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The map label for this gene is truB [H]

Identifier: 159040742

GI number: 159040742

Start: 181704

End: 182711

Strand: Reverse

Name: truB [H]

Synonym: Cmaq_0155

Alternate gene names: 159040742

Gene position: 182711-181704 (Counterclockwise)

Preceding gene: 159040743

Following gene: 159040740

Centisome position: 8.79

GC content: 45.34

Gene sequence:

>1008_bases
ATGTTTAATGTACTTAACTGTAGTGGTGAAAGGATCATTAAGGTTAAGGTGGATGAGGGTACTGACCCAAGGTATGGTGT
TAATCCTTATGAAAGAAGGATTGAGGAGTACATTAACTACGGTTTCATAATCCTTGATAAACCAAGGGGGCCGACTAGTA
GTGAGGTTGTTGCATGGGTTAAGAAGCTGCTTAATATCAGTAAGGCTGGGCACTCTGGTACACTTGATCCAGGGGTTTCT
GGGGTTCTCCCAATAGCTCTGGGTGATTCAACTAAGGTATTGCAAGGTATAAGTAATGTTGATAAGGAGTACGTGGGTGT
TATGCTCCTTCACTCTACTGTGGATCAGGAGAGGGTTAAGGCTGTCTTCAGGGAGTTCACGGGTAAAATATATCAACGCC
CACCCCTCAAGAGTGCTGTTAAGAGGAGGATTAGGGTTAAGACGGTTTATAGCCTGGATATAATGGAATTCGACGGTAGG
TACGTTTTGTTTAAGGCTGATGTTGAGTCAGGGACTTACATTAGGAAACTATGCTACGATATTGGTGAGGTTTTAGGTGT
TGGTGCAAGCATGAGGGAGTTAAGGAGGACTAGGGTCGGTTGCTTCAGGGAGGAGAATACAGTTAACTTGAATACGTTAA
GGGAAGCTTACTCACTTTGGAGGGATTACGGTGATGAATCACTGCTTAGGAGGTTAATTAGGCCTGTTGAGGAAATGGTG
ATTCACCTACCTAGAATCTACATTAGGGACTCCGCAGTGGATGCAATAGCCCACGGTGCATCACTGGCGGCACCGGGGGT
TGCTAAGGTTGAGGAGGGTATTAGGAGTGGTCAATTAGTAGCCTTAATGACGCTTAAGGGTGAGTTAGTGGCGTTGGCTG
AATCCACTGCCTCAACTAATGAGATACTTAACATGAATAGGGGGATTGTAGCTAAGCCAACTAGGGTTATTATGCCTAGG
GGTGTTTATCCATCAACCTGGAGGAGAACCAGTAAGCAGGCCACGTAG

Upstream 100 bases:

>100_bases
TTATGAAAGGGGCATGATGTTTCTTTTTTTAAGGTAGGGAAGTCTCCTTTGAACCCCTAACTTTTATAAAAATCCTTCAA
AGGGAAGGAGACTGTGGTTT

Downstream 100 bases:

>100_bases
CCTAATTAGGAGCCGTACCTCCTCCACGTACCCTTAATCACCCACACGGGCTCCTCCTCATGGAATGGTTCCATACCCGT
GAATCTCAACTCATCCAGTA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFNVLNCSGERIIKVKVDEGTDPRYGVNPYERRIEEYINYGFIILDKPRGPTSSEVVAWVKKLLNISKAGHSGTLDPGVS
GVLPIALGDSTKVLQGISNVDKEYVGVMLLHSTVDQERVKAVFREFTGKIYQRPPLKSAVKRRIRVKTVYSLDIMEFDGR
YVLFKADVESGTYIRKLCYDIGEVLGVGASMRELRRTRVGCFREENTVNLNTLREAYSLWRDYGDESLLRRLIRPVEEMV
IHLPRIYIRDSAVDAIAHGASLAAPGVAKVEEGIRSGQLVALMTLKGELVALAESTASTNEILNMNRGIVAKPTRVIMPR
GVYPSTWRRTSKQAT

Sequences:

