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The map label for this gene is cobB [H]

Identifier: 159041377

GI number: 159041377

Start: 839246

End: 840580

Strand: Reverse

Name: cobB [H]

Synonym: Cmaq_0804

Alternate gene names: 159041377

Gene position: 840580-839246 (Counterclockwise)

Preceding gene: 159041378

Following gene: 159041370

Centisome position: 40.46

GC content: 40.37

Gene sequence:

>1335_bases
ATGATGCCCACTGGCAGGGCGTTATTAATAGCATCGGATAGGAGTGGATCAGGAAAGACCACGGTTACATCAATAATAGC
AGCAGCATTATCAAGTAAACTTAAGGTTAGGGCGTTTAAGGTTGGGCCAGATTACATTGATCCAGGTTACCATAAAGTAG
CCACTGAATTACCCTCAATTAACCTTGATTTATGGTTAATGGGTGAGGAGAAGCTTAAAGAAACCTTCTGCAGATACATG
AGGGGGGTTGATTTAGGGTTGATTGAGGGTGTAATGGGATTATATGATGGTGTTGAGGTTGGGTCGAGTACATTTCATGT
TGCTAAATTACTCTCATTACCAGTTATCCTAGTAATAGACTGCTCCAACATATCCTATACAGCCGGGGCAATTGTTAAGG
GTTTAAGGGATTATGGACCTGTTAACGTGGTTGGGGTCATCTTCAATAATGTGGCTTCAAGCAACCACTATAACGCATGT
AAATCCTCACTACCCAAGGATGTAGTGAGCTTGGGTTACATACCATATGATGCAGGGTTAATCATTAATGAGAGGCATTT
AGGCCTTGTGACCGTGGAGGATTACGGTAATAAGGTTAAAGGGATTATTAAACGCGGCGTTGAGTTAATTAATGGGAAAA
TTAACCTTGATTTAATATATGATTTAGCTGAACCAGTTAACTGTGAACATGTTAATGAACAGGAGGATTACGGTAATAAA
CTTGGGGTTGCTGCAGTAGCCTACGATAACGCCTTCCTATTCTACTATAGGGCATCCCTTGATTTACTCTCAAGTATATT
TGACTTAAGGTTCTTTAGTCCAATAAATAATGAAGTAGTTAATAATGCATCATTCATATACATTGGTGGTGGATACCCTG
AACTGCATTTAAGGGAACTTGAGGCAGCTAGGGATACTGCCTCATGGTTAAGGAAGGCTGCATATGATGGTGTACCTATT
TTAGCTGAGTGCGGTGGTTTAATGTACTTATCAAAGTACATTAGGGATGATAGGGAGTACAGTATGGTTGGCTTATTTGA
CATTGGTATTGCCGCTAAGGATAGATTAACAATTGGATATACTGAACTTGAGAGCATTGTAGATAATCCACTAGTGCCCG
CTGGTCTAAGGATTAGGGGTCATGAGTTCCATGTTTCACGCGCAGAATACTCTAATGAAAGGTACGTGTTTAGGAATATT
AGGGGTAGAGGAATAATGAATAGTTACGATGGGGTCTTGTTAAATAATGCACTAGGCCTATACACCCACACGTACTTACC
ATCAATACCAGGCGTGAGGGATAGGATAATTAATATTGGGAAGCACTTGAGGTAA

Upstream 100 bases:

>100_bases
AGGTGACTTTAATGAACCAAGTGCTAGTGAAGTAATTAAGTATATTAAGGGTATTAATCCCTCCTGAGAAGCTTATTAAT
AATCCTCACCTTAACCGGTA

Downstream 100 bases:

>100_bases
TTCGCCTCCTCACCCCTTGACGTACAACCAGCAGGCTACAACATGACCATTCTCAAGGGTTACTAATGGCGGCTCCTCCC
TACGGCAAATATCCATAGCA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MMPTGRALLIASDRSGSGKTTVTSIIAAALSSKLKVRAFKVGPDYIDPGYHKVATELPSINLDLWLMGEEKLKETFCRYM
RGVDLGLIEGVMGLYDGVEVGSSTFHVAKLLSLPVILVIDCSNISYTAGAIVKGLRDYGPVNVVGVIFNNVASSNHYNAC
KSSLPKDVVSLGYIPYDAGLIINERHLGLVTVEDYGNKVKGIIKRGVELINGKINLDLIYDLAEPVNCEHVNEQEDYGNK
LGVAAVAYDNAFLFYYRASLDLLSSIFDLRFFSPINNEVVNNASFIYIGGGYPELHLRELEAARDTASWLRKAAYDGVPI
LAECGGLMYLSKYIRDDREYSMVGLFDIGIAAKDRLTIGYTELESIVDNPLVPAGLRIRGHEFHVSRAEYSNERYVFRNI
RGRGIMNSYDGVLLNNALGLYTHTYLPSIPGVRDRIINIGKHLR

Sequences:

>Translated_444_residues
MMPTGRALLIASDRSGSGKTTVTSIIAAALSSKLKVRAFKVGPDYIDPGYHKVATELPSINLDLWLMGEEKLKETFCRYM
RGVDLGLIEGVMGLYDGVEVGSSTFHVAKLLSLPVILVIDCSNISYTAGAIVKGLRDYGPVNVVGVIFNNVASSNHYNAC
KSSLPKDVVSLGYIPYDAGLIINERHLGLVTVEDYGNKVKGIIKRGVELINGKINLDLIYDLAEPVNCEHVNEQEDYGNK
LGVAAVAYDNAFLFYYRASLDLLSSIFDLRFFSPINNEVVNNASFIYIGGGYPELHLRELEAARDTASWLRKAAYDGVPI
LAECGGLMYLSKYIRDDREYSMVGLFDIGIAAKDRLTIGYTELESIVDNPLVPAGLRIRGHEFHVSRAEYSNERYVFRNI
RGRGIMNSYDGVLLNNALGLYTHTYLPSIPGVRDRIINIGKHLR
>Mature_444_residues
MMPTGRALLIASDRSGSGKTTVTSIIAAALSSKLKVRAFKVGPDYIDPGYHKVATELPSINLDLWLMGEEKLKETFCRYM
RGVDLGLIEGVMGLYDGVEVGSSTFHVAKLLSLPVILVIDCSNISYTAGAIVKGLRDYGPVNVVGVIFNNVASSNHYNAC
KSSLPKDVVSLGYIPYDAGLIINERHLGLVTVEDYGNKVKGIIKRGVELINGKINLDLIYDLAEPVNCEHVNEQEDYGNK
LGVAAVAYDNAFLFYYRASLDLLSSIFDLRFFSPINNEVVNNASFIYIGGGYPELHLRELEAARDTASWLRKAAYDGVPI
LAECGGLMYLSKYIRDDREYSMVGLFDIGIAAKDRLTIGYTELESIVDNPLVPAGLRIRGHEFHVSRAEYSNERYVFRNI
RGRGIMNSYDGVLLNNALGLYTHTYLPSIPGVRDRIINIGKHLR

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 49038; Mature: 49038

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPTGRALLIASDRSGSGKTTVTSIIAAALSSKLKVRAFKVGPDYIDPGYHKVATELPSI
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHCCCC
NLDLWLMGEEKLKETFCRYMRGVDLGLIEGVMGLYDGVEVGSSTFHVAKLLSLPVILVID
CEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCHHHHHHHHCCCEEEEEE
CSNISYTAGAIVKGLRDYGPVNVVGVIFNNVASSNHYNACKSSLPKDVVSLGYIPYDAGL
CCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCEECCCE
IINERHLGLVTVEDYGNKVKGIIKRGVELINGKINLDLIYDLAEPVNCEHVNEQEDYGNK
EEECCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCHHHCCCC
LGVAAVAYDNAFLFYYRASLDLLSSIFDLRFFSPINNEVVNNASFIYIGGGYPELHLREL
CCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHH
EAARDTASWLRKAAYDGVPILAECGGLMYLSKYIRDDREYSMVGLFDIGIAAKDRLTIGY
HHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECH
TELESIVDNPLVPAGLRIRGHEFHVSRAEYSNERYVFRNIRGRGIMNSYDGVLLNNALGL
HHHHHHHCCCCCCCCEEEECCEEEEEHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHH
YTHTYLPSIPGVRDRIINIGKHLR
HHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMPTGRALLIASDRSGSGKTTVTSIIAAALSSKLKVRAFKVGPDYIDPGYHKVATELPSI
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHCCCC
NLDLWLMGEEKLKETFCRYMRGVDLGLIEGVMGLYDGVEVGSSTFHVAKLLSLPVILVID
CEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCHHHHHHHHCCCEEEEEE
CSNISYTAGAIVKGLRDYGPVNVVGVIFNNVASSNHYNACKSSLPKDVVSLGYIPYDAGL
CCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCEECCCE
IINERHLGLVTVEDYGNKVKGIIKRGVELINGKINLDLIYDLAEPVNCEHVNEQEDYGNK
EEECCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCHHHCCCC
LGVAAVAYDNAFLFYYRASLDLLSSIFDLRFFSPINNEVVNNASFIYIGGGYPELHLREL
CCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHH
EAARDTASWLRKAAYDGVPILAECGGLMYLSKYIRDDREYSMVGLFDIGIAAKDRLTIGY
HHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECH
TELESIVDNPLVPAGLRIRGHEFHVSRAEYSNERYVFRNIRGRGIMNSYDGVLLNNALGL
HHHHHHHCCCCCCCCEEEECCEEEEEHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHH
YTHTYLPSIPGVRDRIINIGKHLR
HHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]