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The map label for this gene is purF [H]

Identifier: 159041245

GI number: 159041245

Start: 702550

End: 703851

Strand: Reverse

Name: purF [H]

Synonym: Cmaq_0667

Alternate gene names: 159041245

Gene position: 703851-702550 (Counterclockwise)

Preceding gene: 159041246

Following gene: 159041244

Centisome position: 33.88

GC content: 45.31

Gene sequence:

>1302_bases
ATGTGCGGCATATGGGCTTACCTAGGTCAAGGAGCAAACCTAATGGTGAGCAAAATGGCGCCATGGCTAATGCATAGAGG
GCAGGAGGGATTCTCATACGTCTGCGTTCGTAATGGGGCTTTACTTAAGGTCAATGACCCAATACCACTGGAGTCTAATC
TCTGCCTTGGACACGCCAGGTACTCCACCAGTGGACCATACGGTGTTGAGCTTCAACCAGTGGTTTTAGGGGACTTGGCG
TTAGTCTTCAATGGTACAGTGGCTAATTATAAGGAATTGAAAATGAGGTTAAGGGAAATGGGGATTATCGTTAATTCTAA
TTATGATGCATTAATACTGGCTCAATACCTTAAGAACCTATTAACTAGGCTAAGTATTGATGATACTGTTAATGAAGTCT
TCAAGACCATTAAGGGTGGTTACTCGATACTGGCTCTTTGGGGTAATTCACTAATAGCTATTAGGGATCCATGGGGATTA
AGACCGCTTGCAATGGGAATCACTAATGATGGTGTAGTATTTGCCTCAGAAACATCAGTCCTAGATGCCTTAGGCATTAA
GTGGATTGAGGTTAAGCCAGGTAACGCCCTGGTGCTGGGTAGTAATGGGGAGAGGATATTGAATTGGCCTAGTGTTAGAA
GAATGTACTGCGCCCTGGAGTATATTTACTTCCAAAGACCTGACTCAGTTTTTAACGGCATAAGCATCTATAGTGCTAGG
CGTAGGCTTGGGTTAGCGTTAGCGAGGAAGGAGGGGGAGGAGGTTGATGAGGTTTCCCCAATCCCAGAAACCGCCAGGGT
TGCTGCGCAAGCATATGCTAATGCCCTTGGTAAGCCATTGAATGAGTTCATAGTTAAGAATAGGTTCATGGGGAGGGGAT
TCATAAAACCGCCTAAGGATAGGGACTTTGAACTATACAGTGTAATTAAGGAGGGGGTGACTGGTAAGTCAGTGGCCTTA
ATTGATGACTCAATAATAAGGGGTACTACGCTTAGGAGGATAATACCTAAGGTTAAGGCCGCTGGAGCTAAGGCAATTCA
CGTAAGGGTTTCTTCACCGCCCGTGAGGTACCCATGCTTCATGGGGATGGACTTCCCAAGTAGGCGTGAATTAATAGCTC
ATGGTAAATCCATAGGGGAAGTTAAGTCAATGCTGGGTTCAGATTCACTAACCTACTTAACCGTTGATGAACTTAAGGAG
GCCATAGGCACGGTGGAATTATGTACAGCATGCTTCACAGGGGAGTACCCGTTTAAGGTTAACATTGATGAATTAGAGGG
GGCCTTCACGGGTGATAGGTAA

Upstream 100 bases:

>100_bases
GGGGAGGGATAGTGCAGTTTAAGTGGGGTGGCTTGGAGTTAGCATCCCTGAGTGTTGATGACTTAGTTAGTATTAATGAT
TCCTTGAGGGGTGTCATATA

Downstream 100 bases:

>100_bases
TGGGTGTAATCGGGGTTGTGGGTGTATTATCCTTCCACGAGGGCTGGGATGTGGTTAAGGTTATTAAATACCCATTAATG
GCGCTTAAGCATAGGGGTAA

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MCGIWAYLGQGANLMVSKMAPWLMHRGQEGFSYVCVRNGALLKVNDPIPLESNLCLGHARYSTSGPYGVELQPVVLGDLA
LVFNGTVANYKELKMRLREMGIIVNSNYDALILAQYLKNLLTRLSIDDTVNEVFKTIKGGYSILALWGNSLIAIRDPWGL
RPLAMGITNDGVVFASETSVLDALGIKWIEVKPGNALVLGSNGERILNWPSVRRMYCALEYIYFQRPDSVFNGISIYSAR
RRLGLALARKEGEEVDEVSPIPETARVAAQAYANALGKPLNEFIVKNRFMGRGFIKPPKDRDFELYSVIKEGVTGKSVAL
IDDSIIRGTTLRRIIPKVKAAGAKAIHVRVSSPPVRYPCFMGMDFPSRRELIAHGKSIGEVKSMLGSDSLTYLTVDELKE
AIGTVELCTACFTGEYPFKVNIDELEGAFTGDR

Sequences:

