQIAAKWCDLLY
CHHHHEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCHHHEEEEEEEE
LTDEDPRAEDSLQILQEIAAGAKAEGKIIDQNCWLIPDRRTAIEQALATAQAGDVVLLLG
EECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCEECCCHHHHHHHHHHHCCCCCEEEEEE
KGHEGNIIYASGDIEWNEHAEAERALQGLGYIKR
CCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA