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The map label for this gene is purF [H]

Identifier: 159041244

GI number: 159041244

Start: 701327

End: 702550

Strand: Reverse

Name: purF [H]

Synonym: Cmaq_0666

Alternate gene names: 159041244

Gene position: 702550-701327 (Counterclockwise)

Preceding gene: 159041245

Following gene: 159041243

Centisome position: 33.82

GC content: 45.92

Gene sequence:

>1224_bases
ATGGGTGTAATCGGGGTTGTGGGTGTATTATCCTTCCACGAGGGCTGGGATGTGGTTAAGGTTATTAAATACCCATTAAT
GGCGCTTAAGCATAGGGGTAATGGGCGATACTTCATAGCCGTCTTAAGTGATAATTTACGTACAGGGCTTGTTAATGACC
TTGAATTCAAGGGATTCAACGGGTGGGTGGCCTTGGCCTGCACCTACATTGATGAATCATCAGTGGGGGTTACTAAATGC
GGTGACGTAACTGTAGCTTACTGTGTTGAGGGACAGGAATTGAAACCAGAGGATATATGCGATCTAGTTAGCAATGGTAA
GCAACCTAAGGCTCAATCATCGGTTATTGCCTTAACAAGCGATGGTAGATTAATGGGCCTAAGACCCAGTAGTGGGTTGA
GAAACCTAGCCCTAGGTGCCTACGGTTTCGACTTAGCCATAATATCTAATGAAACCTCAGCCATTAACGCCATGGGTGGT
TCGGTGAGGAGGTTCATTGAACCAGGGGCATTAGTTACGATTAGTAGGAATAATGTTGAGTTTAACGGGGGCGTGGGTAA
AGCAGGTAAGGTGTGTTCACTGGAGTTAATCTACATGGCTAGACCTGACTCTGAGGTGGATGGACACTCAGTGTATGCCT
TTAGGGTTAATTTAGCTAAGAGAATGGCTAAGGGGATTAAGGTTAACGTTGACTCAGTGGTTGGGGTTCCTGAAACCGGT
GTCCTCTATGCCGTTAAGGTTGGTGAATCACTGGGTAAGCCTGTTGAACTTGCCTTAAGCATTATTGAGAGGCGTAGATC
AGCATTAACCAGTGAATTGATTGATAAACTATCATCAATACACCTTAAGGTTAACCCAGTCATCAACGCCATTAGGGGGA
GGAAACTCCTCCTAATTGATGACTCACTGGTAAGCGGCTTAACAATTAAGGAAATATCCCAGGCCCTTAGGCATAGGGCA
GGGGTTAAGGAGATTCACGTAGCCATAGCCAGCCCAAGGATAGTGAGGCGTTGCCCATACGGCGTCAACATGCCTCCCGA
TGAGCAATTACTGGCTAATGTATTCCCTAATGACGAGGACTTAGTTAAGTTACTTGAGGTTGATTCAATATCCTGGCTTC
AACTAAGTGACTTAATCAAGGCTGCGGAGGATGTAGGGATAGGGAAGGGGAACCTATGCCTAGAATGCTTCCTGAGGAGG
GACCATGAAGTGGACTTACAGTAA

Upstream 100 bases:

>100_bases
CCATAGGCACGGTGGAATTATGTACAGCATGCTTCACAGGGGAGTACCCGTTTAAGGTTAACATTGATGAATTAGAGGGG
GCCTTCACGGGTGATAGGTA

Downstream 100 bases:

>100_bases
AGCTGGGGTGGATTTAAGTAAACACCACAGCATGCACTCAATAGCCCAGGACGCGATTAGGCGTATTGAGGAATCCCTTG
GAGTAAAGTTAATTGAGGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MGVIGVVGVLSFHEGWDVVKVIKYPLMALKHRGNGRYFIAVLSDNLRTGLVNDLEFKGFNGWVALACTYIDESSVGVTKC
GDVTVAYCVEGQELKPEDICDLVSNGKQPKAQSSVIALTSDGRLMGLRPSSGLRNLALGAYGFDLAIISNETSAINAMGG
SVRRFIEPGALVTISRNNVEFNGGVGKAGKVCSLELIYMARPDSEVDGHSVYAFRVNLAKRMAKGIKVNVDSVVGVPETG
VLYAVKVGESLGKPVELALSIIERRRSALTSELIDKLSSIHLKVNPVINAIRGRKLLLIDDSLVSGLTIKEISQALRHRA
GVKEIHVAIASPRIVRRCPYGVNMPPDEQLLANVFPNDEDLVKLLEVDSISWLQLSDLIKAAEDVGIGKGNLCLECFLRR
DHEVDLQ

Sequences:

>Translated_407_residues
MGVIGVVGVLSFHEGWDVVKVIKYPLMALKHRGNGRYFIAVLSDNLRTGLVNDLEFKGFNGWVALACTYIDESSVGVTKC
GDVTVAYCVEGQELKPEDICDLVSNGKQPKAQSSVIALTSDGRLMGLRPSSGLRNLALGAYGFDLAIISNETSAINAMGG
SVRRFIEPGALVTISRNNVEFNGGVGKAGKVCSLELIYMARPDSEVDGHSVYAFRVNLAKRMAKGIKVNVDSVVGVPETG
VLYAVKVGESLGKPVELALSIIERRRSALTSELIDKLSSIHLKVNPVINAIRGRKLLLIDDSLVSGLTIKEISQALRHRA
GVKEIHVAIASPRIVRRCPYGVNMPPDEQLLANVFPNDEDLVKLLEVDSISWLQLSDLIKAAEDVGIGKGNLCLECFLRR
DHEVDLQ
>Mature_406_residues
GVIGVVGVLSFHEGWDVVKVIKYPLMALKHRGNGRYFIAVLSDNLRTGLVNDLEFKGFNGWVALACTYIDESSVGVTKCG
DVTVAYCVEGQELKPEDICDLVSNGKQPKAQSSVIALTSDGRLMGLRPSSGLRNLALGAYGFDLAIISNETSAINAMGGS
VRRFIEPGALVTISRNNVEFNGGVGKAGKVCSLELIYMARPDSEVDGHSVYAFRVNLAKRMAKGIKVNVDSVVGVPETGV
LYAVKVGESLGKPVELALSIIERRRSALTSELIDKLSSIHLKVNPVINAIRGRKLLLIDDSLVSGLTIKEISQALRHRAG
VKEIHVAIASPRIVRRCPYGVNMPPDEQLLANVFPNDEDLVKLLEVDSISWLQLSDLIKAAEDVGIGKGNLCLECFLRRD
HEVDLQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=274, Percent_Identity=28.4671532846715, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788651, Length=289, Percent_Identity=28.719723183391, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17554892, Length=310, Percent_Identity=26.7741935483871, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323958, Length=293, Percent_Identity=27.6450511945392, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573187, Length=465, Percent_Identity=26.0215053763441, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24659598, Length=309, Percent_Identity=28.1553398058252, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24659604, Length=260, Percent_Identity=29.2307692307692, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 44075; Mature: 43943

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVIGVVGVLSFHEGWDVVKVIKYPLMALKHRGNGRYFIAVLSDNLRTGLVNDLEFKGFN
CCCEEHHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCCCEECCCC
GWVALACTYIDESSVGVTKCGDVTVAYCVEGQELKPEDICDLVSNGKQPKAQSSVIALTS
CEEEEEEEEECCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEC
DGRLMGLRPSSGLRNLALGAYGFDLAIISNETSAINAMGGSVRRFIEPGALVTISRNNVE
CCEEEEECCCCCCCHHEEEECCEEEEEEECCCCEEHHCCHHHHHHCCCCEEEEEECCCEE
FNGGVGKAGKVCSLELIYMARPDSEVDGHSVYAFRVNLAKRMAKGIKVNVDSVVGVPETG
ECCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEEEHHHHHHCCCEEEEHHEECCCCCC
VLYAVKVGESLGKPVELALSIIERRRSALTSELIDKLSSIHLKVNPVINAIRGRKLLLID
EEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHCCCEEEEEE
DSLVSGLTIKEISQALRHRAGVKEIHVAIASPRIVRRCPYGVNMPPDEQLLANVFPNDED
CHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCHHHHHHCCCCHHH
LVKLLEVDSISWLQLSDLIKAAEDVGIGKGNLCLECFLRRDHEVDLQ
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GVIGVVGVLSFHEGWDVVKVIKYPLMALKHRGNGRYFIAVLSDNLRTGLVNDLEFKGFN
CCEEHHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCCCEECCCC
GWVALACTYIDESSVGVTKCGDVTVAYCVEGQELKPEDICDLVSNGKQPKAQSSVIALTS
CEEEEEEEEECCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEC
DGRLMGLRPSSGLRNLALGAYGFDLAIISNETSAINAMGGSVRRFIEPGALVTISRNNVE
CCEEEEECCCCCCCHHEEEECCEEEEEEECCCCEEHHCCHHHHHHCCCCEEEEEECCCEE
FNGGVGKAGKVCSLELIYMARPDSEVDGHSVYAFRVNLAKRMAKGIKVNVDSVVGVPETG
ECCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEEEEHHHHHHCCCEEEEHHEECCCCCC
VLYAVKVGESLGKPVELALSIIERRRSALTSELIDKLSSIHLKVNPVINAIRGRKLLLID
EEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHCCCEEEEEE
DSLVSGLTIKEISQALRHRAGVKEIHVAIASPRIVRRCPYGVNMPPDEQLLANVFPNDED
CHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCHHHHHHCCCCHHH
LVKLLEVDSISWLQLSDLIKAAEDVGIGKGNLCLECFLRRDHEVDLQ
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]