>Translated_433_residues
MCGIWAYLGQGANLMVSKMAPWLMHRGQEGFSYVCVRNGALLKVNDPIPLESNLCLGHARYSTSGPYGVELQPVVLGDLA
LVFNGTVANYKELKMRLREMGIIVNSNYDALILAQYLKNLLTRLSIDDTVNEVFKTIKGGYSILALWGNSLIAIRDPWGL
RPLAMGITNDGVVFASETSVLDALGIKWIEVKPGNALVLGSNGERILNWPSVRRMYCALEYIYFQRPDSVFNGISIYSAR
RRLGLALARKEGEEVDEVSPIPETARVAAQAYANALGKPLNEFIVKNRFMGRGFIKPPKDRDFELYSVIKEGVTGKSVAL
IDDSIIRGTTLRRIIPKVKAAGAKAIHVRVSSPPVRYPCFMGMDFPSRRELIAHGKSIGEVKSMLGSDSLTYLTVDELKE
AIGTVELCTACFTGEYPFKVNIDELEGAFTGDR
>Mature_433_residues
MCGIWAYLGQGANLMVSKMAPWLMHRGQEGFSYVCVRNGALLKVNDPIPLESNLCLGHARYSTSGPYGVELQPVVLGDLA
LVFNGTVANYKELKMRLREMGIIVNSNYDALILAQYLKNLLTRLSIDDTVNEVFKTIKGGYSILALWGNSLIAIRDPWGL
RPLAMGITNDGVVFASETSVLDALGIKWIEVKPGNALVLGSNGERILNWPSVRRMYCALEYIYFQRPDSVFNGISIYSAR
RRLGLALARKEGEEVDEVSPIPETARVAAQAYANALGKPLNEFIVKNRFMGRGFIKPPKDRDFELYSVIKEGVTGKSVAL
IDDSIIRGTTLRRIIPKVKAAGAKAIHVRVSSPPVRYPCFMGMDFPSRRELIAHGKSIGEVKSMLGSDSLTYLTVDELKE
AIGTVELCTACFTGEYPFKVNIDELEGAFTGDR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=432, Percent_Identity=32.8703703703704, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1788651, Length=449, Percent_Identity=32.9621380846325, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=29.9376299376299, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=31.1608961303462, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=34.6473029045643, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24659604, Length=454, Percent_Identity=35.4625550660793, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI28573187, Length=479, Percent_Identity=33.6116910229645, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 47770; Mature: 47770

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIWAYLGQGANLMVSKMAPWLMHRGQEGFSYVCVRNGALLKVNDPIPLESNLCLGHAR
CCCEEHHHCCCCCEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEECC
YSTSGPYGVELQPVVLGDLALVFNGTVANYKELKMRLREMGIIVNSNYDALILAQYLKNL
CCCCCCCCEEEEEEEECCEEEEECCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
LTRLSIDDTVNEVFKTIKGGYSILALWGNSLIAIRDPWGLRPLAMGITNDGVVFASETSV
HHHCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCEEEEECCHH
LDALGIKWIEVKPGNALVLGSNGERILNWPSVRRMYCALEYIYFQRPDSVFNGISIYSAR
HHHHCCEEEEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHEECCHHHHCCHHHHHHH
RRLGLALARKEGEEVDEVSPIPETARVAAQAYANALGKPLNEFIVKNRFMGRGFIKPPKD
HHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
RDFELYSVIKEGVTGKSVALIDDSIIRGTTLRRIIPKVKAAGAKAIHVRVSSPPVRYPCF
CCHHHHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
MGMDFPSRRELIAHGKSIGEVKSMLGSDSLTYLTVDELKEAIGTVELCTACFTGEYPFKV
ECCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
NIDELEGAFTGDR
EHHHHCCCCCCCC
>Mature Secondary Structure
MCGIWAYLGQGANLMVSKMAPWLMHRGQEGFSYVCVRNGALLKVNDPIPLESNLCLGHAR
CCCEEHHHCCCCCEEHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEECC
YSTSGPYGVELQPVVLGDLALVFNGTVANYKELKMRLREMGIIVNSNYDALILAQYLKNL
CCCCCCCCEEEEEEEECCEEEEECCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
LTRLSIDDTVNEVFKTIKGGYSILALWGNSLIAIRDPWGLRPLAMGITNDGVVFASETSV
HHHCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCEEEEECCHH
LDALGIKWIEVKPGNALVLGSNGERILNWPSVRRMYCALEYIYFQRPDSVFNGISIYSAR
HHHHCCEEEEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHEECCHHHHCCHHHHHHH
RRLGLALARKEGEEVDEVSPIPETARVAAQAYANALGKPLNEFIVKNRFMGRGFIKPPKD
HHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
RDFELYSVIKEGVTGKSVALIDDSIIRGTTLRRIIPKVKAAGAKAIHVRVSSPPVRYPCF
CCHHHHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
MGMDFPSRRELIAHGKSIGEVKSMLGSDSLTYLTVDELKEAIGTVELCTACFTGEYPFKV
ECCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
NIDELEGAFTGDR
EHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]