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The map label for this gene is thiE

Identifier: 159040653

GI number: 159040653

Start: 75444

End: 76061

Strand: Reverse

Name: thiE

Synonym: Cmaq_0061

Alternate gene names: 159040653

Gene position: 76061-75444 (Counterclockwise)

Preceding gene: 159040654

Following gene: 159040649

Centisome position: 3.66

GC content: 47.25

Gene sequence:

>618_bases
GTGAGATTACCTAAGGGCATATACGGTATAACTGATGACTCATATAATGTTAAGAACCATGTGGATGCAGCCAAGGTGTT
CCTAGAGGGGGGTGTGAGGATAATTCAGTATAGGCGTAAGGAGGGTAGCATAAGGCAGATGCTTAATGAGGCTAAGGAGA
TTAGGAAACTGTGTAATCAATATGGAGCAGTAATGATAGTTGATGATAGGGTTGACATAGCGGTACTCTCTGATGCTGAC
GGCGTCCACGTGGGTTTGGAGGATGCACCAGTGGATGAGGTTAAGAGGAGGTTTAGTGGAATTATAATCGGGGCAAGCGC
AAGCACTGTTGATGAGGCTAAGGAGGGGGAGAAGGCTGGTGCAGATTACTTAGGTGCAGGCTCCATATTCCCAAGCCCCA
CTAAACCGGACTACAGGATACTTGGACTTGAGGGATTAAGAAGAGTCGTCCAATCCGTTAGTATACCGGTATACGCAATA
GGTGGTGTAACCTTAGAATCCATACCAGCCATAAAGGCCACTGGGGCTTGGGGTGCCGCAGTGATTTCAGGAATATTAGC
GGCAAAGGATCCCTTGGAAATGGCTAAGAGGTTTGTTAAGGCTTGGGATGAGGCTTAA

Upstream 100 bases:

>100_bases
AGAGTAAGGCACCTGGTACATTTAAACAGTACCTAATTGATGAGTTATTTAACCTAACCGGTGACGATTACGTTAAAATG
GCTAAGGTAGAGGTGGTTCA

Downstream 100 bases:

>100_bases
TGACTCCATTACCCAATGCTTAGAAGTGGTGATCCTCATTTAATTCACCTGACTTGAGGGATAAGGTCTTAACTACCTAG
ATTTTAAGTAAAATTAACTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRLPKGIYGITDDSYNVKNHVDAAKVFLEGGVRIIQYRRKEGSIRQMLNEAKEIRKLCNQYGAVMIVDDRVDIAVLSDAD
GVHVGLEDAPVDEVKRRFSGIIIGASASTVDEAKEGEKAGADYLGAGSIFPSPTKPDYRILGLEGLRRVVQSVSIPVYAI
GGVTLESIPAIKATGAWGAAVISGILAAKDPLEMAKRFVKAWDEA

Sequences:

>Translated_205_residues
MRLPKGIYGITDDSYNVKNHVDAAKVFLEGGVRIIQYRRKEGSIRQMLNEAKEIRKLCNQYGAVMIVDDRVDIAVLSDAD
GVHVGLEDAPVDEVKRRFSGIIIGASASTVDEAKEGEKAGADYLGAGSIFPSPTKPDYRILGLEGLRRVVQSVSIPVYAI
GGVTLESIPAIKATGAWGAAVISGILAAKDPLEMAKRFVKAWDEA
>Mature_205_residues
MRLPKGIYGITDDSYNVKNHVDAAKVFLEGGVRIIQYRRKEGSIRQMLNEAKEIRKLCNQYGAVMIVDDRVDIAVLSDAD
GVHVGLEDAPVDEVKRRFSGIIIGASASTVDEAKEGEKAGADYLGAGSIFPSPTKPDYRILGLEGLRRVVQSVSIPVYAI
GGVTLESIPAIKATGAWGAAVISGILAAKDPLEMAKRFVKAWDEA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=183, Percent_Identity=31.6939890710383, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=191, Percent_Identity=30.3664921465969, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CALMQ (A8M9N4)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001539905.1
- ProteinModelPortal:   A8M9N4
- SMR:   A8M9N4
- GeneID:   5710154
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0061
- HOGENOM:   HBG754477
- OMA:   QEHIDKT
- BioCyc:   CMAQ397948:CMAQ_0061-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22058; Mature: 22058

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPKGIYGITDDSYNVKNHVDAAKVFLEGGVRIIQYRRKEGSIRQMLNEAKEIRKLCNQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEHHCCCHHHHHHHHHHHHHHHHHH
YGAVMIVDDRVDIAVLSDADGVHVGLEDAPVDEVKRRFSGIIIGASASTVDEAKEGEKAG
CCCEEEEECCCCEEEEECCCCEEECCCCCCHHHHHHHHCCEEEECCCCHHHHHHCCHHCC
ADYLGAGSIFPSPTKPDYRILGLEGLRRVVQSVSIPVYAIGGVTLESIPAIKATGAWGAA
CCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCEEEECCEEHHHCCCEEECCCHHHH
VISGILAAKDPLEMAKRFVKAWDEA
HHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLPKGIYGITDDSYNVKNHVDAAKVFLEGGVRIIQYRRKEGSIRQMLNEAKEIRKLCNQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEHHCCCHHHHHHHHHHHHHHHHHH
YGAVMIVDDRVDIAVLSDADGVHVGLEDAPVDEVKRRFSGIIIGASASTVDEAKEGEKAG
CCCEEEEECCCCEEEEECCCCEEECCCCCCHHHHHHHHCCEEEECCCCHHHHHHCCHHCC
ADYLGAGSIFPSPTKPDYRILGLEGLRRVVQSVSIPVYAIGGVTLESIPAIKATGAWGAA
CCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCEEEECCEEHHHCCCEEECCCHHHH
VISGILAAKDPLEMAKRFVKAWDEA
HHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA