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The map label for this gene is lysE [H]

Identifier: 158425634

GI number: 158425634

Start: 4576036

End: 4576692

Strand: Reverse

Name: lysE [H]

Synonym: AZC_4010

Alternate gene names: 158425634

Gene position: 4576692-4576036 (Counterclockwise)

Preceding gene: 158425635

Following gene: 158425633

Centisome position: 85.23

GC content: 67.73

Gene sequence:

>657_bases
ATGAGCAGCCATCTCGCGGAATTCCTGACCATCATGGCAGTGTTTTCCCTGGCCGCCGTGACACCGGGACCGGACTTCGC
CATGGTGGTGCGCCAGAGCGTGGTGCGGGGCCGCCGCGCGGGCATCATCACCAGCTTCGGCATCGGCGCCGCCATCTTCT
TCCATGCGGCCTATACGGTGGCCGGGATCGGCCTGCTGGTCGCCCACTCGATCCTCGCCTTCAATGTGCTGAAATGGGCG
GGTGCCGCCTATCTCTTCTACGTGGGCGTGAAGACCTGGCGCGCGCCGCCACCCAGCTATCAGGTGGATCTTGCGGACGC
GCCCGCGCCGGCGGTTGCCCCCCGGCGCTACGGCGATGCGTCCGACTTCACGCTCGGGTTCCTCACCAATGCGCTGAACC
CGAAGGCGGTGCTGTTCTTCCTCTCCATCTTCACCACGGTGGTGAGCCCCACCACGCCGCAGAGCATGCAGATGGGCTAT
GCCGCCGGCATGGCCGGCCTGCTGGTCGTCTGGTTCACGCTGGTCTCGGTGTTCTTCACCGGGCGGGCGGTGCAGGCGCA
GCTCTCGCGCATCGGCCGCTGGTTCAATCGCATCACCGGCGCGGCCCTGGTCGGCCTCGGCATCCGCCTCGCCTTCCAGA
GGGCCTCCGCCCACTGA

Upstream 100 bases:

>100_bases
CTCTGTCGCCGCGCCGTTTCCCGACCGGGCGTCCGCCGCCCACTGACAGGCCGGTTGCGGCAGGGCCGCGCCCCACGGCC
GCTGACACGAGGACGGGCGA

Downstream 100 bases:

>100_bases
TCCCTTCCCGTTCGCTGCGGCCGGCTGGCCGCCCGCTTCCTTCAAGGACTGTATTCCATGGCTAATTACGACCTGATCGT
CATCGGCACCGGCCCCGGCG

Product: amino acid efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSSHLAEFLTIMAVFSLAAVTPGPDFAMVVRQSVVRGRRAGIITSFGIGAAIFFHAAYTVAGIGLLVAHSILAFNVLKWA
GAAYLFYVGVKTWRAPPPSYQVDLADAPAPAVAPRRYGDASDFTLGFLTNALNPKAVLFFLSIFTTVVSPTTPQSMQMGY
AAGMAGLLVVWFTLVSVFFTGRAVQAQLSRIGRWFNRITGAALVGLGIRLAFQRASAH

Sequences:

>Translated_218_residues
MSSHLAEFLTIMAVFSLAAVTPGPDFAMVVRQSVVRGRRAGIITSFGIGAAIFFHAAYTVAGIGLLVAHSILAFNVLKWA
GAAYLFYVGVKTWRAPPPSYQVDLADAPAPAVAPRRYGDASDFTLGFLTNALNPKAVLFFLSIFTTVVSPTTPQSMQMGY
AAGMAGLLVVWFTLVSVFFTGRAVQAQLSRIGRWFNRITGAALVGLGIRLAFQRASAH
>Mature_217_residues
SSHLAEFLTIMAVFSLAAVTPGPDFAMVVRQSVVRGRRAGIITSFGIGAAIFFHAAYTVAGIGLLVAHSILAFNVLKWAG
AAYLFYVGVKTWRAPPPSYQVDLADAPAPAVAPRRYGDASDFTLGFLTNALNPKAVLFFLSIFTTVVSPTTPQSMQMGYA
AGMAGLLVVWFTLVSVFFTGRAVQAQLSRIGRWFNRITGAALVGLGIRLAFQRASAH

Specific function: Conducts The Efflux Of Threonine. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=210, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786522, Length=208, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994979, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788099, Length=198, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23277; Mature: 23145

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHLAEFLTIMAVFSLAAVTPGPDFAMVVRQSVVRGRRAGIITSFGIGAAIFFHAAYTV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHH
AGIGLLVAHSILAFNVLKWAGAAYLFYVGVKTWRAPPPSYQVDLADAPAPAVAPRRYGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCC
SDFTLGFLTNALNPKAVLFFLSIFTTVVSPTTPQSMQMGYAAGMAGLLVVWFTLVSVFFT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRAVQAQLSRIGRWFNRITGAALVGLGIRLAFQRASAH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSHLAEFLTIMAVFSLAAVTPGPDFAMVVRQSVVRGRRAGIITSFGIGAAIFFHAAYTV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHH
AGIGLLVAHSILAFNVLKWAGAAYLFYVGVKTWRAPPPSYQVDLADAPAPAVAPRRYGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCC
SDFTLGFLTNALNPKAVLFFLSIFTTVVSPTTPQSMQMGYAAGMAGLLVVWFTLVSVFFT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRAVQAQLSRIGRWFNRITGAALVGLGIRLAFQRASAH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9308178 [H]