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The map label for this gene is rffG [C]

Identifier: 159041441

GI number: 159041441

Start: 913014

End: 913994

Strand: Reverse

Name: rffG [C]

Synonym: Cmaq_0868

Alternate gene names: 159041441

Gene position: 913994-913014 (Counterclockwise)

Preceding gene: 159041443

Following gene: 159041440

Centisome position: 43.99

GC content: 44.24

Gene sequence:

>981_bases
ATGCCTGAATCAATCCTAGTTACTGGTGCCAGTGGTCAAGTTGGTGGTTTCACCGTTGGGGAGTTGATTAACATGGGTTA
TAGGGTTATTGCCCTTGATGTTAGGTTTAGTGATGAGTTAATTAGATTGAGGGGGCCTAGCCTTGAGTTGGTTAATGCGG
ATTTAAGTGATTTTGATGAGCTCATTAGTATTATTAAGAGGTTTAACGTTAGGCGTATTATTCACTTAGCTGCGATGATA
CTTCTTGAATCAAGGAATAGGCCCCTTAAGGCTGCTAAGGTTAATATCATCGGTACATTGAATGTTTTCGAGGCCGCTAG
GTTAATGGACCTTGAACGCGTTGTTTACGCAAGCTCAGAGTCTGTTTACGGTTCACCATTGGTTTACGGTAAGGGTAGTG
TTAATGAGGATGATTACCCCCATACACCGCCTGACCCATACCACATAACTAAGCTTGCCGATGAGTTATTCGGATCATAC
TACAGTGAGGCTTATGGCTTAAGCGTGATTGGGGGTAGGTTAACGACCGCGTGGGGTCCAGGTAGGTATAGTGGCTACAC
CGGCCAGTTTAATAGTTTCCTAAGGGATGTTATAATTAAGGGTTACGGTAAAGTACCTCCGGACTTCGCCTACAGTGGGG
CTAAGTATAGGTGGCTTTACGTTAAGGATGCTGCAAGGGCCTTTATTCACCTAGCCCTAGTGGATAAGGCTAAGGTTAGG
AGACCAGTCTACAATACTGGTTCAATGAAGCCGTTCACGGTAATTGACGTTATTAACACCATTAAGGAACTGATACCGAA
CGCTAGGATTGATTACGAACCATTAAGCAAACCCACTGAAACATCATCAAGGGTACCAGGCCCAGCAGGCCTAGACGTAG
ACTGTAGTAGACTATACGATGAATTAGGCTTTAGTGAGAGGTACGGCTTAAAAGGTGGCTTAATTGATATGATTGAGTAT
GAGAAGTCGAGAGCCAAGTGA

Upstream 100 bases:

>100_bases
CACCAGTCATATTAAGGCTCATCACACTTACCTTATTAAGCTTACGTGGCACTAGTATTTATGGTATTAATACTTAATAA
CCTAGTTAAGTAGCTTACGC

Downstream 100 bases:

>100_bases
ATCTAATAGGACTCATGATCATAAGCTATTAAGTTATTGATTAATAACGACAGTGCAGTAAATGTTATTGCTTAGAGAGT
AATCTCAAGGTTTTATGCAT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPESILVTGASGQVGGFTVGELINMGYRVIALDVRFSDELIRLRGPSLELVNADLSDFDELISIIKRFNVRRIIHLAAMI
LLESRNRPLKAAKVNIIGTLNVFEAARLMDLERVVYASSESVYGSPLVYGKGSVNEDDYPHTPPDPYHITKLADELFGSY
YSEAYGLSVIGGRLTTAWGPGRYSGYTGQFNSFLRDVIIKGYGKVPPDFAYSGAKYRWLYVKDAARAFIHLALVDKAKVR
RPVYNTGSMKPFTVIDVINTIKELIPNARIDYEPLSKPTETSSRVPGPAGLDVDCSRLYDELGFSERYGLKGGLIDMIEY
EKSRAK

Sequences:

>Translated_326_residues
MPESILVTGASGQVGGFTVGELINMGYRVIALDVRFSDELIRLRGPSLELVNADLSDFDELISIIKRFNVRRIIHLAAMI
LLESRNRPLKAAKVNIIGTLNVFEAARLMDLERVVYASSESVYGSPLVYGKGSVNEDDYPHTPPDPYHITKLADELFGSY
YSEAYGLSVIGGRLTTAWGPGRYSGYTGQFNSFLRDVIIKGYGKVPPDFAYSGAKYRWLYVKDAARAFIHLALVDKAKVR
RPVYNTGSMKPFTVIDVINTIKELIPNARIDYEPLSKPTETSSRVPGPAGLDVDCSRLYDELGFSERYGLKGGLIDMIEY
EKSRAK
>Mature_325_residues
PESILVTGASGQVGGFTVGELINMGYRVIALDVRFSDELIRLRGPSLELVNADLSDFDELISIIKRFNVRRIIHLAAMIL
LESRNRPLKAAKVNIIGTLNVFEAARLMDLERVVYASSESVYGSPLVYGKGSVNEDDYPHTPPDPYHITKLADELFGSYY
SEAYGLSVIGGRLTTAWGPGRYSGYTGQFNSFLRDVIIKGYGKVPPDFAYSGAKYRWLYVKDAARAFIHLALVDKAKVRR
PVYNTGSMKPFTVIDVINTIKELIPNARIDYEPLSKPTETSSRVPGPAGLDVDCSRLYDELGFSERYGLKGGLIDMIEYE
KSRAK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=281, Percent_Identity=25.6227758007117, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56237023, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI56118217, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI189083684, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788366, Length=196, Percent_Identity=29.5918367346939, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115532424, Length=276, Percent_Identity=22.1014492753623, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=137, Percent_Identity=32.8467153284672, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=135, Percent_Identity=34.0740740740741, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24667531, Length=297, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19923002, Length=138, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36241; Mature: 36110

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESILVTGASGQVGGFTVGELINMGYRVIALDVRFSDELIRLRGPSLELVNADLSDFDE
CCCCEEEECCCCCCCCEEHHHHHHCCEEEEEEEEEECCCCCEECCCCEEEEECCHHHHHH
LISIIKRFNVRRIIHLAAMILLESRNRPLKAAKVNIIGTLNVFEAARLMDLERVVYASSE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCC
SVYGSPLVYGKGSVNEDDYPHTPPDPYHITKLADELFGSYYSEAYGLSVIGGRLTTAWGP
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCC
GRYSGYTGQFNSFLRDVIIKGYGKVPPDFAYSGAKYRWLYVKDAARAFIHLALVDKAKVR
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHC
RPVYNTGSMKPFTVIDVINTIKELIPNARIDYEPLSKPTETSSRVPGPAGLDVDCSRLYD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ELGFSERYGLKGGLIDMIEYEKSRAK
HHCCCHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PESILVTGASGQVGGFTVGELINMGYRVIALDVRFSDELIRLRGPSLELVNADLSDFDE
CCCEEEECCCCCCCCEEHHHHHHCCEEEEEEEEEECCCCCEECCCCEEEEECCHHHHHH
LISIIKRFNVRRIIHLAAMILLESRNRPLKAAKVNIIGTLNVFEAARLMDLERVVYASSE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCC
SVYGSPLVYGKGSVNEDDYPHTPPDPYHITKLADELFGSYYSEAYGLSVIGGRLTTAWGP
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCC
GRYSGYTGQFNSFLRDVIIKGYGKVPPDFAYSGAKYRWLYVKDAARAFIHLALVDKAKVR
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHC
RPVYNTGSMKPFTVIDVINTIKELIPNARIDYEPLSKPTETSSRVPGPAGLDVDCSRLYD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ELGFSERYGLKGGLIDMIEYEKSRAK
HHCCCHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]