Click here to switch to the map view.

The map label for this gene is thiM

Identifier: 159040654

GI number: 159040654

Start: 76058

End: 76858

Strand: Reverse

Name: thiM

Synonym: Cmaq_0062

Alternate gene names: 159040654

Gene position: 76858-76058 (Counterclockwise)

Preceding gene: 159040655

Following gene: 159040653

Centisome position: 3.7

GC content: 44.57

Gene sequence:

>801_bases
ATGTGGTTAAGCGACGAGTTTCCAAGGGACCTAAAAACCCTTAGGGATAGGAAACCACTGGTACATCATTTCATGAACTT
CGTTGTAATGAATGATGCAGCCAACGTCACCTTAGCCATAGGTGCATCACCAATAATGGCTCATGCAAAGGAGGAGGTTG
AGGAATTAGCCAGTAAGGCTAATGCACTATACATAAACATAGGCACACTAGATGAGTATTGGGTTGAATCAATGATAATT
GCAGGCAAGGCAGCTAGTAGAAATAATGTTCCAATACTCCTAGATCCAGTGGGCGCTGGGGCCACTAGGTATAGGACTGA
GGTTGTTAAACGCATATTAAGTGAAACTGAGGTATCAGTGGTTAAGGGTAATGGAGGTGAAATGCTAGCCCTAGCCGGTG
TAACAGGTGGCGTCAAGGGGGTTGATTCAATAGTCAACGCAACAATGGATACGGCGGTTAAGATTGCTGAGGAATACGGT
GTAACAGCAGTGATTACTGGTCCATGGGATTACGTATCAGATGGTGTTAGGAAGGTCATTGTTAAGAATGGTACACCACT
ACTCCAATACGTAACCGGCTCCGGCTGCATGGTTGGTTCAGTTATAGCAAGCTTCATGGCGATTAACAGGGATTTCGTTA
AGGCTTCAGTAGAGGGACTGGTTGCCTTTGAAATAGCTGCTGAGAAGGCTGAGGCTAAGAGTAAGGCACCTGGTACATTT
AAACAGTACCTAATTGATGAGTTATTTAACCTAACCGGTGACGATTACGTTAAAATGGCTAAGGTAGAGGTGGTTCAGTG
A

Upstream 100 bases:

>100_bases
GTTTACTCAAGCGAGTACTAGGGTGTGTTAACTATAATTAATTTATTGATTATTAATAACTTGCGAATGATTTAAAAAGA
GGACACTCAAGAATCCACCA

Downstream 100 bases:

>100_bases
GATTACCTAAGGGCATATACGGTATAACTGATGACTCATATAATGTTAAGAACCATGTGGATGCAGCCAAGGTGTTCCTA
GAGGGGGGTGTGAGGATAAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MWLSDEFPRDLKTLRDRKPLVHHFMNFVVMNDAANVTLAIGASPIMAHAKEEVEELASKANALYINIGTLDEYWVESMII
AGKAASRNNVPILLDPVGAGATRYRTEVVKRILSETEVSVVKGNGGEMLALAGVTGGVKGVDSIVNATMDTAVKIAEEYG
VTAVITGPWDYVSDGVRKVIVKNGTPLLQYVTGSGCMVGSVIASFMAINRDFVKASVEGLVAFEIAAEKAEAKSKAPGTF
KQYLIDELFNLTGDDYVKMAKVEVVQ

Sequences:

>Translated_266_residues
MWLSDEFPRDLKTLRDRKPLVHHFMNFVVMNDAANVTLAIGASPIMAHAKEEVEELASKANALYINIGTLDEYWVESMII
AGKAASRNNVPILLDPVGAGATRYRTEVVKRILSETEVSVVKGNGGEMLALAGVTGGVKGVDSIVNATMDTAVKIAEEYG
VTAVITGPWDYVSDGVRKVIVKNGTPLLQYVTGSGCMVGSVIASFMAINRDFVKASVEGLVAFEIAAEKAEAKSKAPGTF
KQYLIDELFNLTGDDYVKMAKVEVVQ
>Mature_266_residues
MWLSDEFPRDLKTLRDRKPLVHHFMNFVVMNDAANVTLAIGASPIMAHAKEEVEELASKANALYINIGTLDEYWVESMII
AGKAASRNNVPILLDPVGAGATRYRTEVVKRILSETEVSVVKGNGGEMLALAGVTGGVKGVDSIVNATMDTAVKIAEEYG
VTAVITGPWDYVSDGVRKVIVKNGTPLLQYVTGSGCMVGSVIASFMAINRDFVKASVEGLVAFEIAAEKAEAKSKAPGTF
KQYLIDELFNLTGDDYVKMAKVEVVQ

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=244, Percent_Identity=36.4754098360656, Blast_Score=155, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325042, Length=285, Percent_Identity=33.6842105263158, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CALMQ (A8M9N5)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001539906.1
- ProteinModelPortal:   A8M9N5
- SMR:   A8M9N5
- GeneID:   5710231
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0062
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- BioCyc:   CMAQ397948:CMAQ_0062-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28648; Mature: 28648

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: BINDING 46-46 BINDING 122-122 BINDING 166-166 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLSDEFPRDLKTLRDRKPLVHHFMNFVVMNDAANVTLAIGASPIMAHAKEEVEELASKA
CCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCC
NALYINIGTLDEYWVESMIIAGKAASRNNVPILLDPVGAGATRYRTEVVKRILSETEVSV
CEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VKGNGGEMLALAGVTGGVKGVDSIVNATMDTAVKIAEEYGVTAVITGPWDYVSDGVRKVI
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHEEH
VKNGTPLLQYVTGSGCMVGSVIASFMAINRDFVKASVEGLVAFEIAAEKAEAKSKAPGTF
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCCCCHH
KQYLIDELFNLTGDDYVKMAKVEVVQ
HHHHHHHHHCCCCCCCEEEHEEEECC
>Mature Secondary Structure
MWLSDEFPRDLKTLRDRKPLVHHFMNFVVMNDAANVTLAIGASPIMAHAKEEVEELASKA
CCCCCCCCHHHHHHHCCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCC
NALYINIGTLDEYWVESMIIAGKAASRNNVPILLDPVGAGATRYRTEVVKRILSETEVSV
CEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VKGNGGEMLALAGVTGGVKGVDSIVNATMDTAVKIAEEYGVTAVITGPWDYVSDGVRKVI
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHEEH
VKNGTPLLQYVTGSGCMVGSVIASFMAINRDFVKASVEGLVAFEIAAEKAEAKSKAPGTF
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCCCCHH
KQYLIDELFNLTGDDYVKMAKVEVVQ
HHHHHHHHHCCCCCCCEEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA