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The map label for this gene is sucB [H]

Identifier: 158425635

GI number: 158425635

Start: 4576821

End: 4578059

Strand: Reverse

Name: sucB [H]

Synonym: AZC_4011

Alternate gene names: 158425635

Gene position: 4578059-4576821 (Counterclockwise)

Preceding gene: 158425636

Following gene: 158425634

Centisome position: 85.26

GC content: 69.9

Gene sequence:

>1239_bases
ATGGCGACCGAAATCCGCGTGCCCACCCTGGGCGAGTCCGTTACCGAGGCCACCATCGGCAAGTGGTTCAAGAAGCCTGG
CGATGCGGTGAAGGCCGACGAGCCGCTGGTGGAACTGGAGACCGACAAGGTCACCGTCGAGGTTCCCGCCCCGGCCGCTG
GCGTCCTCTCCGAAATCATCGCCAAGGACGGTGACACCGTCGGCGTCGGCGCCCTCCTCGGCGCGATCGCCGAAGGCGCT
GCCGGCGCTGCTGCCGCCGCTCCGAAGGCCGAGGCCCCCAAGGCGGCTCCGGCTCCGGCCGCTGCGCCCGCTCCGGCGCC
TGCCGCCGCCCCGGCCAAGGCCGCTTCCGGCGCCAATGGCCCGGCGGTCGAGCGTCTCGCCGCCGAGACCGGCGTGTCCG
CCGCCAACGTCGCCGGCTCCGGCAAGGATGGCCGCGTGACCAAGGGCGACATGCTGGCCGCCATCGCCTCCGGCGCTGCC
GCTCCGGCCGCTGCCCCGGCGCCGGTCGCCGTCCGCGCCCCCTCCGCGCCGGTGGATGCCGCGCGCGAAGAGCGCGTGAA
GATGACCAAGCTGCGGCAGACCATCGCCCGCCGCCTGAAGGACGCCCAGAACACCGCCGCCATGCTCACCACGTTCAACG
ACGTGGACATGAGCGCGGTGATGGGCCTGCGCGCCCAGTTCAAGGACTCCTTCGAGAAGAAGCACGGCACCAAGCTCGGC
TTCATGGGCTTCTTCACCAAGGCCGTCATCGCGGCCCTGAAGGACCTGCCGGCGGTGAACGCCGAGATCGACGGTCAGGA
CATCGTCTACAAGAACTACTACAACATCGGTATCGCGGTGGGCACCGAGAAGGGCCTCGTGGTCCCGGTGGTGCGTGACG
CCGACGAGCTGTCGGTGGCCGGCATCGAGAAGGCCATCGCCGGCTTCGGCCGCAAGGCCCGTGACGGCAAGCTCGGCATC
GAGGACATGCAGGGCGGCACCTTCACGATCACCAACGGCGGCATCTACGGCTCGCTGATGTCCACCCCCATCCTCAACGC
GCCGCAGTCCGGCATCCTCGGCATGCACCGCATCGAGGAGCGTCCGGTGGTGGTCAAGGGCCAGATCGTCGTGCGCCCGA
TGATGTATCTCGCGCTCTCCTACGACCACCGCATCGTCGACGGCCGCGAGGCCGTGACCTTCCTGGTGCGCGTGAAGGAG
ACGCTGGAGGATCCCGCGCGTCTCGTGCTCGACCTCTGA

Upstream 100 bases:

>100_bases
TCGGCTTCTCCTATGAGGTGCTGCCGGTTCCATCCCTTATTTTGCGCCGGGGTTGACCCGGCATCGAGCGTTTGAGGGAG
GCCCCCCGCGGGCGAATGTG

Downstream 100 bases:

>100_bases
TCGTCCAAGCCTGACACCGGCGCGGCGGCTCTGTCGCCGCGCCGTTTCCCGACCGGGCGTCCGCCGCCCACTGACAGGCC
GGTTGCGGCAGGGCCGCGCC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MATEIRVPTLGESVTEATIGKWFKKPGDAVKADEPLVELETDKVTVEVPAPAAGVLSEIIAKDGDTVGVGALLGAIAEGA
AGAAAAAPKAEAPKAAPAPAAAPAPAPAAAPAKAASGANGPAVERLAAETGVSAANVAGSGKDGRVTKGDMLAAIASGAA
APAAAPAPVAVRAPSAPVDAAREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMGLRAQFKDSFEKKHGTKLG
FMGFFTKAVIAALKDLPAVNAEIDGQDIVYKNYYNIGIAVGTEKGLVVPVVRDADELSVAGIEKAIAGFGRKARDGKLGI
EDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVVVKGQIVVRPMMYLALSYDHRIVDGREAVTFLVRVKE
TLEDPARLVLDL

Sequences:

>Translated_412_residues
MATEIRVPTLGESVTEATIGKWFKKPGDAVKADEPLVELETDKVTVEVPAPAAGVLSEIIAKDGDTVGVGALLGAIAEGA
AGAAAAAPKAEAPKAAPAPAAAPAPAPAAAPAKAASGANGPAVERLAAETGVSAANVAGSGKDGRVTKGDMLAAIASGAA
APAAAPAPVAVRAPSAPVDAAREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMGLRAQFKDSFEKKHGTKLG
FMGFFTKAVIAALKDLPAVNAEIDGQDIVYKNYYNIGIAVGTEKGLVVPVVRDADELSVAGIEKAIAGFGRKARDGKLGI
EDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVVVKGQIVVRPMMYLALSYDHRIVDGREAVTFLVRVKE
TLEDPARLVLDL
>Mature_411_residues
ATEIRVPTLGESVTEATIGKWFKKPGDAVKADEPLVELETDKVTVEVPAPAAGVLSEIIAKDGDTVGVGALLGAIAEGAA
GAAAAAPKAEAPKAAPAPAAAPAPAPAAAPAKAASGANGPAVERLAAETGVSAANVAGSGKDGRVTKGDMLAAIASGAAA
PAAAPAPVAVRAPSAPVDAAREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMGLRAQFKDSFEKKHGTKLGF
MGFFTKAVIAALKDLPAVNAEIDGQDIVYKNYYNIGIAVGTEKGLVVPVVRDADELSVAGIEKAIAGFGRKARDGKLGIE
DMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVVVKGQIVVRPMMYLALSYDHRIVDGREAVTFLVRVKET
LEDPARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=256, Percent_Identity=55.078125, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=28.6713286713287, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=28.476821192053, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.6975717439294, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=28.8288288288288, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=34.7305389221557, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=50.1216545012166, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=36.2352941176471, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=45.320197044335, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.2517006802721, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=27.9342723004695, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=29.4498381877023, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=46.4547677261614, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=25.2771618625277, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24645909, Length=240, Percent_Identity=58.3333333333333, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=28.6384976525822, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129315, Length=234, Percent_Identity=32.9059829059829, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24582497, Length=234, Percent_Identity=32.9059829059829, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42373; Mature: 42242

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVTEATIGKWFKKPGDAVKADEPLVELETDKVTVEVPAPAAGVLSEII
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHH
AKDGDTVGVGALLGAIAEGAAGAAAAAPKAEAPKAAPAPAAAPAPAPAAAPAKAASGANG
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
PAVERLAAETGVSAANVAGSGKDGRVTKGDMLAAIASGAAAPAAAPAPVAVRAPSAPVDA
HHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCH
AREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMGLRAQFKDSFEKKHGTKLG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEH
FMGFFTKAVIAALKDLPAVNAEIDGQDIVYKNYYNIGIAVGTEKGLVVPVVRDADELSVA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHH
GIEKAIAGFGRKARDGKLGIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEE
HHHHHHHHCCCCCCCCCCCCEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHCC
RPVVVKGQIVVRPMMYLALSYDHRIVDGREAVTFLVRVKETLEDPARLVLDL
CCEEEECHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHEECC
>Mature Secondary Structure 
ATEIRVPTLGESVTEATIGKWFKKPGDAVKADEPLVELETDKVTVEVPAPAAGVLSEII
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHH
AKDGDTVGVGALLGAIAEGAAGAAAAAPKAEAPKAAPAPAAAPAPAPAAAPAKAASGANG
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
PAVERLAAETGVSAANVAGSGKDGRVTKGDMLAAIASGAAAPAAAPAPVAVRAPSAPVDA
HHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCH
AREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMGLRAQFKDSFEKKHGTKLG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEH
FMGFFTKAVIAALKDLPAVNAEIDGQDIVYKNYYNIGIAVGTEKGLVVPVVRDADELSVA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHH
GIEKAIAGFGRKARDGKLGIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEE
HHHHHHHHCCCCCCCCCCCCEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHCC
RPVVVKGQIVVRPMMYLALSYDHRIVDGREAVTFLVRVKETLEDPARLVLDL
CCEEEECHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]