>Translated_335_residues
MFNVLNCSGERIIKVKVDEGTDPRYGVNPYERRIEEYINYGFIILDKPRGPTSSEVVAWVKKLLNISKAGHSGTLDPGVS
GVLPIALGDSTKVLQGISNVDKEYVGVMLLHSTVDQERVKAVFREFTGKIYQRPPLKSAVKRRIRVKTVYSLDIMEFDGR
YVLFKADVESGTYIRKLCYDIGEVLGVGASMRELRRTRVGCFREENTVNLNTLREAYSLWRDYGDESLLRRLIRPVEEMV
IHLPRIYIRDSAVDAIAHGASLAAPGVAKVEEGIRSGQLVALMTLKGELVALAESTASTNEILNMNRGIVAKPTRVIMPR
GVYPSTWRRTSKQAT
>Mature_335_residues
MFNVLNCSGERIIKVKVDEGTDPRYGVNPYERRIEEYINYGFIILDKPRGPTSSEVVAWVKKLLNISKAGHSGTLDPGVS
GVLPIALGDSTKVLQGISNVDKEYVGVMLLHSTVDQERVKAVFREFTGKIYQRPPLKSAVKRRIRVKTVYSLDIMEFDGR
YVLFKADVESGTYIRKLCYDIGEVLGVGASMRELRRTRVGCFREENTVNLNTLREAYSLWRDYGDESLLRRLIRPVEEMV
IHLPRIYIRDSAVDAIAHGASLAAPGVAKVEEGIRSGQLVALMTLKGELVALAESTASTNEILNMNRGIVAKPTRVIMPR
GVYPSTWRRTSKQAT

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI4503337, Length=306, Percent_Identity=42.156862745098, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI215599015, Length=306, Percent_Identity=42.156862745098, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI21040257, Length=218, Percent_Identity=29.3577981651376, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI2367200, Length=292, Percent_Identity=30.1369863013699, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17553978, Length=319, Percent_Identity=39.4984326018809, Blast_Score=258, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6323204, Length=306, Percent_Identity=42.156862745098, Blast_Score=254, Evalue=1e-68,
Organism=Drosophila melanogaster, GI281364189, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI281364187, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI281364185, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI281364183, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI62471759, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI17975520, Length=319, Percent_Identity=40.4388714733542, Blast_Score=257, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 37507; Mature: 37507

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNVLNCSGERIIKVKVDEGTDPRYGVNPYERRIEEYINYGFIILDKPRGPTSSEVVAWV
CCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KKLLNISKAGHSGTLDPGVSGVLPIALGDSTKVLQGISNVDKEYVGVMLLHSTVDQERVK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
AVFREFTGKIYQRPPLKSAVKRRIRVKTVYSLDIMEFDGRYVLFKADVESGTYIRKLCYD
HHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHH
IGEVLGVGASMRELRRTRVGCFREENTVNLNTLREAYSLWRDYGDESLLRRLIRPVEEMV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHLPRIYIRDSAVDAIAHGASLAAPGVAKVEEGIRSGQLVALMTLKGELVALAESTASTN
HHCCHHEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHH
EILNMNRGIVAKPTRVIMPRGVYPSTWRRTSKQAT
HHHHCCCCEEECCCEEEECCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MFNVLNCSGERIIKVKVDEGTDPRYGVNPYERRIEEYINYGFIILDKPRGPTSSEVVAWV
CCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
KKLLNISKAGHSGTLDPGVSGVLPIALGDSTKVLQGISNVDKEYVGVMLLHSTVDQERVK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHH
AVFREFTGKIYQRPPLKSAVKRRIRVKTVYSLDIMEFDGRYVLFKADVESGTYIRKLCYD
HHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHH
IGEVLGVGASMRELRRTRVGCFREENTVNLNTLREAYSLWRDYGDESLLRRLIRPVEEMV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IHLPRIYIRDSAVDAIAHGASLAAPGVAKVEEGIRSGQLVALMTLKGELVALAESTASTN
HHCCHHEEEHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHH
EILNMNRGIVAKPTRVIMPRGVYPSTWRRTSKQAT
HHHHCCCCEEECCCEEEECCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]